Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts
Most dramatic examples of actin reorganization have been described during host-microbe interactions. Plasticity of actin is, in part, due to posttranslational modifications such as phosphorylation or ubiquitylation. Here, we show for the first time that actins found in root nodules of Phaseolus vulg...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1267 |
| _version_ | 1811264545279180800 |
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| author | Edgar Dantán-González Yvonne Rosenstein Carmen Quinto Federico Sánchez |
| author_facet | Edgar Dantán-González Yvonne Rosenstein Carmen Quinto Federico Sánchez |
| author_sort | Edgar Dantán-González |
| collection | DOAJ |
| description | Most dramatic examples of actin reorganization have been described during host-microbe interactions. Plasticity of actin is, in part, due to posttranslational modifications such as phosphorylation or ubiquitylation. Here, we show for the first time that actins found in root nodules of Phaseolus vulgaris are modified transiently during nodule development by monoubiquitylation. This finding was extended to root nodules of other legumes and to other plants infected with mycorrhiza or plant pathogens such as members of the genera Pseudomonas and Phytophthora. However, neither viral infections nor diverse stressful conditions (heat shock, wounding, or osmotic stress) induced this response. Additionally, this phenomenon was mimicked by the addition of a yeast elicitor or H2O2 to Phaseolus vulgaris suspension culture cells. This modification seems to provide increased stability of the microfilaments to proteolytic degradation and seems to be found in fractions in which the actin cytoskeleton is associated with membranes. All together, these data suggest that actin monoubiquitylation may be considered an effector mechanism of a general plant response against microbes. |
| first_indexed | 2024-04-12T20:06:17Z |
| format | Article |
| id | doaj.art-e32809f7717343dab7d984b8c95666a8 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:06:17Z |
| publishDate | 2001-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-e32809f7717343dab7d984b8c95666a82022-12-22T03:18:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-11-0114111267127310.1094/MPMI.2001.14.11.1267Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and SymbiontsEdgar Dantán-GonzálezYvonne RosensteinCarmen QuintoFederico SánchezMost dramatic examples of actin reorganization have been described during host-microbe interactions. Plasticity of actin is, in part, due to posttranslational modifications such as phosphorylation or ubiquitylation. Here, we show for the first time that actins found in root nodules of Phaseolus vulgaris are modified transiently during nodule development by monoubiquitylation. This finding was extended to root nodules of other legumes and to other plants infected with mycorrhiza or plant pathogens such as members of the genera Pseudomonas and Phytophthora. However, neither viral infections nor diverse stressful conditions (heat shock, wounding, or osmotic stress) induced this response. Additionally, this phenomenon was mimicked by the addition of a yeast elicitor or H2O2 to Phaseolus vulgaris suspension culture cells. This modification seems to provide increased stability of the microfilaments to proteolytic degradation and seems to be found in fractions in which the actin cytoskeleton is associated with membranes. All together, these data suggest that actin monoubiquitylation may be considered an effector mechanism of a general plant response against microbes.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1267cortical cytoskeletonmicrofilaments stabilizationoxidative burst |
| spellingShingle | Edgar Dantán-González Yvonne Rosenstein Carmen Quinto Federico Sánchez Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts Molecular Plant-Microbe Interactions cortical cytoskeleton microfilaments stabilization oxidative burst |
| title | Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts |
| title_full | Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts |
| title_fullStr | Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts |
| title_full_unstemmed | Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts |
| title_short | Actin Monoubiquitylation Is Induced in Plants in Response to Pathogens and Symbionts |
| title_sort | actin monoubiquitylation is induced in plants in response to pathogens and symbionts |
| topic | cortical cytoskeleton microfilaments stabilization oxidative burst |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.11.1267 |
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