Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi.
Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0218564 |
| _version_ | 1819144237951746048 |
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| author | Elena Heidenreich Robin Wördenweber Frank Kirschhöfer Michael Nusser Frank Friedrich Kirsten Fahl Olaf Kruse Björn Rost Matthias Franzreb Gerald Brenner-Weiß Sebastian Rokitta |
| author_facet | Elena Heidenreich Robin Wördenweber Frank Kirschhöfer Michael Nusser Frank Friedrich Kirsten Fahl Olaf Kruse Björn Rost Matthias Franzreb Gerald Brenner-Weiß Sebastian Rokitta |
| author_sort | Elena Heidenreich |
| collection | DOAJ |
| description | Owing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi´s biomass composition. |
| first_indexed | 2024-12-22T12:38:57Z |
| format | Article |
| id | doaj.art-e3343d433cf84e7f8e6af43af331838e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T12:38:57Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e3343d433cf84e7f8e6af43af331838e2022-12-21T18:25:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01147e021856410.1371/journal.pone.0218564Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi.Elena HeidenreichRobin WördenweberFrank KirschhöferMichael NusserFrank FriedrichKirsten FahlOlaf KruseBjörn RostMatthias FranzrebGerald Brenner-WeißSebastian RokittaOwing to the hierarchical organization of biology, from genomes over transcriptomes and proteomes down to metabolomes, there is continuous debate about the extent to which data and interpretations derived from one level, e.g. the transcriptome, are in agreement with other levels, e.g. the metabolome. Here, we tested the effect of ocean acidification (OA; 400 vs. 1000 μatm CO2) and its modulation by light intensity (50 vs. 300 μmol photons m-2 s-1) on the biomass composition (represented by 75 key metabolites) of diploid and haploid life-cycle stages of the coccolithophore Emiliania huxleyi (RCC1216 and RCC1217) and compared these data with interpretations from previous physiological and gene expression screenings. The metabolite patterns showed minor responses to OA in both life-cycle stages. Whereas previous gene expression analyses suggested that the observed increased biomass buildup derived from lipid and carbohydrate storage, this dataset suggests that OA slightly increases overall biomass of cells, but does not significantly alter their metabolite composition. Generally, light was shown to be a more dominant driver of metabolite composition than OA, increasing the relative abundances of amino acids, mannitol and storage lipids, and shifting pigment contents to accommodate increased irradiance levels. The diploid stage was shown to contain vastly more osmolytes and mannitol than the haploid stage, which in turn had a higher relative content of amino acids, especially aromatic ones. Besides the differences between the investigated cell types and the general effects on biomass buildup, our analyses indicate that OA imposes only negligible effects on E. huxleyi´s biomass composition.https://doi.org/10.1371/journal.pone.0218564 |
| spellingShingle | Elena Heidenreich Robin Wördenweber Frank Kirschhöfer Michael Nusser Frank Friedrich Kirsten Fahl Olaf Kruse Björn Rost Matthias Franzreb Gerald Brenner-Weiß Sebastian Rokitta Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. PLoS ONE |
| title | Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. |
| title_full | Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. |
| title_fullStr | Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. |
| title_full_unstemmed | Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. |
| title_short | Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi. |
| title_sort | ocean acidification has little effect on the biochemical composition of the coccolithophore emiliania huxleyi |
| url | https://doi.org/10.1371/journal.pone.0218564 |
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