Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S]
A subject's baseline FA composition may influence the ability of dietary highly unsaturated omega-3 FAs (n3-HUFA) to change circulating profiles of esterified FAs and their oxygenated metabolites. This study evaluates the influence of basal n3-HUFA and n3-oxylipin status on the magnitude of res...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-08-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752041870X |
| _version_ | 1818934487780687872 |
|---|---|
| author | Alison H. Keenan Theresa L. Pedersen Kristi Fillaus Mark K. Larson Gregory C. Shearer John W. Newman |
| author_facet | Alison H. Keenan Theresa L. Pedersen Kristi Fillaus Mark K. Larson Gregory C. Shearer John W. Newman |
| author_sort | Alison H. Keenan |
| collection | DOAJ |
| description | A subject's baseline FA composition may influence the ability of dietary highly unsaturated omega-3 FAs (n3-HUFA) to change circulating profiles of esterified FAs and their oxygenated metabolites. This study evaluates the influence of basal n3-HUFA and n3-oxylipin status on the magnitude of response to n3-HUFA consumption. Blood was collected from fasting subjects (n = 30) before and after treatment (4 weeks; 11 ± 2 mg/kg/day n3-HUFA ethyl esters). Esterified FAs were quantified in erythrocytes, platelets, and plasma by GC-MS. Esterified oxylipins were quantified in plasma by LC-MS/MS. Treatment with n3-HUFAs increased n3-HUFAs and decreased n6-HUFAs in all reservoirs and increased plasma n3-oxylipins without significantly changing n6-oxylipin concentrations. As subject basal n3-HUFAs increased, treatment-associated changes decreased, and this behavior was reflected in the percentage of 20:5n3 + 22:6n3 in red blood cell membrane FAs (i.e., the omega-3 index). To maintain an omega-3 index of 8% and thus reduce cardiovascular disease risk, our analyses suggest a maintenance dose of 7 mg/kg/day n3-HUFA ethyl esters for a 70-kg individual. These results suggest that the basal n3 index may have clinical utility to establish efficacious therapeutic experimental feeding regimens and to evaluate the USDA Dietary Guidelines recommendations for n3-HUFA consumption. |
| first_indexed | 2024-12-20T05:05:04Z |
| format | Article |
| id | doaj.art-e34368c8277f4faa92ad786941c954e0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T05:05:04Z |
| publishDate | 2012-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e34368c8277f4faa92ad786941c954e02022-12-21T19:52:25ZengElsevierJournal of Lipid Research0022-22752012-08-0153816621669Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S]Alison H. Keenan0Theresa L. Pedersen1Kristi Fillaus2Mark K. Larson3Gregory C. Shearer4John W. Newman5Department of Nutrition, University of California, Davis, CA, 95616USDA, ARS, Western Human Nutrition Research Center, Davis, CA, 95616Cardiovascular Health Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD, 57117Department of Biology, Augustana College, Sioux Falls, SD, 57197; andCardiovascular Health Research Center, Sanford Research/University of South Dakota, Sioux Falls, SD, 57117; Basic Biomedical Science & Internal Medicine, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, 57069To whom correspondence should be addressed. e-mail: john.newman@ars.usda.gov.; Department of Nutrition, University of California, Davis, CA, 95616; USDA, ARS, Western Human Nutrition Research Center, Davis, CA, 95616; To whom correspondence should be addressed. e-mail: john.newman@ars.usda.gov.A subject's baseline FA composition may influence the ability of dietary highly unsaturated omega-3 FAs (n3-HUFA) to change circulating profiles of esterified FAs and their oxygenated metabolites. This study evaluates the influence of basal n3-HUFA and n3-oxylipin status on the magnitude of response to n3-HUFA consumption. Blood was collected from fasting subjects (n = 30) before and after treatment (4 weeks; 11 ± 2 mg/kg/day n3-HUFA ethyl esters). Esterified FAs were quantified in erythrocytes, platelets, and plasma by GC-MS. Esterified oxylipins were quantified in plasma by LC-MS/MS. Treatment with n3-HUFAs increased n3-HUFAs and decreased n6-HUFAs in all reservoirs and increased plasma n3-oxylipins without significantly changing n6-oxylipin concentrations. As subject basal n3-HUFAs increased, treatment-associated changes decreased, and this behavior was reflected in the percentage of 20:5n3 + 22:6n3 in red blood cell membrane FAs (i.e., the omega-3 index). To maintain an omega-3 index of 8% and thus reduce cardiovascular disease risk, our analyses suggest a maintenance dose of 7 mg/kg/day n3-HUFA ethyl esters for a 70-kg individual. These results suggest that the basal n3 index may have clinical utility to establish efficacious therapeutic experimental feeding regimens and to evaluate the USDA Dietary Guidelines recommendations for n3-HUFA consumption.http://www.sciencedirect.com/science/article/pii/S002222752041870Xomega-3 fatty acidsoxylipinstargeted metabolomicsbaseline statushighly unsaturated fatty acid |
| spellingShingle | Alison H. Keenan Theresa L. Pedersen Kristi Fillaus Mark K. Larson Gregory C. Shearer John W. Newman Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] Journal of Lipid Research omega-3 fatty acids oxylipins targeted metabolomics baseline status highly unsaturated fatty acid |
| title | Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] |
| title_full | Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] |
| title_fullStr | Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] |
| title_full_unstemmed | Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] |
| title_short | Basal omega-3 fatty acid status affects fatty acid and oxylipin responses to high-dose n3-HUFA in healthy volunteers[S] |
| title_sort | basal omega 3 fatty acid status affects fatty acid and oxylipin responses to high dose n3 hufa in healthy volunteers s |
| topic | omega-3 fatty acids oxylipins targeted metabolomics baseline status highly unsaturated fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S002222752041870X |
| work_keys_str_mv | AT alisonhkeenan basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss AT theresalpedersen basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss AT kristifillaus basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss AT markklarson basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss AT gregorycshearer basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss AT johnwnewman basalomega3fattyacidstatusaffectsfattyacidandoxylipinresponsestohighdosen3hufainhealthyvolunteerss |