Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines
Katarzyna Niemirowicz-Laskowska,1 Katarzyna Głuszek,1 Ewelina Piktel,1 Karlis Pajuste,2 Bonita Durnaś,3 Grzegorz Król,3 Agnieszka Z Wilczewska,4 Paul A Janmey,5 Aiva Plotniece,2 Robert Bucki1 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-06-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/bactericidal-and-immunomodulatory-properties-of-magnetic-nanoparticles-peer-reviewed-article-IJN |
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| author | Niemirowicz-Laskowska K Głuszek K Piktel E Pajuste K Durnaś B Król G Wilczewska AZ Janmey PA Plotniece A Bucki R |
| author_facet | Niemirowicz-Laskowska K Głuszek K Piktel E Pajuste K Durnaś B Król G Wilczewska AZ Janmey PA Plotniece A Bucki R |
| author_sort | Niemirowicz-Laskowska K |
| collection | DOAJ |
| description | Katarzyna Niemirowicz-Laskowska,1 Katarzyna Głuszek,1 Ewelina Piktel,1 Karlis Pajuste,2 Bonita Durnaś,3 Grzegorz Król,3 Agnieszka Z Wilczewska,4 Paul A Janmey,5 Aiva Plotniece,2 Robert Bucki1 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, Poland; 2Laboratory of Membrane Active Compounds and β-Diketones, Latvian Institute of Organic Synthesis, Riga, Latvia; 3Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce, 4Institute of Chemistry, University of Bialystok, Białystok, Poland; 5Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA Background: 1,4-Dihydropyridine (1,4-DHP) and its derivatives are well-known calcium channel blockers with antiarrhythmic and antihypertensive activities. These compounds exhibit pleiotropic effects including antimicrobial activities that rely on their positive charge and amphipathic nature. Use of magnetic nanoparticles (MNPs) as carriers of 1,4-DHP modulates their properties and enables improved formulations with higher efficacy and less toxicity. Methods: In this study, the antimicrobial and immunomodulatory activities of novel 1,4-DHP derivatives in free form and immobilized on MNPs were determined by evaluating pathogen outgrowth and proinflammatory cytokine release in experimental settings that involve incubation of various 1,4-DHPs with clinical isolates of bacteria or fungi as well as mammalian cell culture models. Results: Conventional immobilization of 1,4-DHP on aminosilane-coated MNPs markedly enhances their antimicrobial activity compared to nonimmobilized molecules, in part because of the higher affinity of these nanosystems for bacterial cell wall components in the presence of human body fluids. Conclusion: Optimized nanosystems are characterized by improved biocompatibility and higher anti-inflammatory properties that provide new opportunities for the therapy of infectious diseases. Keywords: magnetic nanoparticles, 1,4-dihydropyridine, antibacterial, antifungal, immunomodulatory properties |
| first_indexed | 2024-12-21T10:06:29Z |
| format | Article |
| id | doaj.art-e32e7d4c62804fc99358142898c368ef |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-21T10:06:29Z |
| publishDate | 2018-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-e32e7d4c62804fc99358142898c368ef2022-12-21T19:07:49ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-06-01Volume 133411342438759Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridinesNiemirowicz-Laskowska KGłuszek KPiktel EPajuste KDurnaś BKról GWilczewska AZJanmey PAPlotniece ABucki RKatarzyna Niemirowicz-Laskowska,1 Katarzyna Głuszek,1 Ewelina Piktel,1 Karlis Pajuste,2 Bonita Durnaś,3 Grzegorz Król,3 Agnieszka Z Wilczewska,4 Paul A Janmey,5 Aiva Plotniece,2 Robert Bucki1 1Department of Microbiological and Nanobiomedical Engineering, Medical University of Bialystok, Bialystok, Poland; 2Laboratory of Membrane Active Compounds and β-Diketones, Latvian Institute of Organic Synthesis, Riga, Latvia; 3Department of Microbiology and Immunology, The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce, 4Institute of Chemistry, University of Bialystok, Białystok, Poland; 5Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USA Background: 1,4-Dihydropyridine (1,4-DHP) and its derivatives are well-known calcium channel blockers with antiarrhythmic and antihypertensive activities. These compounds exhibit pleiotropic effects including antimicrobial activities that rely on their positive charge and amphipathic nature. Use of magnetic nanoparticles (MNPs) as carriers of 1,4-DHP modulates their properties and enables improved formulations with higher efficacy and less toxicity. Methods: In this study, the antimicrobial and immunomodulatory activities of novel 1,4-DHP derivatives in free form and immobilized on MNPs were determined by evaluating pathogen outgrowth and proinflammatory cytokine release in experimental settings that involve incubation of various 1,4-DHPs with clinical isolates of bacteria or fungi as well as mammalian cell culture models. Results: Conventional immobilization of 1,4-DHP on aminosilane-coated MNPs markedly enhances their antimicrobial activity compared to nonimmobilized molecules, in part because of the higher affinity of these nanosystems for bacterial cell wall components in the presence of human body fluids. Conclusion: Optimized nanosystems are characterized by improved biocompatibility and higher anti-inflammatory properties that provide new opportunities for the therapy of infectious diseases. Keywords: magnetic nanoparticles, 1,4-dihydropyridine, antibacterial, antifungal, immunomodulatory propertieshttps://www.dovepress.com/bactericidal-and-immunomodulatory-properties-of-magnetic-nanoparticles-peer-reviewed-article-IJNmagnetic nanoparticles14-dihydropyridineantibacterialantifungalimmunomodulatory properties |
| spellingShingle | Niemirowicz-Laskowska K Głuszek K Piktel E Pajuste K Durnaś B Król G Wilczewska AZ Janmey PA Plotniece A Bucki R Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines International Journal of Nanomedicine magnetic nanoparticles 1 4-dihydropyridine antibacterial antifungal immunomodulatory properties |
| title | Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines |
| title_full | Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines |
| title_fullStr | Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines |
| title_full_unstemmed | Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines |
| title_short | Bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1,4-dihydropyridines |
| title_sort | bactericidal and immunomodulatory properties of magnetic nanoparticles functionalized by 1 4 dihydropyridines |
| topic | magnetic nanoparticles 1 4-dihydropyridine antibacterial antifungal immunomodulatory properties |
| url | https://www.dovepress.com/bactericidal-and-immunomodulatory-properties-of-magnetic-nanoparticles-peer-reviewed-article-IJN |
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