Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran
Background: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing c...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2020-03-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | http://ijms.sums.ac.ir/article_45423_683880989dc9491e05c9c07ce9ce4ec9.pdf |
| _version_ | 1811327341927858176 |
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| author | Sepideh Abdoli Saghar Safamanesh Mahsa Khosrojerdi Amir Azimian |
| author_facet | Sepideh Abdoli Saghar Safamanesh Mahsa Khosrojerdi Amir Azimian |
| author_sort | Sepideh Abdoli |
| collection | DOAJ |
| description | Background: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran.<br />Methods: All cerebrospinal fluid (CFS) samples suspected for bacterial meningitis were analyzed. The samples were collected during 2014-2018 in the Laboratory of Imam Reza Hospital (Bojnurd, Iran). Due to the high rate of false-negative cultures, polymerase chain reaction (PCR) was used for the detection of lytA and psaA genes of S. pneumoniae. In addition, the modified Marimon’s PCR method was used for serotyping the bacteria. The data were analyzed using Pearson’s Chi-square test. <br />Results: Out of the 901 CSF samples, 106 cases tested positive for S. pneumoniae using the PCR method, while only 92 cases tested positive using the conventional methods. Based on the Marimon’s PCR method, serotypes 23F, 19F, 19A, 1, 14, and serogroup 6A/B were the most common types. Serogroups 18C, 15A/F, 15B/C, 9A/V, 7A/F, 11A/D/F, and 22A/F were also detected in isolates. Note that 2.8% of the samples were non-typable (NT).<br />Conclusion: The results showed that only 13 serotypes were responsible for all meningitis cases. Pneumococcal capsular vaccine-13 (PCV-13) is the preferred choice against common serotypes of S. pneumoniae in northeast Iran. <br />The abstract was presented in Iran’s 19th International Congress of Microbiology, as a poster and published in the congress abstracts book. |
| first_indexed | 2024-04-13T15:04:57Z |
| format | Article |
| id | doaj.art-e31c4a254e024943b5a6619fd7d697c4 |
| institution | Directory Open Access Journal |
| issn | 0253-0716 1735-3688 |
| language | English |
| last_indexed | 2024-04-13T15:04:57Z |
| publishDate | 2020-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Medical Sciences |
| spelling | doaj.art-e31c4a254e024943b5a6619fd7d697c42022-12-22T02:42:11ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882020-03-0145212513310.30476/ijms.2019.4542345423Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast IranSepideh Abdoli0Saghar Safamanesh1Mahsa Khosrojerdi2Amir Azimian3Department of Biotechnology and Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, IranDepartment of Pathobiology and Laboratory Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran;|Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, IranDepartment of Pediatrics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, IranDepartment of Pathobiology and Laboratory Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran;|Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, IranBackground: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran.<br />Methods: All cerebrospinal fluid (CFS) samples suspected for bacterial meningitis were analyzed. The samples were collected during 2014-2018 in the Laboratory of Imam Reza Hospital (Bojnurd, Iran). Due to the high rate of false-negative cultures, polymerase chain reaction (PCR) was used for the detection of lytA and psaA genes of S. pneumoniae. In addition, the modified Marimon’s PCR method was used for serotyping the bacteria. The data were analyzed using Pearson’s Chi-square test. <br />Results: Out of the 901 CSF samples, 106 cases tested positive for S. pneumoniae using the PCR method, while only 92 cases tested positive using the conventional methods. Based on the Marimon’s PCR method, serotypes 23F, 19F, 19A, 1, 14, and serogroup 6A/B were the most common types. Serogroups 18C, 15A/F, 15B/C, 9A/V, 7A/F, 11A/D/F, and 22A/F were also detected in isolates. Note that 2.8% of the samples were non-typable (NT).<br />Conclusion: The results showed that only 13 serotypes were responsible for all meningitis cases. Pneumococcal capsular vaccine-13 (PCV-13) is the preferred choice against common serotypes of S. pneumoniae in northeast Iran. <br />The abstract was presented in Iran’s 19th International Congress of Microbiology, as a poster and published in the congress abstracts book.http://ijms.sums.ac.ir/article_45423_683880989dc9491e05c9c07ce9ce4ec9.pdfstreptococcus pneumoniaeserogroupvaccinesmeningitis |
| spellingShingle | Sepideh Abdoli Saghar Safamanesh Mahsa Khosrojerdi Amir Azimian Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran Iranian Journal of Medical Sciences streptococcus pneumoniae serogroup vaccines meningitis |
| title | Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran |
| title_full | Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran |
| title_fullStr | Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran |
| title_full_unstemmed | Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran |
| title_short | Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran |
| title_sort | molecular detection and serotyping of streptococcus pneumoniae in children with suspected meningitis in northeast iran |
| topic | streptococcus pneumoniae serogroup vaccines meningitis |
| url | http://ijms.sums.ac.ir/article_45423_683880989dc9491e05c9c07ce9ce4ec9.pdf |
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