Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia
<b>AIM:</b> Toinvestigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2013-02-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580250 |
| _version_ | 1818158887517290496 |
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| author | Xiao-Dan Tian |
| author_facet | Xiao-Dan Tian |
| author_sort | Xiao-Dan Tian |
| collection | DOAJ |
| description | <b>AIM:</b> Toinvestigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression of type I collagen, α2 integrin and β1 integrin.<b>METHODS:</b> After 14 days of treatment, the refractive state and axial length were measured and the levels of type I collagen, α2 integrin and β1 integrin were assayed in the posterior sclerae of groups of guinea pigs that wore a monocular -7D polymethylmethacrylate (PMMA) lens or had -7D lens wear followed by the peribulbar injection of Phosphate Buffer Solution (PBS) or bFGF. The untreated fellow eye served as a control. Guinea pigs with no treatment served as normal group.<b>RESULTS:</b> The results showed that 14 days of monocular defocus increased axial eye length and refraction, while bFGF delivery inhibited them markedly. Further, it was also found that the monocular -7D lens could decrease the levels of type I collagen, α2 integrin and β1 integrin expressions, while, unlike PBS, bFGF increased them significantly in comparison to contralateral control eyes and normal eyes.<b>CONCLUSION:</b> bFGF can prevent the formation and development of defocus myopia by upregulating the expressions of type I collagen, α2 integrin and β1 integrin. Taken together, our results demonstrate that bFGF promotes sclera remodeling to prevent myopia in guinea pigs. |
| first_indexed | 2024-12-11T15:37:14Z |
| format | Article |
| id | doaj.art-e3397265949a4d1480e410db75370534 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-11T15:37:14Z |
| publishDate | 2013-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-e3397265949a4d1480e410db753705342022-12-22T00:59:55ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982013-02-0161545810.3980/j.issn.2222-3959.2013.01.11Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopiaXiao-Dan Tian<b>AIM:</b> Toinvestigate the expressions of type I collagen, α2 integrin and β1 integrin in the posterior sclera of guinea pigs with defocus myopia and whether basic fibroblast growth factor (bFGF) injection inhibits the formation and development of myopia by upregulating the expression of type I collagen, α2 integrin and β1 integrin.<b>METHODS:</b> After 14 days of treatment, the refractive state and axial length were measured and the levels of type I collagen, α2 integrin and β1 integrin were assayed in the posterior sclerae of groups of guinea pigs that wore a monocular -7D polymethylmethacrylate (PMMA) lens or had -7D lens wear followed by the peribulbar injection of Phosphate Buffer Solution (PBS) or bFGF. The untreated fellow eye served as a control. Guinea pigs with no treatment served as normal group.<b>RESULTS:</b> The results showed that 14 days of monocular defocus increased axial eye length and refraction, while bFGF delivery inhibited them markedly. Further, it was also found that the monocular -7D lens could decrease the levels of type I collagen, α2 integrin and β1 integrin expressions, while, unlike PBS, bFGF increased them significantly in comparison to contralateral control eyes and normal eyes.<b>CONCLUSION:</b> bFGF can prevent the formation and development of defocus myopia by upregulating the expressions of type I collagen, α2 integrin and β1 integrin. Taken together, our results demonstrate that bFGF promotes sclera remodeling to prevent myopia in guinea pigs.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580250defocus myopiatype I collagenα2 integrinβ1 integrinbFGF |
| spellingShingle | Xiao-Dan Tian Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia International Journal of Ophthalmology defocus myopia type I collagen α2 integrin β1 integrin bFGF |
| title | Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia |
| title_full | Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia |
| title_fullStr | Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia |
| title_full_unstemmed | Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia |
| title_short | Expressions of type I collagen, α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bFGF on the formation of myopia |
| title_sort | expressions of type i collagen α2 integrin and β1 integrin in sclera of guinea pig with defocus myopia and inhibitory effects of bfgf on the formation of myopia |
| topic | defocus myopia type I collagen α2 integrin β1 integrin bFGF |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3580250 |
| work_keys_str_mv | AT xiaodantian expressionsoftypeicollagena2integrinandb1integrininscleraofguineapigwithdefocusmyopiaandinhibitoryeffectsofbfgfontheformationofmyopia |