Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity
Enterobacter cloacae B13 strain is a rod-shaped gram-negative bacterium that belongs to the Enterobacteriaceae family. It can cause respiratory and urinary tract infections, and is responsible for several outbreaks in hospitals. E. cloacae has become an important pathogen and an emerging global thre...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2022-08-01
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| Series: | Bioinformatics and Biology Insights |
| Online Access: | https://doi.org/10.1177/11779322221115535 |
| _version_ | 1811343964688613376 |
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| author | Supantha Dey Sazzad Shahrear Maliha Afroj Zinnia Ahnaf Tajwar Abul Bashar Mir Md. Khademul Islam |
| author_facet | Supantha Dey Sazzad Shahrear Maliha Afroj Zinnia Ahnaf Tajwar Abul Bashar Mir Md. Khademul Islam |
| author_sort | Supantha Dey |
| collection | DOAJ |
| description | Enterobacter cloacae B13 strain is a rod-shaped gram-negative bacterium that belongs to the Enterobacteriaceae family. It can cause respiratory and urinary tract infections, and is responsible for several outbreaks in hospitals. E. cloacae has become an important pathogen and an emerging global threat because of its opportunistic and multidrug resistant ability. However, little knowledge is present about a large portion of its proteins and functions. Therefore, functional annotation of the hypothetical proteins (HPs) can provide an improved understanding of this organism and its virulence activity. The workflow in the study included several bioinformatic tools which were utilized to characterize functions, family and domains, subcellular localization, physiochemical properties, and protein-protein interactions. The E. cloacae B13 strain has overall 604 HPs, among which 78 were functionally annotated with high confidence. Several proteins were identified as enzymes, regulatory, binding, and transmembrane proteins with essential functions. Furthermore, 23 HPs were predicted to be virulent factors. These virulent proteins are linked to pathogenesis with their contribution to biofilm formation, quorum sensing, 2-component signal transduction or secretion. Better knowledge about the HPs’ characteristics and functions will provide a greater overview of the proteome. Moreover, it will help against E. cloacae in neonatal intensive care unit (NICU) outbreaks and nosocomial infections. |
| first_indexed | 2024-04-13T19:39:21Z |
| format | Article |
| id | doaj.art-e31a0596bd544204af5dd39f1e6774da |
| institution | Directory Open Access Journal |
| issn | 1177-9322 |
| language | English |
| last_indexed | 2024-04-13T19:39:21Z |
| publishDate | 2022-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Bioinformatics and Biology Insights |
| spelling | doaj.art-e31a0596bd544204af5dd39f1e6774da2022-12-22T02:32:57ZengSAGE PublishingBioinformatics and Biology Insights1177-93222022-08-011610.1177/11779322221115535Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With PathogenicitySupantha Dey0Sazzad Shahrear1Maliha Afroj Zinnia2Ahnaf Tajwar3Abul Bashar Mir Md. Khademul Islam4Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, BangladeshDepartment of Pharmacy, East West University, Dhaka, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka, BangladeshEnterobacter cloacae B13 strain is a rod-shaped gram-negative bacterium that belongs to the Enterobacteriaceae family. It can cause respiratory and urinary tract infections, and is responsible for several outbreaks in hospitals. E. cloacae has become an important pathogen and an emerging global threat because of its opportunistic and multidrug resistant ability. However, little knowledge is present about a large portion of its proteins and functions. Therefore, functional annotation of the hypothetical proteins (HPs) can provide an improved understanding of this organism and its virulence activity. The workflow in the study included several bioinformatic tools which were utilized to characterize functions, family and domains, subcellular localization, physiochemical properties, and protein-protein interactions. The E. cloacae B13 strain has overall 604 HPs, among which 78 were functionally annotated with high confidence. Several proteins were identified as enzymes, regulatory, binding, and transmembrane proteins with essential functions. Furthermore, 23 HPs were predicted to be virulent factors. These virulent proteins are linked to pathogenesis with their contribution to biofilm formation, quorum sensing, 2-component signal transduction or secretion. Better knowledge about the HPs’ characteristics and functions will provide a greater overview of the proteome. Moreover, it will help against E. cloacae in neonatal intensive care unit (NICU) outbreaks and nosocomial infections.https://doi.org/10.1177/11779322221115535 |
| spellingShingle | Supantha Dey Sazzad Shahrear Maliha Afroj Zinnia Ahnaf Tajwar Abul Bashar Mir Md. Khademul Islam Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity Bioinformatics and Biology Insights |
| title | Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity |
| title_full | Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity |
| title_fullStr | Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity |
| title_full_unstemmed | Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity |
| title_short | Functional Annotation of Hypothetical Proteins From the B13 Strain and Its Association With Pathogenicity |
| title_sort | functional annotation of hypothetical proteins from the b13 strain and its association with pathogenicity |
| url | https://doi.org/10.1177/11779322221115535 |
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