Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789
Abstract Background 2,3-Butanediol (BD) is a largely used fossil-based platform chemical. The yield and productivity of bio-based BD fermentative production must be increased and cheaper substrates need to be identified, to make bio-based BD production more competitive. As BD bioproduction occurs un...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-018-1138-4 |
| _version_ | 1828811546844725248 |
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| author | Stefano Rebecchi Davide Pinelli Giulio Zanaroli Fabio Fava Dario Frascari |
| author_facet | Stefano Rebecchi Davide Pinelli Giulio Zanaroli Fabio Fava Dario Frascari |
| author_sort | Stefano Rebecchi |
| collection | DOAJ |
| description | Abstract Background 2,3-Butanediol (BD) is a largely used fossil-based platform chemical. The yield and productivity of bio-based BD fermentative production must be increased and cheaper substrates need to be identified, to make bio-based BD production more competitive. As BD bioproduction occurs under microaerobic conditions, a fine tuning and control of the oxygen transfer rate (OTR) is crucial to maximize BD yield and productivity. Very few studies on BD bioproduction focused on the use of non-pathogenic microorganisms and of byproducts as substrate. The goal of this work was to optimize BD bioproduction by the non-pathogenic strain Bacillus licheniformis ATCC9789 by (i) identifying the ranges of volumetric and biomass-specific OTR that maximize BD yield and productivity using standard sugar and protein sources, and (ii) performing a preliminary evaluation of the variation in process performances and cost resulting from the replacement of glucose with molasses, and beef extract/peptone with chicken meat and bone meal, a byproduct of the meat production industry. Results OTR optimization with an expensive, standard medium containing glucose, beef extract and peptone revealed that OTRs in the 7–15 mmol/L/h range lead to an optimal BD yield (0.43 ± 0.03 g/g) and productivity (0.91 ± 0.05 g/L/h). The corresponding optimal range of biomass-specific OTR was equal to 1.4–7.9 $${\text{mmol}}_{{{\text{O}}_{2} }} /{\text{g}}_{\text{CDW}} /{\text{h}}$$ mmolO2/gCDW/h , whereas the respiratory quotient ranged from 1.8 to 2.5. The switch to an agro-industrial byproduct-based medium containing chicken meat and bone meal and molasses led to a 50% decrease in both BD yield and productivity. A preliminary economic analysis indicated that the use of the byproduct-based medium can reduce by about 45% the BD production cost. Conclusions A procedure for OTR optimization was developed and implemented, leading to the identification of a range of biomass-specific OTR and respiratory quotient to be used for the scale-up and control of BD bioproduction by Bacillus licheniformis. The switch to a byproduct-based medium led to a relevant decrease in BD production cost. Further research is needed to optimize the process of BD bioproduction from the tested byproduct-based medium. |
| first_indexed | 2024-12-12T09:29:34Z |
| format | Article |
| id | doaj.art-e3242e503d834fac98a69deba61142fc |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T09:29:34Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-e3242e503d834fac98a69deba61142fc2022-12-22T00:28:55ZengBMCBiotechnology for Biofuels1754-68342018-05-0111111410.1186/s13068-018-1138-4Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789Stefano Rebecchi0Davide Pinelli1Giulio Zanaroli2Fabio Fava3Dario Frascari4Department of Civil, Chemical, Environmental and Materials Engineering, University of BolognaDepartment of Civil, Chemical, Environmental and Materials Engineering, University of BolognaDepartment of Civil, Chemical, Environmental and Materials Engineering, University of BolognaDepartment of Civil, Chemical, Environmental and Materials Engineering, University of BolognaDepartment of Civil, Chemical, Environmental and Materials Engineering, University of BolognaAbstract Background 2,3-Butanediol (BD) is a largely used fossil-based platform chemical. The yield and productivity of bio-based BD fermentative production must be increased and cheaper substrates need to be identified, to make bio-based BD production more competitive. As BD bioproduction occurs under microaerobic conditions, a fine tuning and control of the oxygen transfer rate (OTR) is crucial to maximize BD yield and productivity. Very few studies on BD bioproduction focused on the use of non-pathogenic microorganisms and of byproducts as substrate. The goal of this work was to optimize BD bioproduction by the non-pathogenic strain Bacillus licheniformis ATCC9789 by (i) identifying the ranges of volumetric and biomass-specific OTR that maximize BD yield and productivity using standard sugar and protein sources, and (ii) performing a preliminary evaluation of the variation in process performances and cost resulting from the replacement of glucose with molasses, and beef extract/peptone with chicken meat and bone meal, a byproduct of the meat production industry. Results OTR optimization with an expensive, standard medium containing glucose, beef extract and peptone revealed that OTRs in the 7–15 mmol/L/h range lead to an optimal BD yield (0.43 ± 0.03 g/g) and productivity (0.91 ± 0.05 g/L/h). The corresponding optimal range of biomass-specific OTR was equal to 1.4–7.9 $${\text{mmol}}_{{{\text{O}}_{2} }} /{\text{g}}_{\text{CDW}} /{\text{h}}$$ mmolO2/gCDW/h , whereas the respiratory quotient ranged from 1.8 to 2.5. The switch to an agro-industrial byproduct-based medium containing chicken meat and bone meal and molasses led to a 50% decrease in both BD yield and productivity. A preliminary economic analysis indicated that the use of the byproduct-based medium can reduce by about 45% the BD production cost. Conclusions A procedure for OTR optimization was developed and implemented, leading to the identification of a range of biomass-specific OTR and respiratory quotient to be used for the scale-up and control of BD bioproduction by Bacillus licheniformis. The switch to a byproduct-based medium led to a relevant decrease in BD production cost. Further research is needed to optimize the process of BD bioproduction from the tested byproduct-based medium.http://link.springer.com/article/10.1186/s13068-018-1138-42,3-ButanediolOxygen transfer rateBacillus licheniformisProcess optimizationAgro-industrial by-productsMicroaerobic bioproduction |
| spellingShingle | Stefano Rebecchi Davide Pinelli Giulio Zanaroli Fabio Fava Dario Frascari Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 Biotechnology for Biofuels 2,3-Butanediol Oxygen transfer rate Bacillus licheniformis Process optimization Agro-industrial by-products Microaerobic bioproduction |
| title | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 |
| title_full | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 |
| title_fullStr | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 |
| title_full_unstemmed | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 |
| title_short | Effect of oxygen mass transfer rate on the production of 2,3-butanediol from glucose and agro-industrial byproducts by Bacillus licheniformis ATCC9789 |
| title_sort | effect of oxygen mass transfer rate on the production of 2 3 butanediol from glucose and agro industrial byproducts by bacillus licheniformis atcc9789 |
| topic | 2,3-Butanediol Oxygen transfer rate Bacillus licheniformis Process optimization Agro-industrial by-products Microaerobic bioproduction |
| url | http://link.springer.com/article/10.1186/s13068-018-1138-4 |
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