Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts
In this study, inhibition ability of Veronica officinalis extracts against Alzheimer’s disease-related enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α- amylase and antioxidant of Veronica officinalis were investigated. To...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siirt University
2018-10-01
|
| Series: | Türkiye Tarımsal Araştırmalar Dergisi |
| Subjects: | |
| Online Access: | http://dergipark.gov.tr/tutad/issue/38790/425732 |
| _version_ | 1797914984643035136 |
|---|---|
| author | Nuraniye ERUYGUR Esra UÇAR |
| author_facet | Nuraniye ERUYGUR Esra UÇAR |
| author_sort | Nuraniye ERUYGUR |
| collection | DOAJ |
| description | In this study, inhibition ability of Veronica officinalis extracts against Alzheimer’s disease-related enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α-
amylase and antioxidant of Veronica officinalis were investigated. To the best of our knowledge, there are no previous studies
on the enzyme inhibition activities of the V. officinalis extracts. For this aim, V. officinalis extracted with methanol and water
by maceration method and their antioxidant activities were evaluated by DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS [2,2′-
Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging assays, total phenol, total flavonoid content, iron
chelating and ferric reducing power assays. The enzyme inhibition activity was performed with 96-well plate method.
According to the obtained results; the plant extracts have good antioxidant effects. In the same time, the water extract have
stronger inhibition activity against AChE, BuChE and α-glucosidase, α-amylase, and tyrosinase than methanol extract. The
experimental findings indicated that the water extract exerted the higher α-glucosidase, α-amylase and tyrosinase (82.07 ±
2.91, 63.61 ± 3.93 and 47.47 ± 0.53 at 2 mg mL-1, respectively) inhibition than reference drugs. The obtained results
demonstrate that this plant has a significant potential for improving pharmaceutical formulations. |
| first_indexed | 2024-04-10T12:34:38Z |
| format | Article |
| id | doaj.art-e317ad4f19fa4f67b59fb184cf5d22c2 |
| institution | Directory Open Access Journal |
| issn | 2148-2306 2528-858X |
| language | English |
| last_indexed | 2024-04-10T12:34:38Z |
| publishDate | 2018-10-01 |
| publisher | Siirt University |
| record_format | Article |
| series | Türkiye Tarımsal Araştırmalar Dergisi |
| spelling | doaj.art-e317ad4f19fa4f67b59fb184cf5d22c22023-02-15T16:14:42ZengSiirt UniversityTürkiye Tarımsal Araştırmalar Dergisi2148-23062528-858X2018-10-015325325910.19159/tutad.425732Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis ExtractsNuraniye ERUYGUR0Esra UÇAR1Selçuk University Faculty of Pharmacy, Department of Pharmacognosy, Konya, TURKEYCumhuriyet University, Sivas Vocational School, Department of Crop and Animal Production, Sivas, TURKEYIn this study, inhibition ability of Veronica officinalis extracts against Alzheimer’s disease-related enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α- amylase and antioxidant of Veronica officinalis were investigated. To the best of our knowledge, there are no previous studies on the enzyme inhibition activities of the V. officinalis extracts. For this aim, V. officinalis extracted with methanol and water by maceration method and their antioxidant activities were evaluated by DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS [2,2′- Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging assays, total phenol, total flavonoid content, iron chelating and ferric reducing power assays. The enzyme inhibition activity was performed with 96-well plate method. According to the obtained results; the plant extracts have good antioxidant effects. In the same time, the water extract have stronger inhibition activity against AChE, BuChE and α-glucosidase, α-amylase, and tyrosinase than methanol extract. The experimental findings indicated that the water extract exerted the higher α-glucosidase, α-amylase and tyrosinase (82.07 ± 2.91, 63.61 ± 3.93 and 47.47 ± 0.53 at 2 mg mL-1, respectively) inhibition than reference drugs. The obtained results demonstrate that this plant has a significant potential for improving pharmaceutical formulations.http://dergipark.gov.tr/tutad/issue/38790/425732Antioxidantalzheimer’sdiabetestyrosinaseVeronica officinalis |
| spellingShingle | Nuraniye ERUYGUR Esra UÇAR Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts Türkiye Tarımsal Araştırmalar Dergisi Antioxidant alzheimer’s diabetes tyrosinase Veronica officinalis |
| title | Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts |
| title_full | Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts |
| title_fullStr | Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts |
| title_full_unstemmed | Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts |
| title_short | Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts |
| title_sort | cholinesterase α glucosidase α amylase and tyrosinase inhibitory effects and antioxidant activity of veronica officinalis extracts |
| topic | Antioxidant alzheimer’s diabetes tyrosinase Veronica officinalis |
| url | http://dergipark.gov.tr/tutad/issue/38790/425732 |
| work_keys_str_mv | AT nuraniyeeruygur cholinesteraseaglucosidaseaamylaseandtyrosinaseinhibitoryeffectsandantioxidantactivityofveronicaofficinalisextracts AT esraucar cholinesteraseaglucosidaseaamylaseandtyrosinaseinhibitoryeffectsandantioxidantactivityofveronicaofficinalisextracts |