XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
Abstract Objective The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. Methods The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detec...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13578-018-0269-4 |
| _version_ | 1818110188065914880 |
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| author | Zhihua Xiao Yijun Wang Hao Ding |
| author_facet | Zhihua Xiao Yijun Wang Hao Ding |
| author_sort | Zhihua Xiao |
| collection | DOAJ |
| description | Abstract Objective The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. Methods The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p. Results In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B. Conclusions Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC. |
| first_indexed | 2024-12-11T02:43:10Z |
| format | Article |
| id | doaj.art-e3485f0a78044177b7a1e42c03fe511d |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-11T02:43:10Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-e3485f0a78044177b7a1e42c03fe511d2022-12-22T01:23:31ZengBMCCell & Bioscience2045-37012019-01-019111210.1186/s13578-018-0269-4XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCCZhihua Xiao0Yijun Wang1Hao Ding2Department of Gastroenterology, The Second Affiliated Hospital of Nanchang UniversityThe Second Clinical Medical College of Nanchang UniversityDepartment of Gastroenterology, The Second Affiliated Hospital of Nanchang UniversityAbstract Objective The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. Methods The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p. Results In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B. Conclusions Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.http://link.springer.com/article/10.1186/s13578-018-0269-4XPDMiR-29a-3pCell proliferation and migrationHepatocellular carcinoma |
| spellingShingle | Zhihua Xiao Yijun Wang Hao Ding XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC Cell & Bioscience XPD MiR-29a-3p Cell proliferation and migration Hepatocellular carcinoma |
| title | XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC |
| title_full | XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC |
| title_fullStr | XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC |
| title_full_unstemmed | XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC |
| title_short | XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC |
| title_sort | xpd suppresses cell proliferation and migration via mir 29a 3p mdm2 pdgf b axis in hcc |
| topic | XPD MiR-29a-3p Cell proliferation and migration Hepatocellular carcinoma |
| url | http://link.springer.com/article/10.1186/s13578-018-0269-4 |
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