Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity
The current study aimed to explore the possible prophylactic and therapeutic effect of Nigella sativa L. oil (NSO) against disruption of endocrine signals and injuries in the thyroid gland, ovary, and uterine tissues induced by sodium fluoride (NaF). Twenty-eight mature female Wistar rats were rando...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223018784 |
| _version_ | 1797364260462919680 |
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| author | Mona M. Elghareeb Gehad E. Elshopakey Shaymaa Rezk Ahmed Ateya Eman S. El-Ashry Mustafa Shukry Heba I. Ghamry Badriyah S. Alotaibi Nada M.A. Hashem |
| author_facet | Mona M. Elghareeb Gehad E. Elshopakey Shaymaa Rezk Ahmed Ateya Eman S. El-Ashry Mustafa Shukry Heba I. Ghamry Badriyah S. Alotaibi Nada M.A. Hashem |
| author_sort | Mona M. Elghareeb |
| collection | DOAJ |
| description | The current study aimed to explore the possible prophylactic and therapeutic effect of Nigella sativa L. oil (NSO) against disruption of endocrine signals and injuries in the thyroid gland, ovary, and uterine tissues induced by sodium fluoride (NaF). Twenty-eight mature female Wistar rats were randomly allocated into four experimental groups (n = 7/group) as follows: control group; NaF group, orally received NaF (20 mg/kg b.wt.) daily; NSO/NaF, orally received NSO (300 mg/kg b.wt.) two weeks before being given NaF and continued throughout the experiment; and NSO+NaF group orally received NSO concurrently with NaF. Our results indicated that NSO restored hormonal balance and suppressed oxidative damage and inflammation. Moreover, the levels of triiodothyronine, thyroxine, thyroid peroxidase, estrogen (E2), progesterone, follicle-stimulating hormone, and luteinizing hormone were elevated, while prostaglandins F2-α and cortisol levels were decreased in NSO treated groups compared to NaF-intoxicated rats. As well, NSO significantly boosted levels of antioxidant molecules, and lowered lipid peroxidation of examined tissues, unlike NaF-treated group. NSO also up-regulated antioxidant enzymes, anti-apoptotic protein, zona pellucida sperm-binding protein, bone morphogenetic protein, and thyroid stimulating hormone, conversely down-regulated inflammatory cytokines, apoptotic proteins, estrogen receptor-α, estrogen receptor-β, and thyroid stimulating hormone receptors compared to NaF-intoxicated group. Additionally, NSO ameliorated tissue damage of the thyroid gland, ovary, and uterus induced by NaF. -Overall, the prophylactic group (NSO/NaF) performed better antioxidant and anti-inflammatory activities than the treated group almost in all examined tissues, which is reflected by the improvement in the structure of the thyroid, ovarian, and uterine tissues. |
| first_indexed | 2024-03-08T16:33:44Z |
| format | Article |
| id | doaj.art-e3383571e7004c92be2d61d4949e8374 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-08T16:33:44Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-e3383571e7004c92be2d61d4949e83742024-01-06T04:38:13ZengElsevierBiomedicine & Pharmacotherapy0753-33222024-01-01170116080Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicityMona M. Elghareeb0Gehad E. Elshopakey1Shaymaa Rezk2Ahmed Ateya3Eman S. El-Ashry4Mustafa Shukry5Heba I. Ghamry6Badriyah S. Alotaibi7Nada M.A. Hashem8Department of Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt; Correspondence to: Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt; Correspondence to: Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.Nutrition and Food Science, Department of Home Economics, Faculty of Home Economics, King Khalid University, P.O. Box 960, Abha 61421, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptThe current study aimed to explore the possible prophylactic and therapeutic effect of Nigella sativa L. oil (NSO) against disruption of endocrine signals and injuries in the thyroid gland, ovary, and uterine tissues induced by sodium fluoride (NaF). Twenty-eight mature female Wistar rats were randomly allocated into four experimental groups (n = 7/group) as follows: control group; NaF group, orally received NaF (20 mg/kg b.wt.) daily; NSO/NaF, orally received NSO (300 mg/kg b.wt.) two weeks before being given NaF and continued throughout the experiment; and NSO+NaF group orally received NSO concurrently with NaF. Our results indicated that NSO restored hormonal balance and suppressed oxidative damage and inflammation. Moreover, the levels of triiodothyronine, thyroxine, thyroid peroxidase, estrogen (E2), progesterone, follicle-stimulating hormone, and luteinizing hormone were elevated, while prostaglandins F2-α and cortisol levels were decreased in NSO treated groups compared to NaF-intoxicated rats. As well, NSO significantly boosted levels of antioxidant molecules, and lowered lipid peroxidation of examined tissues, unlike NaF-treated group. NSO also up-regulated antioxidant enzymes, anti-apoptotic protein, zona pellucida sperm-binding protein, bone morphogenetic protein, and thyroid stimulating hormone, conversely down-regulated inflammatory cytokines, apoptotic proteins, estrogen receptor-α, estrogen receptor-β, and thyroid stimulating hormone receptors compared to NaF-intoxicated group. Additionally, NSO ameliorated tissue damage of the thyroid gland, ovary, and uterus induced by NaF. -Overall, the prophylactic group (NSO/NaF) performed better antioxidant and anti-inflammatory activities than the treated group almost in all examined tissues, which is reflected by the improvement in the structure of the thyroid, ovarian, and uterine tissues.http://www.sciencedirect.com/science/article/pii/S0753332223018784Nigella sativa oilSodium fluorideHormonal imbalanceOxidative damageInflammationOvarian/uterine dysfunction |
| spellingShingle | Mona M. Elghareeb Gehad E. Elshopakey Shaymaa Rezk Ahmed Ateya Eman S. El-Ashry Mustafa Shukry Heba I. Ghamry Badriyah S. Alotaibi Nada M.A. Hashem Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity Biomedicine & Pharmacotherapy Nigella sativa oil Sodium fluoride Hormonal imbalance Oxidative damage Inflammation Ovarian/uterine dysfunction |
| title | Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity |
| title_full | Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity |
| title_fullStr | Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity |
| title_full_unstemmed | Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity |
| title_short | Nigella sativa oil restores hormonal levels, and endocrine signals among thyroid, ovarian, and uterine tissues of female Wistar rats following sodium fluoride toxicity |
| title_sort | nigella sativa oil restores hormonal levels and endocrine signals among thyroid ovarian and uterine tissues of female wistar rats following sodium fluoride toxicity |
| topic | Nigella sativa oil Sodium fluoride Hormonal imbalance Oxidative damage Inflammation Ovarian/uterine dysfunction |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223018784 |
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