Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern
Abstract Background Artemisia annua is the major source for artemisinin production. The artemisinin content in A. annua is affected by different types of light especially the UV light. UVR8, a member of RCC1 gene family was found to be the UV-B receptor in plants. The gene structures, evolutionary h...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-11-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-023-09786-4 |
| _version_ | 1797577984774766592 |
|---|---|
| author | Jieting Chen Wenguang Wu Xiaoxia Ding Danchun Zhang Chunyan Dai Hengyu Pan Peiqi Shi Chanjuan Wu Jun Zhang Jianmin Zhao Baosheng Liao Xiaohui Qiu Zhihai Huang |
| author_facet | Jieting Chen Wenguang Wu Xiaoxia Ding Danchun Zhang Chunyan Dai Hengyu Pan Peiqi Shi Chanjuan Wu Jun Zhang Jianmin Zhao Baosheng Liao Xiaohui Qiu Zhihai Huang |
| author_sort | Jieting Chen |
| collection | DOAJ |
| description | Abstract Background Artemisia annua is the major source for artemisinin production. The artemisinin content in A. annua is affected by different types of light especially the UV light. UVR8, a member of RCC1 gene family was found to be the UV-B receptor in plants. The gene structures, evolutionary history and expression profile of UVR8 or RCC1 genes remain undiscovered in A. annua. Results Twenty-two RCC1 genes (AaRCC1) were identified in each haplotype genome of two diploid strains of A. annua, LQ-9 and HAN1. Varied gene structures and sequences among paralogs were observed. The divergence of most RCC1 genes occurred at 46.7 – 51 MYA which overlapped with species divergence of core Asteraceae during the Eocene, while no recent novel RCC1 members were found in A. annua genome. The number of RCC1 genes remained stable among eudicots and RCC1 genes underwent purifying selection. The expression profile of AaRCC1 is analogous to that of Arabidopsis thaliana (AtRCC1) when responding to environmental stress. Conclusions This study provided a comprehensive characterization of the AaRCC1 gene family and suggested that RCC1 genes were conserved in gene number, structures, constitution of amino acids and expression profiles among eudicots. |
| first_indexed | 2024-03-10T22:15:52Z |
| format | Article |
| id | doaj.art-e320a098906f45a49d42982e0d8d6a47 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-10T22:15:52Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-e320a098906f45a49d42982e0d8d6a472023-11-19T12:27:45ZengBMCBMC Genomics1471-21642023-11-012411910.1186/s12864-023-09786-4Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary patternJieting Chen0Wenguang Wu1Xiaoxia Ding2Danchun Zhang3Chunyan Dai4Hengyu Pan5Peiqi Shi6Chanjuan Wu7Jun Zhang8Jianmin Zhao9Baosheng Liao10Xiaohui Qiu11Zhihai Huang12Key Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineArtemisinin Research Center, China Academy of Chinese Medical SciencesKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineSunribio Co.LtdSunribio Co.LtdSunribio Co.LtdKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineKey Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration, the Second Clinical College, Guangzhou University of Chinese MedicineAbstract Background Artemisia annua is the major source for artemisinin production. The artemisinin content in A. annua is affected by different types of light especially the UV light. UVR8, a member of RCC1 gene family was found to be the UV-B receptor in plants. The gene structures, evolutionary history and expression profile of UVR8 or RCC1 genes remain undiscovered in A. annua. Results Twenty-two RCC1 genes (AaRCC1) were identified in each haplotype genome of two diploid strains of A. annua, LQ-9 and HAN1. Varied gene structures and sequences among paralogs were observed. The divergence of most RCC1 genes occurred at 46.7 – 51 MYA which overlapped with species divergence of core Asteraceae during the Eocene, while no recent novel RCC1 members were found in A. annua genome. The number of RCC1 genes remained stable among eudicots and RCC1 genes underwent purifying selection. The expression profile of AaRCC1 is analogous to that of Arabidopsis thaliana (AtRCC1) when responding to environmental stress. Conclusions This study provided a comprehensive characterization of the AaRCC1 gene family and suggested that RCC1 genes were conserved in gene number, structures, constitution of amino acids and expression profiles among eudicots.https://doi.org/10.1186/s12864-023-09786-4Artemisia annuaRCC1UVR8UV-BPurifying selection |
| spellingShingle | Jieting Chen Wenguang Wu Xiaoxia Ding Danchun Zhang Chunyan Dai Hengyu Pan Peiqi Shi Chanjuan Wu Jun Zhang Jianmin Zhao Baosheng Liao Xiaohui Qiu Zhihai Huang Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern BMC Genomics Artemisia annua RCC1 UVR8 UV-B Purifying selection |
| title | Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern |
| title_full | Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern |
| title_fullStr | Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern |
| title_full_unstemmed | Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern |
| title_short | Genome-wide characterization of regulator of chromosome condensation 1 (RCC1) gene family in Artemisia annua L. revealed a conservation evolutionary pattern |
| title_sort | genome wide characterization of regulator of chromosome condensation 1 rcc1 gene family in artemisia annua l revealed a conservation evolutionary pattern |
| topic | Artemisia annua RCC1 UVR8 UV-B Purifying selection |
| url | https://doi.org/10.1186/s12864-023-09786-4 |
| work_keys_str_mv | AT jietingchen genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT wenguangwu genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT xiaoxiading genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT danchunzhang genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT chunyandai genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT hengyupan genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT peiqishi genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT chanjuanwu genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT junzhang genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT jianminzhao genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT baoshengliao genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT xiaohuiqiu genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern AT zhihaihuang genomewidecharacterizationofregulatorofchromosomecondensation1rcc1genefamilyinartemisiaannualrevealedaconservationevolutionarypattern |