Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives

The article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were de...

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Main Authors: Raeva-Bogoslovskaya Ekaterina N., Molkanova Olga I., Kryuchkova Viktoriya A.
Format: Article
Language:English
Published: EDP Sciences 2021-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/30/e3sconf_farba2021_04005.pdf
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author Raeva-Bogoslovskaya Ekaterina N.
Molkanova Olga I.
Kryuchkova Viktoriya A.
author_facet Raeva-Bogoslovskaya Ekaterina N.
Molkanova Olga I.
Kryuchkova Viktoriya A.
author_sort Raeva-Bogoslovskaya Ekaterina N.
collection DOAJ
description The article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were determined. The optimal auxin source for rhizogenesis of various Amelanchier alnifolia varieties has been established. Conditions for explants' preservation in aseptic conditions for 12 months have been revealed. Different effects of cytokinin type substances on shadberry regeneration potential under aseptic conditions were noted. Analysis of regenerants' morphometric indicators showed the effectiveness of using 6-benzylaminopurin (BAP) in the concentration of 1.0 mg/L for shadberry varieties' clonal micropropagation and indolebutyric acid (IBA) at 1.0 mg/L concentration for microshoots' rooting.
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spelling doaj.art-e31ba6c54a8c47e09bb38a5569d6c99f2022-12-21T22:31:42ZengEDP SciencesE3S Web of Conferences2267-12422021-01-012540400510.1051/e3sconf/202125404005e3sconf_farba2021_04005Some aspects of clonal micropropagation of Amelanchier Medik. genus representativesRaeva-Bogoslovskaya Ekaterina N.0Molkanova Olga I.1Kryuchkova Viktoriya A.2FSBIS Main Botanical Garden named after N.V. Tsitsin, RAS, Laboratory of Plant BiotechnologyFSBIS Main Botanical Garden named after N.V. Tsitsin, RAS, Laboratory of Plant BiotechnologyFSBIS Main Botanical Garden named after N.V. Tsitsin, RAS, Laboratory of Cultured PlantsThe article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were determined. The optimal auxin source for rhizogenesis of various Amelanchier alnifolia varieties has been established. Conditions for explants' preservation in aseptic conditions for 12 months have been revealed. Different effects of cytokinin type substances on shadberry regeneration potential under aseptic conditions were noted. Analysis of regenerants' morphometric indicators showed the effectiveness of using 6-benzylaminopurin (BAP) in the concentration of 1.0 mg/L for shadberry varieties' clonal micropropagation and indolebutyric acid (IBA) at 1.0 mg/L concentration for microshoots' rooting.https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/30/e3sconf_farba2021_04005.pdf
spellingShingle Raeva-Bogoslovskaya Ekaterina N.
Molkanova Olga I.
Kryuchkova Viktoriya A.
Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
E3S Web of Conferences
title Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
title_full Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
title_fullStr Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
title_full_unstemmed Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
title_short Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
title_sort some aspects of clonal micropropagation of amelanchier medik genus representatives
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/30/e3sconf_farba2021_04005.pdf
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