MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities
Mastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. In this study, mastoparan V1 (MP-V1) from Vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties a...
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| Format: | Article |
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MDPI AG
2016-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/4/512 |
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| author | Yangseon Kim Minky Son Eun-Young Noh Soonok Kim Changmu Kim Joo-Hong Yeo Chanin Park Keun Woo Lee Woo Young Bang |
| author_facet | Yangseon Kim Minky Son Eun-Young Noh Soonok Kim Changmu Kim Joo-Hong Yeo Chanin Park Keun Woo Lee Woo Young Bang |
| author_sort | Yangseon Kim |
| collection | DOAJ |
| description | Mastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. In this study, mastoparan V1 (MP-V1) from Vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties and antimicrobial effects were compared with those of typical mastoparans MP-L, -X(V) and -B. Circular dichroism (CD) spectroscopy revealed that MP-V1 and -X(V) form more stable α-helical conformations in lipid membrane-like environments than MP-L and -B. In parallel, these two also showed more effective antimicrobial activities against the pathogens than did MP-L and -B. Although MP-V1 had a less stable α-helical conformation than MP-X(V), it showed stronger antimicrobial effects against Streptococcus mutans and Salmonella enterica than MP-X(V). In the meantime, analysis of hemolytic activity revealed a range of doses (~50 μM) that exhibited little potent cytotoxicity on human erythrocytes. Finally, the atypical MP-V1 peptide amino acid sequence provided important clues to understanding its antimicrobial mechanism from a structural perspective. Therefore, it has been concluded that MP-V1 is a de novo type of mastoparan with superior antimicrobial activities against both pathogenic bacteria and fungi, which may be useful in developing multipurpose antimicrobial drugs against infectious diseases. |
| first_indexed | 2024-12-22T19:36:42Z |
| format | Article |
| id | doaj.art-e3190639328045d9939c515eaecf1fe8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T19:36:42Z |
| publishDate | 2016-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e3190639328045d9939c515eaecf1fe82022-12-21T18:14:57ZengMDPI AGMolecules1420-30492016-04-0121451210.3390/molecules21040512molecules21040512MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial ActivitiesYangseon Kim0Minky Son1Eun-Young Noh2Soonok Kim3Changmu Kim4Joo-Hong Yeo5Chanin Park6Keun Woo Lee7Woo Young Bang8National Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaDivision of Applied Life Science (BK21 Plus) & Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, KoreaNational Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaNational Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaNational Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaNational Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaDivision of Applied Life Science (BK21 Plus) & Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, KoreaDivision of Applied Life Science (BK21 Plus) & Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University (GNU), 501 Jinju-daero, Jinju 660-701, KoreaNational Institute of Biological Resources (NIBR), Environmental Research Complex, Incheon 404-708, KoreaMastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. In this study, mastoparan V1 (MP-V1) from Vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties and antimicrobial effects were compared with those of typical mastoparans MP-L, -X(V) and -B. Circular dichroism (CD) spectroscopy revealed that MP-V1 and -X(V) form more stable α-helical conformations in lipid membrane-like environments than MP-L and -B. In parallel, these two also showed more effective antimicrobial activities against the pathogens than did MP-L and -B. Although MP-V1 had a less stable α-helical conformation than MP-X(V), it showed stronger antimicrobial effects against Streptococcus mutans and Salmonella enterica than MP-X(V). In the meantime, analysis of hemolytic activity revealed a range of doses (~50 μM) that exhibited little potent cytotoxicity on human erythrocytes. Finally, the atypical MP-V1 peptide amino acid sequence provided important clues to understanding its antimicrobial mechanism from a structural perspective. Therefore, it has been concluded that MP-V1 is a de novo type of mastoparan with superior antimicrobial activities against both pathogenic bacteria and fungi, which may be useful in developing multipurpose antimicrobial drugs against infectious diseases.http://www.mdpi.com/1420-3049/21/4/512antimicrobial activitymastoparanMP-V1peptidevenomwasp |
| spellingShingle | Yangseon Kim Minky Son Eun-Young Noh Soonok Kim Changmu Kim Joo-Hong Yeo Chanin Park Keun Woo Lee Woo Young Bang MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities Molecules antimicrobial activity mastoparan MP-V1 peptide venom wasp |
| title | MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities |
| title_full | MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities |
| title_fullStr | MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities |
| title_full_unstemmed | MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities |
| title_short | MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities |
| title_sort | mp v1 from the venom of social wasp vespula vulgaris is a de novo type of mastoparan that displays superior antimicrobial activities |
| topic | antimicrobial activity mastoparan MP-V1 peptide venom wasp |
| url | http://www.mdpi.com/1420-3049/21/4/512 |
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