Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system.
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes play important roles in CO2 fixation and redox balancing in photosynthetic bacteria. In the present study, the kefir yeast Kluyveromyces marxianus 4G5 was used as host for the transformation of form I and form II RubisCO genes derived f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0247135 |
| _version_ | 1831674569190014976 |
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| author | Dung Minh Ha-Tran Rou-Yin Lai Trinh Thi My Nguyen Eugene Huang Shou-Chen Lo Chieh-Chen Huang |
| author_facet | Dung Minh Ha-Tran Rou-Yin Lai Trinh Thi My Nguyen Eugene Huang Shou-Chen Lo Chieh-Chen Huang |
| author_sort | Dung Minh Ha-Tran |
| collection | DOAJ |
| description | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes play important roles in CO2 fixation and redox balancing in photosynthetic bacteria. In the present study, the kefir yeast Kluyveromyces marxianus 4G5 was used as host for the transformation of form I and form II RubisCO genes derived from the nonsulfur purple bacterium Rhodopseudomonas palustris using the Promoter-based Gene Assembly and Simultaneous Overexpression (PGASO) method. Hungateiclostridium thermocellum ATCC 27405, a well-known bacterium for its efficient solubilization of recalcitrant lignocellulosic biomass, was used to degrade Napier grass and rice straw to generate soluble fermentable sugars. The resultant Napier grass and rice straw broths were used as growth media for the engineered K. marxianus. In the dual microbial system, H. thermocellum degraded the biomass feedstock to produce both C5 and C6 sugars. As the bacterium only used hexose sugars, the remaining pentose sugars could be metabolized by K. marxianus to produce ethanol. The transformant RubisCO K. marxianus strains grew well in hydrolyzed Napier grass and rice straw broths and produced bioethanol more efficiently than the wild type. Therefore, these engineered K. marxianus strains could be used with H. thermocellum in a bacterium-yeast coculture system for ethanol production directly from biomass feedstocks. |
| first_indexed | 2024-12-20T03:47:04Z |
| format | Article |
| id | doaj.art-e3341fa4fe9849bcb3920f71c05b7b60 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:47:04Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e3341fa4fe9849bcb3920f71c05b7b602022-12-21T19:54:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024713510.1371/journal.pone.0247135Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system.Dung Minh Ha-TranRou-Yin LaiTrinh Thi My NguyenEugene HuangShou-Chen LoChieh-Chen HuangRibulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) genes play important roles in CO2 fixation and redox balancing in photosynthetic bacteria. In the present study, the kefir yeast Kluyveromyces marxianus 4G5 was used as host for the transformation of form I and form II RubisCO genes derived from the nonsulfur purple bacterium Rhodopseudomonas palustris using the Promoter-based Gene Assembly and Simultaneous Overexpression (PGASO) method. Hungateiclostridium thermocellum ATCC 27405, a well-known bacterium for its efficient solubilization of recalcitrant lignocellulosic biomass, was used to degrade Napier grass and rice straw to generate soluble fermentable sugars. The resultant Napier grass and rice straw broths were used as growth media for the engineered K. marxianus. In the dual microbial system, H. thermocellum degraded the biomass feedstock to produce both C5 and C6 sugars. As the bacterium only used hexose sugars, the remaining pentose sugars could be metabolized by K. marxianus to produce ethanol. The transformant RubisCO K. marxianus strains grew well in hydrolyzed Napier grass and rice straw broths and produced bioethanol more efficiently than the wild type. Therefore, these engineered K. marxianus strains could be used with H. thermocellum in a bacterium-yeast coculture system for ethanol production directly from biomass feedstocks.https://doi.org/10.1371/journal.pone.0247135 |
| spellingShingle | Dung Minh Ha-Tran Rou-Yin Lai Trinh Thi My Nguyen Eugene Huang Shou-Chen Lo Chieh-Chen Huang Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. PLoS ONE |
| title | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. |
| title_full | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. |
| title_fullStr | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. |
| title_full_unstemmed | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. |
| title_short | Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system. |
| title_sort | construction of engineered rubisco kluyveromyces marxianus for a dual microbial bioethanol production system |
| url | https://doi.org/10.1371/journal.pone.0247135 |
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