Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea

Bacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only i...

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Main Authors: Young Sun Lee, Gyoung Hee Kim, Young Jin Koh, Jae Sung Jung
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2021-10-01
Series:The Plant Pathology Journal
Subjects:
Online Access:http://www.ppjonline.org/upload/pdf/PPJ-NT-05-2021-0078.pdf
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author Young Sun Lee
Gyoung Hee Kim
Young Jin Koh
Jae Sung Jung
author_facet Young Sun Lee
Gyoung Hee Kim
Young Jin Koh
Jae Sung Jung
author_sort Young Sun Lee
collection DOAJ
description Bacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among P. syringe pv. actinidiae strains were determined by examining the presence of strA-strB or aadA, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the rpsL gene. All four streptomycin-resistant strains of P. syringe pv. actinidiae investigated in this study contained strA-strB as a resistant determinant. The presence of the aadA gene and a mutation in codon 43 of the rpsL gene was not identified.
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spelling doaj.art-e3416e58369c4effb1b338c45b80ce022022-12-22T03:44:50ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-10-0137548949310.5423/PPJ.NT.05.2021.00782275Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in KoreaYoung Sun Lee0Gyoung Hee Kim1Young Jin Koh2Jae Sung Jung3 Department of Biology, Sunchon National University, Suncheon 57922, Korea Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea Department of Plant Medicine, Sunchon National University, Suncheon 57922, Korea Department of Biology, Sunchon National University, Suncheon 57922, KoreaBacterial canker is a devastating disease of kiwifruit caused by the bacterium Pseudomonas syringe pv. actinidiae. Canker disease of kiwifruit in Korea has been controlled using streptomycin for more than two decades. Four streptomycin-resistant strains, belonging to biovar 2, which are found only in Korea, were collected between 2013 and 2014 from different orchards located in Jeju, Korea. The genetic background for streptomycin resistance among P. syringe pv. actinidiae strains were determined by examining the presence of strA-strB or aadA, which are genes frequently found in streptomycin-resistant bacteria, and a point mutation at codon 43 in the rpsL gene. All four streptomycin-resistant strains of P. syringe pv. actinidiae investigated in this study contained strA-strB as a resistant determinant. The presence of the aadA gene and a mutation in codon 43 of the rpsL gene was not identified.http://www.ppjonline.org/upload/pdf/PPJ-NT-05-2021-0078.pdfbacterial cankerkiwifruit pv. streptomycin resistance
spellingShingle Young Sun Lee
Gyoung Hee Kim
Young Jin Koh
Jae Sung Jung
Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
The Plant Pathology Journal
bacterial canker
kiwifruit
pv.
streptomycin resistance
title Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
title_full Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
title_fullStr Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
title_full_unstemmed Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
title_short Identification of Genes in Streptomycin-Resistant pv. Biovar 2 Strains Isolated in Korea
title_sort identification of genes in streptomycin resistant pv biovar 2 strains isolated in korea
topic bacterial canker
kiwifruit
pv.
streptomycin resistance
url http://www.ppjonline.org/upload/pdf/PPJ-NT-05-2021-0078.pdf
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AT gyoungheekim identificationofgenesinstreptomycinresistantpvbiovar2strainsisolatedinkorea
AT youngjinkoh identificationofgenesinstreptomycinresistantpvbiovar2strainsisolatedinkorea
AT jaesungjung identificationofgenesinstreptomycinresistantpvbiovar2strainsisolatedinkorea