IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.)
The seeds of two papaya (Carica papaya L.) cultivars ('Rainbow' and 'Sunrise Solo') were germinated on Murashige and Skoog (MS) medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be int...
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| Format: | Article |
| Language: | English |
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Alexandru Ioan Cuza University of Iasi
2013-12-01
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| Series: | Journal of Plant Development |
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| Online Access: | http://www.plant-journal.uaic.ro/docs/2013/6.pdf |
| _version_ | 1818207775712346112 |
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| author | Jaime A. TEIXEIRA DA SILVA |
| author_facet | Jaime A. TEIXEIRA DA SILVA |
| author_sort | Jaime A. TEIXEIRA DA SILVA |
| collection | DOAJ |
| description | The seeds of two papaya (Carica papaya L.) cultivars ('Rainbow' and 'Sunrise Solo') were germinated on Murashige and Skoog (MS) medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be interest in the in vitro mass production of papaya tissue of uniform origin. The most obvious form would be through the induction of somatic embryos, but rhizogenesis, an unexplored method, could provide as-yet unknown advantages. In this study, with the objective of artificaially inducing rhizogenesis in vitro, young leaves of both cultivars were placed on MS basal medium exposed to 5 concentrations (0, 1, 2, 4 or 8 mg/l) of auxins (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; α-naphthaleneacetic acid, NAA; β-naphthoxyacetic acid, BNOA) or phloroglucinol. All auxins could induce adventitious roots. Most roots (23/explant) formed with 2 mg/l NAA. The ability to induce only roots without any other intermediary organs such as callus or shoots provides an exclusive system for possible root-specific secondary metabolite production without the need for transgenic technologies such as Agrobacterium rhizogenes, or could provide a model protocol for more in-depth developmental studies on root development in papaya, an unexplored topic for this tropical plant. |
| first_indexed | 2024-12-12T04:34:17Z |
| format | Article |
| id | doaj.art-e32f27dea50a47f596658269ef875b62 |
| institution | Directory Open Access Journal |
| issn | 2065-3158 2066-9917 |
| language | English |
| last_indexed | 2024-12-12T04:34:17Z |
| publishDate | 2013-12-01 |
| publisher | Alexandru Ioan Cuza University of Iasi |
| record_format | Article |
| series | Journal of Plant Development |
| spelling | doaj.art-e32f27dea50a47f596658269ef875b622022-12-22T00:38:01ZengAlexandru Ioan Cuza University of IasiJournal of Plant Development2065-31582066-99172013-12-012015155IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.)Jaime A. TEIXEIRA DA SILVA0Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795 – JapanThe seeds of two papaya (Carica papaya L.) cultivars ('Rainbow' and 'Sunrise Solo') were germinated on Murashige and Skoog (MS) medium with 3% sucrose, and free of plant growth regulators. Papaya contains some important secondary metabolites such as papain, and there would be interest in the in vitro mass production of papaya tissue of uniform origin. The most obvious form would be through the induction of somatic embryos, but rhizogenesis, an unexplored method, could provide as-yet unknown advantages. In this study, with the objective of artificaially inducing rhizogenesis in vitro, young leaves of both cultivars were placed on MS basal medium exposed to 5 concentrations (0, 1, 2, 4 or 8 mg/l) of auxins (2,4,5-trichlorophenoxyacetic acid, 2,4,5-T; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; α-naphthaleneacetic acid, NAA; β-naphthoxyacetic acid, BNOA) or phloroglucinol. All auxins could induce adventitious roots. Most roots (23/explant) formed with 2 mg/l NAA. The ability to induce only roots without any other intermediary organs such as callus or shoots provides an exclusive system for possible root-specific secondary metabolite production without the need for transgenic technologies such as Agrobacterium rhizogenes, or could provide a model protocol for more in-depth developmental studies on root development in papaya, an unexplored topic for this tropical plant.http://www.plant-journal.uaic.ro/docs/2013/6.pdfleafMurashige and Skoogpaw-pawroots |
| spellingShingle | Jaime A. TEIXEIRA DA SILVA IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) Journal of Plant Development leaf Murashige and Skoog paw-paw roots |
| title | IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) |
| title_full | IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) |
| title_fullStr | IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) |
| title_full_unstemmed | IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) |
| title_short | IN VITRO RHIZOGENESIS IN PAPAYA (CARICA PAPAYA L.) |
| title_sort | in vitro rhizogenesis in papaya carica papaya l |
| topic | leaf Murashige and Skoog paw-paw roots |
| url | http://www.plant-journal.uaic.ro/docs/2013/6.pdf |
| work_keys_str_mv | AT jaimeateixeiradasilva invitrorhizogenesisinpapayacaricapapayal |