Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.)
This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (<i>Sparus aurata</i> L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (<i>grp170</i>, <i>grp94</i>, <i>gr...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/6/2/15 |
| _version_ | 1797537288392015872 |
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| author | Cristóbal Espinosa-Ruíz María Ángeles Esteban |
| author_facet | Cristóbal Espinosa-Ruíz María Ángeles Esteban |
| author_sort | Cristóbal Espinosa-Ruíz |
| collection | DOAJ |
| description | This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (<i>Sparus aurata</i> L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (<i>grp170</i>, <i>grp94</i>, <i>grp75</i>, <i>sod</i> and <i>hsp70</i>) and skin regeneration-related proteins (<i>tf</i>, <i>igf1</i>, <i>tgfb1</i>, <i>der1</i>, <i>apo1</i> and <i>erdj3</i>) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (<i>p</i> < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (<i>p</i> < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (<i>grp170</i> increased to 288 ± 85%, <i>grp94</i> to 502 ± 143%, <i>grp75</i> to 274 ± 69%, <i>sod</i> to 569 ± 99%, and <i>hsp70</i> increased to 537 ± 14%) (<i>p</i> < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish. |
| first_indexed | 2024-03-10T12:13:56Z |
| format | Article |
| id | doaj.art-e33e4cedbaea4f3a89bb7e6a41cfc254 |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-10T12:13:56Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj.art-e33e4cedbaea4f3a89bb7e6a41cfc2542023-11-21T16:03:12ZengMDPI AGFishes2410-38882021-04-01621510.3390/fishes6020015Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.)Cristóbal Espinosa-Ruíz0María Ángeles Esteban1Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, SpainImmunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100 Murcia, SpainThis study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (<i>Sparus aurata</i> L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (<i>grp170</i>, <i>grp94</i>, <i>grp75</i>, <i>sod</i> and <i>hsp70</i>) and skin regeneration-related proteins (<i>tf</i>, <i>igf1</i>, <i>tgfb1</i>, <i>der1</i>, <i>apo1</i> and <i>erdj3</i>) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (<i>p</i> < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (<i>p</i> < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (<i>grp170</i> increased to 288 ± 85%, <i>grp94</i> to 502 ± 143%, <i>grp75</i> to 274 ± 69%, <i>sod</i> to 569 ± 99%, and <i>hsp70</i> increased to 537 ± 14%) (<i>p</i> < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish.https://www.mdpi.com/2410-3888/6/2/15antioxidant enzymesfishgilthead seabream (<i>Sparus aurata</i> L.)mucosal immunityskin healingskin regeneration |
| spellingShingle | Cristóbal Espinosa-Ruíz María Ángeles Esteban Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) Fishes antioxidant enzymes fish gilthead seabream (<i>Sparus aurata</i> L.) mucosal immunity skin healing skin regeneration |
| title | Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) |
| title_full | Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) |
| title_fullStr | Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) |
| title_full_unstemmed | Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) |
| title_short | Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (<i>Sparus aurata</i> L.) |
| title_sort | wound induced changes in antioxidant enzyme activities in skin mucus and in gene expression in the skin of gilthead seabream i sparus aurata i l |
| topic | antioxidant enzymes fish gilthead seabream (<i>Sparus aurata</i> L.) mucosal immunity skin healing skin regeneration |
| url | https://www.mdpi.com/2410-3888/6/2/15 |
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