Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi

Entomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review th...

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Main Authors: Lav Sharma, Nitin Bohra, Vishnu D. Rajput, Francisco Roberto Quiroz-Figueroa, Rupesh Kumar Singh, Guilhermina Marques
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/9/1/16
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author Lav Sharma
Nitin Bohra
Vishnu D. Rajput
Francisco Roberto Quiroz-Figueroa
Rupesh Kumar Singh
Guilhermina Marques
author_facet Lav Sharma
Nitin Bohra
Vishnu D. Rajput
Francisco Roberto Quiroz-Figueroa
Rupesh Kumar Singh
Guilhermina Marques
author_sort Lav Sharma
collection DOAJ
description Entomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review the current techniques that have been developed so far, in the last five decades, and display brief protocols which can be adopted for the isolations of these entomopathogens. Among bacteria, this review focuses on <i>Serratia</i> spp. and bacteria from the class Bacilli. Among fungi, the review focuses those from the order Hypocreales, for example, genera <i>Beauveria</i>, <i>Clonostachys</i>, <i>Lecanicillium</i>, <i>Metarhizium</i>, and <i>Purpureocillium</i>. The authors chose these groups of entomopathogenic bacteria and fungi based on their importance in the microbial biopesticide market.
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spelling doaj.art-e31466aadd954683829be6b063a890822023-11-21T02:09:37ZengMDPI AGMicroorganisms2076-26072020-12-01911610.3390/microorganisms9010016Advances in Entomopathogen Isolation: A Case of Bacteria and FungiLav Sharma0Nitin Bohra1Vishnu D. Rajput2Francisco Roberto Quiroz-Figueroa3Rupesh Kumar Singh4Guilhermina Marques5Centre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalMax Planck School Matter to Life, Max Planck Institute for Medical Research, Jahnstraße 29, 69120 Heidelberg, GermanySoil Science and Land Evaluation Department, Academy of Biology and Biotechnology, Southern Federal University, 344090 Rostov-on-Don, RussiaInstituto Politécnico Nacional, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-IPN Unidad Sinaloa), Laboratorio de Fitomejoramiento Molecular, Blvd. Juan de Dios Bátiz Paredes no. 250, Col. San Joachín, C.P., Guasave 81101, MexicoCentro de Química de Vila Real, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalCentre for the Research and Technology of Agro-Environment and Biological Sciences, Universidade de Trás-os-Montes e Alto Douro, Quinta de Prados, 5000-801 Vila Real, PortugalEntomopathogenic bacteria and fungi are quite frequently found in soils and insect cadavers. The first step in utilizing these microbes as biopesticides is to isolate them, and several culture media and insect baiting procedures have been tested in this direction. In this work, the authors review the current techniques that have been developed so far, in the last five decades, and display brief protocols which can be adopted for the isolations of these entomopathogens. Among bacteria, this review focuses on <i>Serratia</i> spp. and bacteria from the class Bacilli. Among fungi, the review focuses those from the order Hypocreales, for example, genera <i>Beauveria</i>, <i>Clonostachys</i>, <i>Lecanicillium</i>, <i>Metarhizium</i>, and <i>Purpureocillium</i>. The authors chose these groups of entomopathogenic bacteria and fungi based on their importance in the microbial biopesticide market.https://www.mdpi.com/2076-2607/9/1/16<i>Beauveria</i><i>Metarhizium</i>Hypocreales<i>Bacillus thuringiensis</i><i>Serratia</i>
spellingShingle Lav Sharma
Nitin Bohra
Vishnu D. Rajput
Francisco Roberto Quiroz-Figueroa
Rupesh Kumar Singh
Guilhermina Marques
Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
Microorganisms
<i>Beauveria</i>
<i>Metarhizium</i>
Hypocreales
<i>Bacillus thuringiensis</i>
<i>Serratia</i>
title Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
title_full Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
title_fullStr Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
title_full_unstemmed Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
title_short Advances in Entomopathogen Isolation: A Case of Bacteria and Fungi
title_sort advances in entomopathogen isolation a case of bacteria and fungi
topic <i>Beauveria</i>
<i>Metarhizium</i>
Hypocreales
<i>Bacillus thuringiensis</i>
<i>Serratia</i>
url https://www.mdpi.com/2076-2607/9/1/16
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AT vishnudrajput advancesinentomopathogenisolationacaseofbacteriaandfungi
AT franciscorobertoquirozfigueroa advancesinentomopathogenisolationacaseofbacteriaandfungi
AT rupeshkumarsingh advancesinentomopathogenisolationacaseofbacteriaandfungi
AT guilherminamarques advancesinentomopathogenisolationacaseofbacteriaandfungi