Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies
I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized. At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF fro...
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| Format: | Article |
| Language: | English |
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Hitit University
2019-03-01
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| Series: | Hittite Journal of Science and Engineering |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1506602 |
| _version_ | 1797662681460637696 |
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| author | Serap Durkut |
| author_facet | Serap Durkut |
| author_sort | Serap Durkut |
| collection | DOAJ |
| description | I n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized. At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 . The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period. In vitro study showed that PNVCL-g-Col was basically histocompatible. Then, the in vivo angiogenic activity of the VEGF-releasing PNVCL-g-Col was investigated using a subcutaneous rat model. In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Col |
| first_indexed | 2024-03-11T19:03:50Z |
| format | Article |
| id | doaj.art-e3355a9119c64e69bece07642546b640 |
| institution | Directory Open Access Journal |
| issn | 2148-4171 |
| language | English |
| last_indexed | 2024-03-11T19:03:50Z |
| publishDate | 2019-03-01 |
| publisher | Hitit University |
| record_format | Article |
| series | Hittite Journal of Science and Engineering |
| spelling | doaj.art-e3355a9119c64e69bece07642546b6402023-10-10T11:17:28ZengHitit UniversityHittite Journal of Science and Engineering2148-41712019-03-0161576110.17350/HJSE19030000121150Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo StudiesSerap Durkut0Ankara University, Tissue Engineering, Biomaterials and Nanobiotechnology Laboratory, Ankara, TurkeyI n this study, a thermosensitive poly N-vinylcaprolactam -g-collagen PNVCL-g-Col hybrid hydrogel was synthesized by conjugation using the NHS/EDC cross-linking system, and characterized. At first, the efficiency of in vitro sustained delivery of human vascular endothelial growth factor VEGF from the thermosensitive PNVCL-g-Col hydrogel modified with heparin, was evaluated for duration of ten days under in vitro physiological conditions 37oC, pH 7.4 . The results indicated that PNVCL-g-Col hydrogel preserved its stability and released ~90% of the loaded VEGF within this time period. In vitro study showed that PNVCL-g-Col was basically histocompatible. Then, the in vivo angiogenic activity of the VEGF-releasing PNVCL-g-Col was investigated using a subcutaneous rat model. In vivo study confirmed that angiogenic-factor-loaded PNVCL-g-Col had the capacity to induce neovascularization indicating that the in vivo bioactivity of the VEGF was preserved in the thermosensitive PNVCL-g-Colhttps://dergipark.org.tr/tr/download/article-file/1506602thermosensitive polymersustained releaseconjugationangiogenesishybrid hydrogelvegf. |
| spellingShingle | Serap Durkut Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies Hittite Journal of Science and Engineering thermosensitive polymer sustained release conjugation angiogenesis hybrid hydrogel vegf. |
| title | Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies |
| title_full | Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies |
| title_fullStr | Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies |
| title_full_unstemmed | Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies |
| title_short | Evaluation of Angiogenic Factor Release From Thermosensitive Poly N-Vinylcaprolactam -g-Collagen: In Vitro and In Vivo Studies |
| title_sort | evaluation of angiogenic factor release from thermosensitive poly n vinylcaprolactam g collagen in vitro and in vivo studies |
| topic | thermosensitive polymer sustained release conjugation angiogenesis hybrid hydrogel vegf. |
| url | https://dergipark.org.tr/tr/download/article-file/1506602 |
| work_keys_str_mv | AT serapdurkut evaluationofangiogenicfactorreleasefromthermosensitivepolynvinylcaprolactamgcollageninvitroandinvivostudies |