The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple
Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple MdNAC29 gene and investigated its role in regulating...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1173107/full |
| _version_ | 1797785614669578240 |
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| author | Sen Li Sen Li Sen Li Xiuli Jing Xiuli Jing Xiuli Jing Qiuping Tan Qiuping Tan Qiuping Tan Binbin Wen Binbin Wen Binbin Wen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Xiude Chen Xiude Chen Xiude Chen Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li |
| author_facet | Sen Li Sen Li Sen Li Xiuli Jing Xiuli Jing Xiuli Jing Qiuping Tan Qiuping Tan Qiuping Tan Binbin Wen Binbin Wen Binbin Wen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Xiude Chen Xiude Chen Xiude Chen Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li |
| author_sort | Sen Li |
| collection | DOAJ |
| description | Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple MdNAC29 gene and investigated its role in regulating drought tolerance. Subcellular localization experiments showed that MdNAC29 was localized to the nucleus and transcription was induced by the PEG treatment. Over-expression of MdNAC29 reduced drought tolerance in apple plants, calli, and tobacco, and exhibited higher relative conductivity, malondialdehyde (MDA) content, and lower chlorophyll content under drought stress. The transcriptomic analyses revealed that MdNAC29 reduced drought resistance by modulating the expression of photosynthesis and leaf senescence-related genes. The qRT-PCR results showed that overexpression of MdNAC29 repressed the expression of drought-resistance genes. Yeast one-hybrid and dual-luciferase assays demonstrated that MdNAC29 directly repressed MdDREB2A expression. Moreover, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdNAC29 interacted with the MdPP2-B10 (F-box protein), which responded to drought stress, and MdPP2-B10 enhanced the repressive effect of MdNAC29 on the transcriptional activity of the MdDREB2A. Taken together, our results indicate that MdNAC29 is a negative regulator of drought resistance, and provide a theoretical basis for further molecular mechanism research. |
| first_indexed | 2024-03-13T00:56:32Z |
| format | Article |
| id | doaj.art-e32611f60d2443f3b46d14ef28132322 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-13T00:56:32Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e32611f60d2443f3b46d14ef281323222023-07-06T21:17:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.11731071173107The NAC transcription factor MdNAC29 negatively regulates drought tolerance in appleSen Li0Sen Li1Sen Li2Xiuli Jing3Xiuli Jing4Xiuli Jing5Qiuping Tan6Qiuping Tan7Qiuping Tan8Binbin Wen9Binbin Wen10Binbin Wen11Xiling Fu12Xiling Fu13Xiling Fu14Dongmei Li15Dongmei Li16Dongmei Li17Xiude Chen18Xiude Chen19Xiude Chen20Wei Xiao21Wei Xiao22Wei Xiao23Ling Li24Ling Li25Ling Li26College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, ChinaShandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, ChinaDrought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple MdNAC29 gene and investigated its role in regulating drought tolerance. Subcellular localization experiments showed that MdNAC29 was localized to the nucleus and transcription was induced by the PEG treatment. Over-expression of MdNAC29 reduced drought tolerance in apple plants, calli, and tobacco, and exhibited higher relative conductivity, malondialdehyde (MDA) content, and lower chlorophyll content under drought stress. The transcriptomic analyses revealed that MdNAC29 reduced drought resistance by modulating the expression of photosynthesis and leaf senescence-related genes. The qRT-PCR results showed that overexpression of MdNAC29 repressed the expression of drought-resistance genes. Yeast one-hybrid and dual-luciferase assays demonstrated that MdNAC29 directly repressed MdDREB2A expression. Moreover, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdNAC29 interacted with the MdPP2-B10 (F-box protein), which responded to drought stress, and MdPP2-B10 enhanced the repressive effect of MdNAC29 on the transcriptional activity of the MdDREB2A. Taken together, our results indicate that MdNAC29 is a negative regulator of drought resistance, and provide a theoretical basis for further molecular mechanism research.https://www.frontiersin.org/articles/10.3389/fpls.2023.1173107/fullappleMdNAC29MdDREB2Adrought toleranceF-box proteinRNA-seq |
| spellingShingle | Sen Li Sen Li Sen Li Xiuli Jing Xiuli Jing Xiuli Jing Qiuping Tan Qiuping Tan Qiuping Tan Binbin Wen Binbin Wen Binbin Wen Xiling Fu Xiling Fu Xiling Fu Dongmei Li Dongmei Li Dongmei Li Xiude Chen Xiude Chen Xiude Chen Wei Xiao Wei Xiao Wei Xiao Ling Li Ling Li Ling Li The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple Frontiers in Plant Science apple MdNAC29 MdDREB2A drought tolerance F-box protein RNA-seq |
| title | The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple |
| title_full | The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple |
| title_fullStr | The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple |
| title_full_unstemmed | The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple |
| title_short | The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple |
| title_sort | nac transcription factor mdnac29 negatively regulates drought tolerance in apple |
| topic | apple MdNAC29 MdDREB2A drought tolerance F-box protein RNA-seq |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1173107/full |
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