Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador
Anthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm with a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/6/378 |
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| author | Pamela Vinueza Marlon Calispa Luis Condolo Paula Toalombo Peter Geldhof |
| author_facet | Pamela Vinueza Marlon Calispa Luis Condolo Paula Toalombo Peter Geldhof |
| author_sort | Pamela Vinueza |
| collection | DOAJ |
| description | Anthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm with a known history of broad-spectrum anthelmintic usage in Ecuador. FBZ efficacy was analyzed using a fecal egg count reduction test (FECR test) and β-tubulin 1 mutation identification in <i>Cooperia</i> spp., the dominant nematode parasite identified before and after treatment. According to the FECR test, the nematode population was susceptible to FBZ. After amplifying and cloning the β-tubulin 1 of <i>Cooperia</i> spp., an F200Y mutation was found in 43% of the pooled larva coproculture after treatment. This study reports, for the first time, the presence of F200Y resistance-conferring mutation in <i>Cooperia</i> spp. in Ecuador. Although the nematode population was phenotypically susceptible to FBZ, the presence of F200Y suggests the existence of resistance in the early stages. Our findings highlight the need to implement alternative control strategies for parasitic infections besides broad-spectrum anthelmintics. |
| first_indexed | 2024-03-11T01:51:32Z |
| format | Article |
| id | doaj.art-e329723d7871429e8646310dbb112f5b |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T01:51:32Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-e329723d7871429e8646310dbb112f5b2023-11-18T13:00:03ZengMDPI AGVeterinary Sciences2306-73812023-05-0110637810.3390/vetsci10060378Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in EcuadorPamela Vinueza0Marlon Calispa1Luis Condolo2Paula Toalombo3Peter Geldhof4Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, EcuadorUCLouvain, Earth al Life Institute ELIE, ELI Croix du Sud 2L/L7.05.05, 1348 Louvain-la-Neuve, BelgiumEscuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, EcuadorEscuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, EcuadorLaboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133-B, 9820 Merelbeke, BelgiumAnthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm with a known history of broad-spectrum anthelmintic usage in Ecuador. FBZ efficacy was analyzed using a fecal egg count reduction test (FECR test) and β-tubulin 1 mutation identification in <i>Cooperia</i> spp., the dominant nematode parasite identified before and after treatment. According to the FECR test, the nematode population was susceptible to FBZ. After amplifying and cloning the β-tubulin 1 of <i>Cooperia</i> spp., an F200Y mutation was found in 43% of the pooled larva coproculture after treatment. This study reports, for the first time, the presence of F200Y resistance-conferring mutation in <i>Cooperia</i> spp. in Ecuador. Although the nematode population was phenotypically susceptible to FBZ, the presence of F200Y suggests the existence of resistance in the early stages. Our findings highlight the need to implement alternative control strategies for parasitic infections besides broad-spectrum anthelmintics.https://www.mdpi.com/2306-7381/10/6/378anthelminticmolecularbenzimidazole<i>Cooperia</i> spp.Ecuador |
| spellingShingle | Pamela Vinueza Marlon Calispa Luis Condolo Paula Toalombo Peter Geldhof Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador Veterinary Sciences anthelmintic molecular benzimidazole <i>Cooperia</i> spp. Ecuador |
| title | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador |
| title_full | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador |
| title_fullStr | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador |
| title_full_unstemmed | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador |
| title_short | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in <i>Cooperia</i> in Ecuador |
| title_sort | benzimidazole resistance in cattle the first report of the presence of f200y mutation in i cooperia i in ecuador |
| topic | anthelmintic molecular benzimidazole <i>Cooperia</i> spp. Ecuador |
| url | https://www.mdpi.com/2306-7381/10/6/378 |
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