In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces
Cystic echinococcosis (CE) remains an important challenge both in humans and animals. There is no safe and suitable remedy for CE, so the discovery of new compounds with promising scolicidal effects, particularly from herbal sources, is of great importance for therapeutic uses in the treatment and p...
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/16/3593 |
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| author | Mohammad Reza Youssefi Ali Nikpay Niloufar Hassanpour Aida Mirzapour Parisa Saleh Tabari Roman Pavela Filippo Maggi Riccardo Petrelli |
| author_facet | Mohammad Reza Youssefi Ali Nikpay Niloufar Hassanpour Aida Mirzapour Parisa Saleh Tabari Roman Pavela Filippo Maggi Riccardo Petrelli |
| author_sort | Mohammad Reza Youssefi |
| collection | DOAJ |
| description | Cystic echinococcosis (CE) remains an important challenge both in humans and animals. There is no safe and suitable remedy for CE, so the discovery of new compounds with promising scolicidal effects, particularly from herbal sources, is of great importance for therapeutic uses in the treatment and prevention of CE reappearance. Sesquiterpenes are C15 organic compounds made up of three isoprene units and mostly occurring as fragrant components of essential oils. They are of economic importance for the cosmetic and pharmaceutical industry, and recently attracted the attention of the scientific community for their remarkable parasiticidal properties. In the present study, we have focused on three known sesquiterpenes, isofuranodiene (IFD), α-bisabolol (BSB), and farnesol (FOH), as important phytoconstituents of the essential oils of wild celery (<i>Smyrnium olusatrum</i>), chamomile (<i>Matricaria chamomilla</i>), and acacia farnese (<i>Vachellia farnesiana</i>), respectively. Protoscoleces were recovered from fertile hydatid cysts and were exposed to different concentrations of the three tested compounds for different exposure times. The viability of protoscoleces was confirmed by 0.1% eosin staining. Results of scolicidal activity evaluations showed that IFD possessed the best effect against <i>Echinococcus granulosus</i> protoscoleces (LC<sub>50</sub> and LC<sub>90</sub> values of 8.87 and 25.48 µg/mL, respectively), followed by BSB (LC<sub>50</sub> of 103.2 µg/mL) and FOH (LC<sub>50</sub> of 113.68 µg/mL). The overall toxicity of IFD differed significantly from those of FOH and BSB, while there was no significant difference in toxicity between the latter compounds (<i>p</i> > 0.05). The present study showed that IFD seems to be a promising scolicidal agent and can be further tested to become a candidate for CE treatment. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T17:49:20Z |
| publishDate | 2020-08-01 |
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| spelling | doaj.art-e3135f6b2ca24352800a5456ad54c2822023-11-20T09:26:35ZengMDPI AGMolecules1420-30492020-08-012516359310.3390/molecules25163593In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> ProtoscolecesMohammad Reza Youssefi0Ali Nikpay1Niloufar Hassanpour2Aida Mirzapour3Parisa Saleh Tabari4Roman Pavela5Filippo Maggi6Riccardo Petrelli7Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol 19585/466, IranFaculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol 4618649767, IranFaculty of Veterinary Medicine, Babol-Branch, Islamic Azad University, Babol 19585/466, IranFaculty of Veterinary Medicine, Babol-Branch, Islamic Azad University, Babol 19585/466, IranFaculty of Veterinary Medicine, Babol-Branch, Islamic Azad University, Babol 19585/466, IranCrop Research Institute, Drnovska 507, Ruzyne, 161 06 Prague 6, Czech RepublicSchool of Pharmacy, University of Camerino, via Sant’Agostino 1, 62032 Camerino, ItalySchool of Pharmacy, University of Camerino, via Sant’Agostino 1, 62032 Camerino, ItalyCystic echinococcosis (CE) remains an important challenge both in humans and animals. There is no safe and suitable remedy for CE, so the discovery of new compounds with promising scolicidal effects, particularly from herbal sources, is of great importance for therapeutic uses in the treatment and prevention of CE reappearance. Sesquiterpenes are C15 organic compounds made up of three isoprene units and mostly occurring as fragrant components of essential oils. They are of economic importance for the cosmetic and pharmaceutical industry, and recently attracted the attention of the scientific community for their remarkable parasiticidal properties. In the present study, we have focused on three known sesquiterpenes, isofuranodiene (IFD), α-bisabolol (BSB), and farnesol (FOH), as important phytoconstituents of the essential oils of wild celery (<i>Smyrnium olusatrum</i>), chamomile (<i>Matricaria chamomilla</i>), and acacia farnese (<i>Vachellia farnesiana</i>), respectively. Protoscoleces were recovered from fertile hydatid cysts and were exposed to different concentrations of the three tested compounds for different exposure times. The viability of protoscoleces was confirmed by 0.1% eosin staining. Results of scolicidal activity evaluations showed that IFD possessed the best effect against <i>Echinococcus granulosus</i> protoscoleces (LC<sub>50</sub> and LC<sub>90</sub> values of 8.87 and 25.48 µg/mL, respectively), followed by BSB (LC<sub>50</sub> of 103.2 µg/mL) and FOH (LC<sub>50</sub> of 113.68 µg/mL). The overall toxicity of IFD differed significantly from those of FOH and BSB, while there was no significant difference in toxicity between the latter compounds (<i>p</i> > 0.05). The present study showed that IFD seems to be a promising scolicidal agent and can be further tested to become a candidate for CE treatment.https://www.mdpi.com/1420-3049/25/16/3593isofuranodieneα-<i>b</i>isabololfarnesolcystic echinococcosisprotoscolex |
| spellingShingle | Mohammad Reza Youssefi Ali Nikpay Niloufar Hassanpour Aida Mirzapour Parisa Saleh Tabari Roman Pavela Filippo Maggi Riccardo Petrelli In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces Molecules isofuranodiene α-<i>b</i>isabolol farnesol cystic echinococcosis protoscolex |
| title | In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces |
| title_full | In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces |
| title_fullStr | In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces |
| title_full_unstemmed | In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces |
| title_short | In Vitro Scolicidal Activity of the Sesquiterpenes Isofuranodiene, α-Bisabolol and Farnesol on <i>Echinococcus granulosus</i> Protoscoleces |
| title_sort | in vitro scolicidal activity of the sesquiterpenes isofuranodiene α bisabolol and farnesol on i echinococcus granulosus i protoscoleces |
| topic | isofuranodiene α-<i>b</i>isabolol farnesol cystic echinococcosis protoscolex |
| url | https://www.mdpi.com/1420-3049/25/16/3593 |
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