Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms
Cell-based therapies have great promise in accelerating and improving burn wound healing. It is a growing need to scale their competence to meet the clinical demands. In this study, the bone marrow mesenchymal stem cells (BMSCs) and platelet-rich plasma (PRP) were tested on the repair of induced bur...
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| Format: | Article |
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Elsevier
2023-09-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023066604 |
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| author | Bakinam M.H. Tammam Ola A. Habotta Manal El-khadragy Ahmed E. Abdel Moneim Mohga S. Abdalla |
| author_facet | Bakinam M.H. Tammam Ola A. Habotta Manal El-khadragy Ahmed E. Abdel Moneim Mohga S. Abdalla |
| author_sort | Bakinam M.H. Tammam |
| collection | DOAJ |
| description | Cell-based therapies have great promise in accelerating and improving burn wound healing. It is a growing need to scale their competence to meet the clinical demands. In this study, the bone marrow mesenchymal stem cells (BMSCs) and platelet-rich plasma (PRP) were tested on the repair of induced burn wounds in a murine model. After the induction of thermal injury, rats were injected with BMSCs and/or PRP in the burn area. After 4 weeks of post-burn, our findings revealed that local treatment of burnt skin with BMSCs and/or PRP offered substantial outcomes when compared with the untreated group. Injected burn with BMSCs and/or PRP enhanced the wound contraction rate and decreased the burn area and period of epithelization. Significant increases in VEGF together with declines in MMP-9 and TGF-β1 were observed in burnt areas after being treated with BMSCs and/or PRP therapy that indicated improved angiogenesis, and re-epithelization. Furthermore, both MSCs and PRP modulated the burn's oxidative and inflammatory microenvironment as indicated by increases in SOD, CAT, and GSH besides declines in MDA, IL-6, TNF-α, NF-κB, NO, and iNOS. Notable increases in Bcl-2 levels and decreases in Cas-3 and Bax levels were recorded in burnt skin that received both agents concomitantly. Interestingly, the histopathological examination validates the healing power of BMSCs and/or PRP. Collectively, BMSCs and PRP have pioneered therapeutics candidates for clinical application in burn healing possibly via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms along with regulating angiogenesis and scar formation. |
| first_indexed | 2024-03-11T20:51:04Z |
| format | Article |
| id | doaj.art-e3294744c28746878da0b2d27e6c9613 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:51:04Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-e3294744c28746878da0b2d27e6c96132023-10-01T05:59:42ZengElsevierHeliyon2405-84402023-09-0199e19452Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanismsBakinam M.H. Tammam0Ola A. Habotta1Manal El-khadragy2Ahmed E. Abdel Moneim3Mohga S. Abdalla4Chemistry Department, Molecular biotechnology Division, Faculty of Science, Helwan University, Cairo, EgyptDepartment of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptBiology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaZoology and Entomology Department, Faculty of Science, Helwan University, Cairo, Egypt; Corresponding author.Chemistry Department, Molecular biotechnology Division, Faculty of Science, Helwan University, Cairo, EgyptCell-based therapies have great promise in accelerating and improving burn wound healing. It is a growing need to scale their competence to meet the clinical demands. In this study, the bone marrow mesenchymal stem cells (BMSCs) and platelet-rich plasma (PRP) were tested on the repair of induced burn wounds in a murine model. After the induction of thermal injury, rats were injected with BMSCs and/or PRP in the burn area. After 4 weeks of post-burn, our findings revealed that local treatment of burnt skin with BMSCs and/or PRP offered substantial outcomes when compared with the untreated group. Injected burn with BMSCs and/or PRP enhanced the wound contraction rate and decreased the burn area and period of epithelization. Significant increases in VEGF together with declines in MMP-9 and TGF-β1 were observed in burnt areas after being treated with BMSCs and/or PRP therapy that indicated improved angiogenesis, and re-epithelization. Furthermore, both MSCs and PRP modulated the burn's oxidative and inflammatory microenvironment as indicated by increases in SOD, CAT, and GSH besides declines in MDA, IL-6, TNF-α, NF-κB, NO, and iNOS. Notable increases in Bcl-2 levels and decreases in Cas-3 and Bax levels were recorded in burnt skin that received both agents concomitantly. Interestingly, the histopathological examination validates the healing power of BMSCs and/or PRP. Collectively, BMSCs and PRP have pioneered therapeutics candidates for clinical application in burn healing possibly via antioxidant, anti-inflammatory, and anti-apoptotic mechanisms along with regulating angiogenesis and scar formation.http://www.sciencedirect.com/science/article/pii/S2405844023066604AngiogenesisBurn healingMesenchymal stem cellsOxidative stress and inflammationPlatelet-rich plasmaRe-epithelialization |
| spellingShingle | Bakinam M.H. Tammam Ola A. Habotta Manal El-khadragy Ahmed E. Abdel Moneim Mohga S. Abdalla Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms Heliyon Angiogenesis Burn healing Mesenchymal stem cells Oxidative stress and inflammation Platelet-rich plasma Re-epithelialization |
| title | Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms |
| title_full | Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms |
| title_fullStr | Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms |
| title_full_unstemmed | Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms |
| title_short | Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms |
| title_sort | therapeutic role of mesenchymal stem cells and platelet rich plasma on skin burn healing and rejuvenation a focus on scar regulation oxido inflammatory stress and apoptotic mechanisms |
| topic | Angiogenesis Burn healing Mesenchymal stem cells Oxidative stress and inflammation Platelet-rich plasma Re-epithelialization |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023066604 |
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