Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis
Background: Reverse transcription polymerase chain reaction (RT-PCR) is the current standard of reference in the diagnosis of SARS-CoV-2 infection. In outpatient clinical practice, nasopharyngeal swab RT-PCR testing is still the most common procedure. The purpose of this systematic review and meta-a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2022-01-01
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| Series: | Caspian Journal of Internal Medicine |
| Subjects: | |
| Online Access: | http://caspjim.com/article-1-2969-en.html |
| _version_ | 1811272531835879424 |
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| author | Marco Marando Adriana Tamburello Pietro Gianella Rebecca Taylor Enos Bernasconi Tanja Fusi-Schmidhauser |
| author_facet | Marco Marando Adriana Tamburello Pietro Gianella Rebecca Taylor Enos Bernasconi Tanja Fusi-Schmidhauser |
| author_sort | Marco Marando |
| collection | DOAJ |
| description | Background: Reverse transcription polymerase chain reaction (RT-PCR) is the current standard of reference in the diagnosis of SARS-CoV-2 infection. In outpatient clinical practice, nasopharyngeal swab RT-PCR testing is still the most common procedure. The purpose of this systematic review and meta-analysis was to evaluate the sensitivity of RT-PCR nasopharyngeal assays.
Methods: We searched three databases, including PubMed/MEDLINE, EMBASE, and Cochrane Library, using a comprehensive strategy. Studies investigating the sensitivity of SARS-CoV-2 RT-PCR nasopharyngeal assays in adults were included. Two reviewers extracted data and assessed trial quality independently. Pooled sensitivity and its confidence interval were computed using the meta package in R.
Results: Thirteen studies were found eligible for the inclusion in the systematic review. Out of these, 25 different sub-studies were identified and included in the meta-analysis, which reported the sensitivities of 25 different nasopharyngeal RT-PCR assays. Finally, the overall pooled sensitivity resulted 89% (95% CI, 85.4 to 91.8%).
Conclusion: Our study suggests that RT-PCR assays on nasopharyngeal specimens have a substantial sensitivity for diagnosing SARS-CoV-2 infection. |
| first_indexed | 2024-04-12T22:42:01Z |
| format | Article |
| id | doaj.art-e32351df5c36490eb1813442e31e685c |
| institution | Directory Open Access Journal |
| issn | 2008-6164 2008-6172 |
| language | English |
| last_indexed | 2024-04-12T22:42:01Z |
| publishDate | 2022-01-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Caspian Journal of Internal Medicine |
| spelling | doaj.art-e32351df5c36490eb1813442e31e685c2022-12-22T03:13:42ZengBabol University of Medical SciencesCaspian Journal of Internal Medicine2008-61642008-61722022-01-0113Covid 19 Supplement 2139147Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-AnalysisMarco Marando0Adriana Tamburello1Pietro Gianella2Rebecca Taylor3Enos Bernasconi4Tanja Fusi-Schmidhauser5 Internal Medicine Department, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale, Switzerland Internal Medicine Department, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale, Switzerland Internal Medicine Department, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale, Switzerland Senior Associate Statistics, Lancaster Medical School, Lancaster University, UK Division of Infectious Diseases, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale, Switzerland Internal Medicine Department, Ospedale Regionale di Lugano, Ente Ospedaliero Cantonale, Switzerland Background: Reverse transcription polymerase chain reaction (RT-PCR) is the current standard of reference in the diagnosis of SARS-CoV-2 infection. In outpatient clinical practice, nasopharyngeal swab RT-PCR testing is still the most common procedure. The purpose of this systematic review and meta-analysis was to evaluate the sensitivity of RT-PCR nasopharyngeal assays. Methods: We searched three databases, including PubMed/MEDLINE, EMBASE, and Cochrane Library, using a comprehensive strategy. Studies investigating the sensitivity of SARS-CoV-2 RT-PCR nasopharyngeal assays in adults were included. Two reviewers extracted data and assessed trial quality independently. Pooled sensitivity and its confidence interval were computed using the meta package in R. Results: Thirteen studies were found eligible for the inclusion in the systematic review. Out of these, 25 different sub-studies were identified and included in the meta-analysis, which reported the sensitivities of 25 different nasopharyngeal RT-PCR assays. Finally, the overall pooled sensitivity resulted 89% (95% CI, 85.4 to 91.8%). Conclusion: Our study suggests that RT-PCR assays on nasopharyngeal specimens have a substantial sensitivity for diagnosing SARS-CoV-2 infection.http://caspjim.com/article-1-2969-en.htmlnasopharyngeal swabssars-cov-2rt-pcr assaydiagnostic yield. |
| spellingShingle | Marco Marando Adriana Tamburello Pietro Gianella Rebecca Taylor Enos Bernasconi Tanja Fusi-Schmidhauser Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis Caspian Journal of Internal Medicine nasopharyngeal swabs sars-cov-2 rt-pcr assay diagnostic yield. |
| title | Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis |
| title_full | Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis |
| title_fullStr | Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis |
| title_full_unstemmed | Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis |
| title_short | Diagnostic sensitivity of RT-PCR assays on nasopharyngeal specimens for detection of SARS-CoV-2 infection: A Systematic Review and Meta-Analysis |
| title_sort | diagnostic sensitivity of rt pcr assays on nasopharyngeal specimens for detection of sars cov 2 infection a systematic review and meta analysis |
| topic | nasopharyngeal swabs sars-cov-2 rt-pcr assay diagnostic yield. |
| url | http://caspjim.com/article-1-2969-en.html |
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