Modified technique of Wolbachia removal from Malaysian Aedes albopictus
Objective: To develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings. Methods: In this study, seven different tetracycline treatment met...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-07-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301283 |
| _version_ | 1798044402366545920 |
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| author | Sylvia Joanne Indra Vythilingam Nava Yugavathy Jonathan Inbaraj Doss |
| author_facet | Sylvia Joanne Indra Vythilingam Nava Yugavathy Jonathan Inbaraj Doss |
| author_sort | Sylvia Joanne |
| collection | DOAJ |
| description | Objective: To develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings.
Methods: In this study, seven different tetracycline treatment methods were conducted to obtain the best removal method. Four methods focused on larvae tetracycline treatment, one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment.
Results: All larval tetracycline treatments resulted in either high larvae mortality, sterile F0 adult mosquitoes or unsuccessful Wolbachia removal. Treatment of both larvae and adults resulted in reduced larvae mortality, successful Wolbachia removal but slow mosquito fecundity. As for the adult treatment, 1.0 mg/mL as previously published was not able to completely remove Wolbachia in F1 generation whereas 1.25 mg/mL successfully removed Wolbachia from F1 and F2 mosquitoes in 2 weeks.
Conclusions: This method is different from the previously published methods as it provides an improved Wolbachia removal technique from Ae. albopictus with high egg hatchability, low larvae mortality, increased fecundity and better Wolbachia removal rate. |
| first_indexed | 2024-04-11T23:03:11Z |
| format | Article |
| id | doaj.art-e344affb53854342a8407b425a9b348c |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-04-11T23:03:11Z |
| publishDate | 2014-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-e344affb53854342a8407b425a9b348c2022-12-22T03:58:04ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-07-014755756010.12980/APJTB.4.2014APJTB-2014-0020Modified technique of Wolbachia removal from Malaysian Aedes albopictusSylvia Joanne0Indra Vythilingam1Nava Yugavathy2Jonathan Inbaraj Doss3Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, MalaysiaDepartment of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, MalaysiaDepartment of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, MalaysiaJulius Centre University of Malaya, Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, MalaysiaObjective: To develop an artificial and modified Wolbachia removal technique using tetracycline from naturally Wolbachia infected Aedes albopictus (Ae. albopictus) so as to be able to produce generations of Wolbachia free offsprings. Methods: In this study, seven different tetracycline treatment methods were conducted to obtain the best removal method. Four methods focused on larvae tetracycline treatment, one method on both larvae and adult tetracycline treatment and the last two methods on adult mosquito sucrose treatment. Results: All larval tetracycline treatments resulted in either high larvae mortality, sterile F0 adult mosquitoes or unsuccessful Wolbachia removal. Treatment of both larvae and adults resulted in reduced larvae mortality, successful Wolbachia removal but slow mosquito fecundity. As for the adult treatment, 1.0 mg/mL as previously published was not able to completely remove Wolbachia in F1 generation whereas 1.25 mg/mL successfully removed Wolbachia from F1 and F2 mosquitoes in 2 weeks. Conclusions: This method is different from the previously published methods as it provides an improved Wolbachia removal technique from Ae. albopictus with high egg hatchability, low larvae mortality, increased fecundity and better Wolbachia removal rate.http://www.sciencedirect.com/science/article/pii/S2221169115301283WolbachiatetracyclineAedes albopictus |
| spellingShingle | Sylvia Joanne Indra Vythilingam Nava Yugavathy Jonathan Inbaraj Doss Modified technique of Wolbachia removal from Malaysian Aedes albopictus Asian Pacific Journal of Tropical Biomedicine Wolbachia tetracycline Aedes albopictus |
| title | Modified technique of Wolbachia removal from Malaysian Aedes albopictus |
| title_full | Modified technique of Wolbachia removal from Malaysian Aedes albopictus |
| title_fullStr | Modified technique of Wolbachia removal from Malaysian Aedes albopictus |
| title_full_unstemmed | Modified technique of Wolbachia removal from Malaysian Aedes albopictus |
| title_short | Modified technique of Wolbachia removal from Malaysian Aedes albopictus |
| title_sort | modified technique of wolbachia removal from malaysian aedes albopictus |
| topic | Wolbachia tetracycline Aedes albopictus |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301283 |
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