Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico
Cistus albidus: L., also known as Grey-leaved rockrose and locally addressed as šṭab or tûzzâla lbîḍa, is a plant species with a well-established reputation for its health-promoting properties and traditional use for the treatment of various diseases. This research delves into exploring the essentia...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2024.1334028/full |
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| author | Amine Elbouzidi Amine Elbouzidi Mohamed Taibi Mohamed Taibi Salah Laaraj El Hassania Loukili Mounir Haddou Naoufal El Hachlafi Hanae Naceiri Mrabti Abdellah Baraich Reda Bellaouchi Abdeslam Asehraou Mohammed Bourhia Hiba-Allah Nafidi Yousef A. Bin Jardan Khalid Chaabane Mohamed Addi |
| author_facet | Amine Elbouzidi Amine Elbouzidi Mohamed Taibi Mohamed Taibi Salah Laaraj El Hassania Loukili Mounir Haddou Naoufal El Hachlafi Hanae Naceiri Mrabti Abdellah Baraich Reda Bellaouchi Abdeslam Asehraou Mohammed Bourhia Hiba-Allah Nafidi Yousef A. Bin Jardan Khalid Chaabane Mohamed Addi |
| author_sort | Amine Elbouzidi |
| collection | DOAJ |
| description | Cistus albidus: L., also known as Grey-leaved rockrose and locally addressed as šṭab or tûzzâla lbîḍa, is a plant species with a well-established reputation for its health-promoting properties and traditional use for the treatment of various diseases. This research delves into exploring the essential oil extracted from the aerial components of Cistus albidus (referred to as CAEO), aiming to comprehend its properties concerning antioxidation, anti-inflammation, antimicrobial efficacy, and cytotoxicity. Firstly, a comprehensive analysis of CAEO’s chemical composition was performed through Gas Chromatography-Mass Spectrometry (GC-MS). Subsequently, four complementary assays were conducted to assess its antioxidant potential, including DPPH scavenging, β-carotene bleaching, ABTS scavenging, and total antioxidant capacity assays. The investigation delved into the anti-inflammatory properties via the 5-lipoxygenase assay and the antimicrobial effects of CAEO against various bacterial and fungal strains. Additionally, the research investigated the cytotoxic effects of CAEO on two human breast cancer subtypes, namely, MCF-7 and MDA-MB-231. Chemical analysis revealed camphene as the major compound, comprising 39.21% of the composition, followed by α-pinene (19.01%), bornyl acetate (18.32%), tricyclene (6.86%), and melonal (5.44%). Notably, CAEO exhibited robust antioxidant activity, as demonstrated by the low IC50 values in DPPH (153.92 ± 4.30 μg/mL) and β-carotene (95.25 ± 3.75 μg/mL) assays, indicating its ability to counteract oxidative damage. The ABTS assay and the total antioxidant capacity assay also confirmed the potent antioxidant potential with IC50 values of 120.51 ± 3.33 TE μmol/mL and 458.25 ± 3.67 µg AAE/mg, respectively. In terms of anti-inflammatory activity, CAEO displayed a substantial lipoxygenase inhibition at 0.5 mg/mL. Its antimicrobial properties were broad-spectrum, although some resistance was observed in the case of Escherichia coli and Staphylococcus aureus. CAEO exhibited significant dose-dependent inhibitory effects on tumor cell lines in vitro. Additionally, computational analyses were carried out to appraise the physicochemical characteristics, drug-likeness, and pharmacokinetic properties of CAEO’s constituent molecules, while the toxicity was assessed using the Protox II web server. |
| first_indexed | 2024-03-08T00:51:26Z |
| format | Article |
| id | doaj.art-e3370f7cfc7f460586da018846d5fb35 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-03-08T00:51:26Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-e3370f7cfc7f460586da018846d5fb352024-02-15T04:30:01ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462024-02-011210.3389/fchem.2024.13340281334028Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silicoAmine Elbouzidi0Amine Elbouzidi1Mohamed Taibi2Mohamed Taibi3Salah Laaraj4El Hassania Loukili5Mounir Haddou6Naoufal El Hachlafi7Hanae Naceiri Mrabti8Abdellah Baraich9Reda Bellaouchi10Abdeslam Asehraou11Mohammed Bourhia12Hiba-Allah Nafidi13Yousef A. Bin Jardan14Khalid Chaabane15Mohamed Addi16Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, MoroccoEuro-Mediterranean University of Fes (UEMF), Fes, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, MoroccoCentre de l’Oriental des Sciences et Technologies de l’Eau et de l’Environnement (COSTEE), Université Mohammed Premier, Oujda, MoroccoRegional Center of Agricultural Research of Tadla, National Institute of Agricultural Research (INRA), Rabat, MoroccoEuro-Mediterranean University of Fes (UEMF), Fes, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Faculty of Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, Fes, MoroccoHigh Institute of Nursing Professions and Health Techniques, Casablanca, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, MoroccoLaboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, MoroccoLaboratory of Biotechnology and Natural Resources Valorization, Faculty of Sciences of Agadir, Ibnou Zohr University, Agadir, MoroccoDepartment of Food Science, Faculty of Agricultural and Food Sciences, Laval University, Quebec City, QC, Canada0Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, MoroccoLaboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, MoroccoCistus albidus: L., also known as Grey-leaved rockrose and locally addressed as šṭab or tûzzâla lbîḍa, is a plant species with a well-established reputation for its health-promoting properties and traditional use for the treatment of various diseases. This research delves into exploring the essential oil extracted from the aerial components of Cistus albidus (referred to as CAEO), aiming to comprehend its properties concerning antioxidation, anti-inflammation, antimicrobial efficacy, and cytotoxicity. Firstly, a comprehensive analysis of CAEO’s chemical composition was performed through Gas Chromatography-Mass Spectrometry (GC-MS). Subsequently, four complementary assays were conducted to assess its antioxidant potential, including DPPH scavenging, β-carotene bleaching, ABTS scavenging, and total antioxidant capacity assays. The investigation delved into the anti-inflammatory properties via the 5-lipoxygenase assay and the antimicrobial effects of CAEO against various bacterial and fungal strains. Additionally, the research investigated the cytotoxic effects of CAEO on two human breast cancer subtypes, namely, MCF-7 and MDA-MB-231. Chemical analysis revealed camphene as the major compound, comprising 39.21% of the composition, followed by α-pinene (19.01%), bornyl acetate (18.32%), tricyclene (6.86%), and melonal (5.44%). Notably, CAEO exhibited robust antioxidant activity, as demonstrated by the low IC50 values in DPPH (153.92 ± 4.30 μg/mL) and β-carotene (95.25 ± 3.75 μg/mL) assays, indicating its ability to counteract oxidative damage. The ABTS assay and the total antioxidant capacity assay also confirmed the potent antioxidant potential with IC50 values of 120.51 ± 3.33 TE μmol/mL and 458.25 ± 3.67 µg AAE/mg, respectively. In terms of anti-inflammatory activity, CAEO displayed a substantial lipoxygenase inhibition at 0.5 mg/mL. Its antimicrobial properties were broad-spectrum, although some resistance was observed in the case of Escherichia coli and Staphylococcus aureus. CAEO exhibited significant dose-dependent inhibitory effects on tumor cell lines in vitro. Additionally, computational analyses were carried out to appraise the physicochemical characteristics, drug-likeness, and pharmacokinetic properties of CAEO’s constituent molecules, while the toxicity was assessed using the Protox II web server.https://www.frontiersin.org/articles/10.3389/fchem.2024.1334028/fullCistus albidus L.essential oilantioxidantantimicrobialanti-inflammatoryanticancer |
| spellingShingle | Amine Elbouzidi Amine Elbouzidi Mohamed Taibi Mohamed Taibi Salah Laaraj El Hassania Loukili Mounir Haddou Naoufal El Hachlafi Hanae Naceiri Mrabti Abdellah Baraich Reda Bellaouchi Abdeslam Asehraou Mohammed Bourhia Hiba-Allah Nafidi Yousef A. Bin Jardan Khalid Chaabane Mohamed Addi Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico Frontiers in Chemistry Cistus albidus L. essential oil antioxidant antimicrobial anti-inflammatory anticancer |
| title | Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico |
| title_full | Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico |
| title_fullStr | Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico |
| title_full_unstemmed | Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico |
| title_short | Chemical profiling of volatile compounds of the essential oil of grey-leaved rockrose (Cistus albidus L.) and its antioxidant, anti-inflammatory, antibacterial, antifungal, and anticancer activity in vitro and in silico |
| title_sort | chemical profiling of volatile compounds of the essential oil of grey leaved rockrose cistus albidus l and its antioxidant anti inflammatory antibacterial antifungal and anticancer activity in vitro and in silico |
| topic | Cistus albidus L. essential oil antioxidant antimicrobial anti-inflammatory anticancer |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2024.1334028/full |
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