| Summary: | This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (<i>Sparus aurata</i> L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (<i>grp170</i>, <i>grp94</i>, <i>grp75</i>, <i>sod</i> and <i>hsp70</i>) and skin regeneration-related proteins (<i>tf</i>, <i>igf1</i>, <i>tgfb1</i>, <i>der1</i>, <i>apo1</i> and <i>erdj3</i>) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (<i>p</i> < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (<i>p</i> < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (<i>grp170</i> increased to 288 ± 85%, <i>grp94</i> to 502 ± 143%, <i>grp75</i> to 274 ± 69%, <i>sod</i> to 569 ± 99%, and <i>hsp70</i> increased to 537 ± 14%) (<i>p</i> < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish.
|
|---|