Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe.

Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe.

<h4>Background</h4>Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to c...

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Bibliographic Details
Main Authors: Ayako Chino, Kenji Watanabe, Hisao Moriya
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-03-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20300182/?tool=EBI

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