Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa
ABSTRACT Background and Objectives: Nowadays, the prevalence of multidrug-resistant pathogens such as Pseudomonas aeruginosa is increasing worldwide. Many studies have been seeking new treatment strategies to treat infections caused by these microorganisms. Silver nanoparticles (AgNPs) alon...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2017-11-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-1037-en.html |
| _version_ | 1818997476964696064 |
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| author | Mina Parsa Malahat Ahmadi Habib Dastmalchi Aliasghar Tehrani |
| author_facet | Mina Parsa Malahat Ahmadi Habib Dastmalchi Aliasghar Tehrani |
| author_sort | Mina Parsa |
| collection | DOAJ |
| description | ABSTRACT
Background and Objectives: Nowadays, the prevalence of multidrug-resistant pathogens such as Pseudomonas aeruginosa is increasing worldwide. Many studies have been seeking new treatment strategies to treat infections caused by these microorganisms. Silver nanoparticles (AgNPs) along with L-arginine have significant antimicrobial effects and could be used as alternatives for ineffective drugs.
Methods: In this study, the antibacterial activity of AgNPs, L-arginine and various concentrations of AgNPs along with L-arginine (12.5 and 25 mg/ml) were investigated against P. aeruginosa PAO1 using the broth macrodilution method.
Results: Minimum inhibitory concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 15.6 μg/ml, 25 mg/ml, 1.9 μg/ml and 3.9 μg/ml, respectively. Minimum bactericidal concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 31.2 μg/ml, 50 mg/ml, 3.9 μg/ml and 7.8 μg/ml, respectively.
Conclusion: Our study suggests that AgNPs along with L-arginine can be used as an alternative antibacterial agent against P. aeruginosa, and might be useful for treatment of wound infections.
Keywords: Nanoparticles, Arginine, Anti-Bacterial Agents, Pseudomonas aeruginosa. |
| first_indexed | 2024-12-20T21:46:15Z |
| format | Article |
| id | doaj.art-e325a49dc52d4c2092e37e65c7604e5a |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-20T21:46:15Z |
| publishDate | 2017-11-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-e325a49dc52d4c2092e37e65c7604e5a2022-12-21T19:25:41ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492017-11-01116711Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosaMina Parsa0Malahat Ahmadi1Habib Dastmalchi2Aliasghar Tehrani3 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Professor of Bacteriology, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Department of Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran ABSTRACT Background and Objectives: Nowadays, the prevalence of multidrug-resistant pathogens such as Pseudomonas aeruginosa is increasing worldwide. Many studies have been seeking new treatment strategies to treat infections caused by these microorganisms. Silver nanoparticles (AgNPs) along with L-arginine have significant antimicrobial effects and could be used as alternatives for ineffective drugs. Methods: In this study, the antibacterial activity of AgNPs, L-arginine and various concentrations of AgNPs along with L-arginine (12.5 and 25 mg/ml) were investigated against P. aeruginosa PAO1 using the broth macrodilution method. Results: Minimum inhibitory concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 15.6 μg/ml, 25 mg/ml, 1.9 μg/ml and 3.9 μg/ml, respectively. Minimum bactericidal concentration of AgNPs, L-arginine and AgNPs combined with 25 and 12.5 mg/ml L-arginine was 31.2 μg/ml, 50 mg/ml, 3.9 μg/ml and 7.8 μg/ml, respectively. Conclusion: Our study suggests that AgNPs along with L-arginine can be used as an alternative antibacterial agent against P. aeruginosa, and might be useful for treatment of wound infections. Keywords: Nanoparticles, Arginine, Anti-Bacterial Agents, Pseudomonas aeruginosa.http://mlj.goums.ac.ir/article-1-1037-en.htmlnanoparticlesarginineanti-bacterial agentspseudomonas aeruginosa. |
| spellingShingle | Mina Parsa Malahat Ahmadi Habib Dastmalchi Aliasghar Tehrani Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa Medical Laboratory Journal nanoparticles arginine anti-bacterial agents pseudomonas aeruginosa. |
| title | Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa |
| title_full | Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa |
| title_fullStr | Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa |
| title_full_unstemmed | Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa |
| title_short | Antibacterial Effect of Silver Nanoparticles along with L-Arginine against P. aeruginosa |
| title_sort | antibacterial effect of silver nanoparticles along with l arginine against p aeruginosa |
| topic | nanoparticles arginine anti-bacterial agents pseudomonas aeruginosa. |
| url | http://mlj.goums.ac.ir/article-1-1037-en.html |
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