Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism
Kolor is a teinturier grape cultivar, that accumulates flavonoids in the skin and pulp. However, the concentrations and proportions of flavonoids in Kolor skin and pulp differ, suggesting tissue specificity in teinturier grapes. Light conditions significantly influence the evolution of flavonoids. M...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1356799/full |
| _version_ | 1827320408044470272 |
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| author | Huiqing Li Huiqing Li Xiaotong Gao Xiaotong Gao Yu Wang Haocheng Lu Haocheng Lu Mengbo Tian Mengbo Tian Changqing Duan Changqing Duan Jun Wang Jun Wang |
| author_facet | Huiqing Li Huiqing Li Xiaotong Gao Xiaotong Gao Yu Wang Haocheng Lu Haocheng Lu Mengbo Tian Mengbo Tian Changqing Duan Changqing Duan Jun Wang Jun Wang |
| author_sort | Huiqing Li |
| collection | DOAJ |
| description | Kolor is a teinturier grape cultivar, that accumulates flavonoids in the skin and pulp. However, the concentrations and proportions of flavonoids in Kolor skin and pulp differ, suggesting tissue specificity in teinturier grapes. Light conditions significantly influence the evolution of flavonoids. Moreover, studies on the mechanisms governing flavonoid accumulation in light response sensitivity of teinturier grapes are limited. In the three consecutive years of study, the exposure of Kolor clusters was altered by bagging from pre-veraison to harvest. QqQ/MS and RT‒qPCR wereused to determine the individual anthocyanin contents and the relative gene expression. There was a significant decrease in the total anthocyanins and flavonols in the Kolor berries, with flavonols showing greater sensitivity to bagging. Bagging did not exert a consistent impact on the flavan-3-ols in Kolor berries. The sensitivities of anthocyanins in Kolor skin and pulp differed under light exclusion conditions. The concentration of trihydroxy-substituted anthocyanins in the skin decreased, while the proportion of dihydroxy-substituted anthocyanins in the pulp significantly increased, but the anthocyanin concentration in the pulp did not change significantly after bagging. The contents of malvidins and quercetins in the skin, and myricetins and quercetins in the pulp, were significantly reduced after bagging. The expression of flavonoid synthesis genes in Kolor skin and pulp was tissue-specific. After bagging, UFGT expression increased in the pulp and decreased in the skin. In addition, LDOX, FLS-1, CHI-1, CHI-2, F3H-1, F3H-2, and MYB4a exhibited sensitive light responses in both the skin and pulp. This study offers new insights into the regulation of flavonoids in Kolor grapes under light exclusion conditions. |
| first_indexed | 2024-04-25T00:45:40Z |
| format | Article |
| id | doaj.art-e31c7e56d70246a581ad5ac31534b9d5 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-25T00:45:40Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e31c7e56d70246a581ad5ac31534b9d52024-03-12T04:57:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13567991356799Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolismHuiqing Li0Huiqing Li1Xiaotong Gao2Xiaotong Gao3Yu Wang4Haocheng Lu5Haocheng Lu6Mengbo Tian7Mengbo Tian8Changqing Duan9Changqing Duan10Jun Wang11Jun Wang12Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKey Laboratory of Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Engineering Research Center for High Value Utilization of Characteristic Agricultural Products, College of Tea and Food Science and Technology, Anhui Agricultural University, Hefei, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaKolor is a teinturier grape cultivar, that accumulates flavonoids in the skin and pulp. However, the concentrations and proportions of flavonoids in Kolor skin and pulp differ, suggesting tissue specificity in teinturier grapes. Light conditions significantly influence the evolution of flavonoids. Moreover, studies on the mechanisms governing flavonoid accumulation in light response sensitivity of teinturier grapes are limited. In the three consecutive years of study, the exposure of Kolor clusters was altered by bagging from pre-veraison to harvest. QqQ/MS and RT‒qPCR wereused to determine the individual anthocyanin contents and the relative gene expression. There was a significant decrease in the total anthocyanins and flavonols in the Kolor berries, with flavonols showing greater sensitivity to bagging. Bagging did not exert a consistent impact on the flavan-3-ols in Kolor berries. The sensitivities of anthocyanins in Kolor skin and pulp differed under light exclusion conditions. The concentration of trihydroxy-substituted anthocyanins in the skin decreased, while the proportion of dihydroxy-substituted anthocyanins in the pulp significantly increased, but the anthocyanin concentration in the pulp did not change significantly after bagging. The contents of malvidins and quercetins in the skin, and myricetins and quercetins in the pulp, were significantly reduced after bagging. The expression of flavonoid synthesis genes in Kolor skin and pulp was tissue-specific. After bagging, UFGT expression increased in the pulp and decreased in the skin. In addition, LDOX, FLS-1, CHI-1, CHI-2, F3H-1, F3H-2, and MYB4a exhibited sensitive light responses in both the skin and pulp. This study offers new insights into the regulation of flavonoids in Kolor grapes under light exclusion conditions.https://www.frontiersin.org/articles/10.3389/fpls.2024.1356799/fullmicroclimateanthocyaninsflavonolsskinpulplight |
| spellingShingle | Huiqing Li Huiqing Li Xiaotong Gao Xiaotong Gao Yu Wang Haocheng Lu Haocheng Lu Mengbo Tian Mengbo Tian Changqing Duan Changqing Duan Jun Wang Jun Wang Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism Frontiers in Plant Science microclimate anthocyanins flavonols skin pulp light |
| title | Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism |
| title_full | Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism |
| title_fullStr | Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism |
| title_full_unstemmed | Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism |
| title_short | Artificial shading of teinturier grape Kolor clusters reveals light-responsive biomarkers: focus on flavonoid profiles and metabolism |
| title_sort | artificial shading of teinturier grape kolor clusters reveals light responsive biomarkers focus on flavonoid profiles and metabolism |
| topic | microclimate anthocyanins flavonols skin pulp light |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1356799/full |
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