Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts
Background: Microbial oils produced by diverse microorganisms are being considered as alternative sources of triglycerides for biodiesel production. However, the standalone production of biodiesel from microorganisms is not currently economically feasible. In case of yeasts, the use of low-value nut...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-03-01
|
| Series: | Electronic Journal of Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345818300496 |
| _version_ | 1817978585262063616 |
|---|---|
| author | Adalgisa Martinez-Silveira Romina Villarreal Gabriela Garmendia Caterina Rufo Silvana Vero |
| author_facet | Adalgisa Martinez-Silveira Romina Villarreal Gabriela Garmendia Caterina Rufo Silvana Vero |
| author_sort | Adalgisa Martinez-Silveira |
| collection | DOAJ |
| description | Background: Microbial oils produced by diverse microorganisms are being considered as alternative sources of triglycerides for biodiesel production. However, the standalone production of biodiesel from microorganisms is not currently economically feasible. In case of yeasts, the use of low-value nutrient sources in microbial production and the implementation of cost-efficient downstream processes could reduce costs and make microbial lipids competitive with other commodity-type oils in biodiesel production. Industrial biodiesel synthesis from oleaginous seeds is currently based on a multistep process. However, a simple process called in situ transesterification (ISTE), which takes place within the biomass without a previous lipid extraction step, is receiving increasing interest. In this work, the optimal conditions for an ISTE process to obtain biodiesel from previously selected oleaginous yeast (Rhodotorula graminis S1/S2) were defined using the response surface methodology (RSM). Results: Using the RSM approach, the optimal conditions for the maximum yield with minimum reaction time included a methanol-to-biomass ratio of 60:1, 0.4 M H2SO4, and incubation at 70°C for 3 h. The optimized in situ process yield was significantly higher (123%) than that obtained with a two-step method in which fatty acids from saponifiable lipids were first extracted and then esterified with methanol. The composition of the fatty acid methyl ester mixture obtained from R. graminis S1/S2 by ISTE met Uruguayan standards for biodiesel. Conclusion: The characteristics achieved by the optimized method make microbial oil a potential alternative for biodiesel production from yeast at an industrial scale.How to cite: Martinez-Silveira A, Villarreal R, Garmendia G, et al. Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts. Electron J Biotechnol 2018;37. https://doi.org/10.1016/j.ejbt.2018.11.006. Keywords: Biodiesel, Biofuel, Environment, in situ transesterification, Methyl esters, Oleaginous microorganisms, Oleaginous yeast, Renewable energy, Response surface methodology, Rhodotorula graminis, Sustainable energy |
| first_indexed | 2024-04-13T22:31:01Z |
| format | Article |
| id | doaj.art-e331a17a0f8e4977b43e473d9dec3e41 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-13T22:31:01Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-e331a17a0f8e4977b43e473d9dec3e412022-12-22T02:26:55ZengElsevierElectronic Journal of Biotechnology0717-34582019-03-013819Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeastsAdalgisa Martinez-Silveira0Romina Villarreal1Gabriela Garmendia2Caterina Rufo3Silvana Vero4Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, Montevideo, Uruguay; Corresponding author.Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, Montevideo, UruguayÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, Montevideo, UruguayInstituto Polo Tecnológico, Facultad de Química, Universidad de la República, By Pass Ruta 8 s/n, Pando, Canelones, UruguayÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, Montevideo, UruguayBackground: Microbial oils produced by diverse microorganisms are being considered as alternative sources of triglycerides for biodiesel production. However, the standalone production of biodiesel from microorganisms is not currently economically feasible. In case of yeasts, the use of low-value nutrient sources in microbial production and the implementation of cost-efficient downstream processes could reduce costs and make microbial lipids competitive with other commodity-type oils in biodiesel production. Industrial biodiesel synthesis from oleaginous seeds is currently based on a multistep process. However, a simple process called in situ transesterification (ISTE), which takes place within the biomass without a previous lipid extraction step, is receiving increasing interest. In this work, the optimal conditions for an ISTE process to obtain biodiesel from previously selected oleaginous yeast (Rhodotorula graminis S1/S2) were defined using the response surface methodology (RSM). Results: Using the RSM approach, the optimal conditions for the maximum yield with minimum reaction time included a methanol-to-biomass ratio of 60:1, 0.4 M H2SO4, and incubation at 70°C for 3 h. The optimized in situ process yield was significantly higher (123%) than that obtained with a two-step method in which fatty acids from saponifiable lipids were first extracted and then esterified with methanol. The composition of the fatty acid methyl ester mixture obtained from R. graminis S1/S2 by ISTE met Uruguayan standards for biodiesel. Conclusion: The characteristics achieved by the optimized method make microbial oil a potential alternative for biodiesel production from yeast at an industrial scale.How to cite: Martinez-Silveira A, Villarreal R, Garmendia G, et al. Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts. Electron J Biotechnol 2018;37. https://doi.org/10.1016/j.ejbt.2018.11.006. Keywords: Biodiesel, Biofuel, Environment, in situ transesterification, Methyl esters, Oleaginous microorganisms, Oleaginous yeast, Renewable energy, Response surface methodology, Rhodotorula graminis, Sustainable energyhttp://www.sciencedirect.com/science/article/pii/S0717345818300496 |
| spellingShingle | Adalgisa Martinez-Silveira Romina Villarreal Gabriela Garmendia Caterina Rufo Silvana Vero Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts Electronic Journal of Biotechnology |
| title | Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| title_full | Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| title_fullStr | Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| title_full_unstemmed | Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| title_short | Process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| title_sort | process conditions for a rapid in situ transesterification for biodiesel production from oleaginous yeasts |
| url | http://www.sciencedirect.com/science/article/pii/S0717345818300496 |
| work_keys_str_mv | AT adalgisamartinezsilveira processconditionsforarapidinsitutransesterificationforbiodieselproductionfromoleaginousyeasts AT rominavillarreal processconditionsforarapidinsitutransesterificationforbiodieselproductionfromoleaginousyeasts AT gabrielagarmendia processconditionsforarapidinsitutransesterificationforbiodieselproductionfromoleaginousyeasts AT caterinarufo processconditionsforarapidinsitutransesterificationforbiodieselproductionfromoleaginousyeasts AT silvanavero processconditionsforarapidinsitutransesterificationforbiodieselproductionfromoleaginousyeasts |