Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease
Background: Coronary artery disease (CAD) is the most frequent cause of morbidity and mortality in the world and it is known as a multifactorial disorder which is influenced by both genetic and environmental factors. Based on different assays, the platelet derived growth factor B (PDGF-B) gene is sh...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-10-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110863017300411 |
| _version_ | 1811262877269491712 |
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| author | Shayesteh Rezayani Touraj Farazmandfar Majid shahbazi |
| author_facet | Shayesteh Rezayani Touraj Farazmandfar Majid shahbazi |
| author_sort | Shayesteh Rezayani |
| collection | DOAJ |
| description | Background: Coronary artery disease (CAD) is the most frequent cause of morbidity and mortality in the world and it is known as a multifactorial disorder which is influenced by both genetic and environmental factors. Based on different assays, the platelet derived growth factor B (PDGF-B) gene is shown to be amongst the inflammation inducers involved in different pathological conditions such as atherosclerosis. Aim: In this case-control study we have examined the association of the functional PDGF-B +286 and +1135 polymorphisms and its expression status with susceptibility to CAD. Subjects and methods: Study groups included 369 patients with CAD and 413 healthy individuals. Genotypic and allelic frequencies of PDGF-B +286 and +1135 polymorphisms were determined by the SSP-PCR method. PDGF-B expression status was identified by quantitative real-time PCR. Results: The analysis of genotyping results revealed CAD patients had an increased frequency of PDGF-B +286A>G A/A genotype in comparison with healthy individuals. Furthermore, we found that the PDGF-B expression level in CAD patients group is nearly 2-folds greater than its level in control group. Conclusion: There is probably a relationship between variations in PDGF-B gene and CAD influence. The increase in PDGF-B gene expression may has a role in susceptibility to CAD. Keywords: Coronary artery disease, PDGF-B, Polymorphism, Genotyping, mRNA expression |
| first_indexed | 2024-04-12T19:34:43Z |
| format | Article |
| id | doaj.art-e34becd308cc49b3aea724751abc062c |
| institution | Directory Open Access Journal |
| issn | 1110-8630 |
| language | English |
| last_indexed | 2024-04-12T19:34:43Z |
| publishDate | 2017-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-e34becd308cc49b3aea724751abc062c2022-12-22T03:19:15ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302017-10-01184359363Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery diseaseShayesteh Rezayani0Touraj Farazmandfar1Majid shahbazi2Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, IranMedical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, IranCorresponding author at: Department of Medical Genetics, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran; Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, IranBackground: Coronary artery disease (CAD) is the most frequent cause of morbidity and mortality in the world and it is known as a multifactorial disorder which is influenced by both genetic and environmental factors. Based on different assays, the platelet derived growth factor B (PDGF-B) gene is shown to be amongst the inflammation inducers involved in different pathological conditions such as atherosclerosis. Aim: In this case-control study we have examined the association of the functional PDGF-B +286 and +1135 polymorphisms and its expression status with susceptibility to CAD. Subjects and methods: Study groups included 369 patients with CAD and 413 healthy individuals. Genotypic and allelic frequencies of PDGF-B +286 and +1135 polymorphisms were determined by the SSP-PCR method. PDGF-B expression status was identified by quantitative real-time PCR. Results: The analysis of genotyping results revealed CAD patients had an increased frequency of PDGF-B +286A>G A/A genotype in comparison with healthy individuals. Furthermore, we found that the PDGF-B expression level in CAD patients group is nearly 2-folds greater than its level in control group. Conclusion: There is probably a relationship between variations in PDGF-B gene and CAD influence. The increase in PDGF-B gene expression may has a role in susceptibility to CAD. Keywords: Coronary artery disease, PDGF-B, Polymorphism, Genotyping, mRNA expressionhttp://www.sciencedirect.com/science/article/pii/S1110863017300411 |
| spellingShingle | Shayesteh Rezayani Touraj Farazmandfar Majid shahbazi Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease Egyptian Journal of Medical Human Genetics |
| title | Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease |
| title_full | Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease |
| title_fullStr | Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease |
| title_full_unstemmed | Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease |
| title_short | Association assessment of platelet derived growth factor B gene polymorphism and its expression status with susceptibility to coronary artery disease |
| title_sort | association assessment of platelet derived growth factor b gene polymorphism and its expression status with susceptibility to coronary artery disease |
| url | http://www.sciencedirect.com/science/article/pii/S1110863017300411 |
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