<i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin
Alkamides have been observed to interact in different ways in several superior organisms and have been used in traditional medicine in many countries e.g., to relieve pain. Previous studies showed that affinin when applied to other plant species induces prominent changes in the root architecture and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/3/143 |
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| author | Tonatiu Campos-García Jorge Molina-Torres |
| author_facet | Tonatiu Campos-García Jorge Molina-Torres |
| author_sort | Tonatiu Campos-García |
| collection | DOAJ |
| description | Alkamides have been observed to interact in different ways in several superior organisms and have been used in traditional medicine in many countries e.g., to relieve pain. Previous studies showed that affinin when applied to other plant species induces prominent changes in the root architecture and induces transcriptional adjustments; however, little is known about the metabolic pathways recruited by plants in response to alkamides. Previous published work with Arabidopsis seedlings treated in vitro with affinin at 50 µM significantly reduced primary root length. In tomato seedlings, that concentration did not reduce root growth but increase the number and length of lateral roots. Non-targeted metabolomic analysis by Gas Chromatography couplet to Mass Spectrometry (GC/EIMS) showed that, in tomato seedlings, affinin increased the accumulation of several metabolites leading to an enrichment of several metabolic pathways. Affinin at 100 µM alters the accumulation of metabolites such as organic acids, amino acids, sugars, and fatty acids. Finally, our results showed a response possibly associated with nitrogen, GABA shunt and serine pathways, in addition to a possible alteration in the mitochondrial electron transport chain (ETC), interesting topics to understand the molecular and metabolic mechanisms in response to alkamide in plants. |
| first_indexed | 2024-03-09T06:18:14Z |
| format | Article |
| id | doaj.art-e34d6ab1dfb148e48b18bd82bab29f7c |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T06:18:14Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-e34d6ab1dfb148e48b18bd82bab29f7c2023-12-03T11:51:14ZengMDPI AGMetabolites2218-19892021-02-0111314310.3390/metabo11030143<i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide AffininTonatiu Campos-García0Jorge Molina-Torres1Department of Biotecnología y Bioquímica, CINVESTAV Unidad Irapuato, Irapuato, Guanajuato 36924, MexicoDepartment of Biotecnología y Bioquímica, CINVESTAV Unidad Irapuato, Irapuato, Guanajuato 36924, MexicoAlkamides have been observed to interact in different ways in several superior organisms and have been used in traditional medicine in many countries e.g., to relieve pain. Previous studies showed that affinin when applied to other plant species induces prominent changes in the root architecture and induces transcriptional adjustments; however, little is known about the metabolic pathways recruited by plants in response to alkamides. Previous published work with Arabidopsis seedlings treated in vitro with affinin at 50 µM significantly reduced primary root length. In tomato seedlings, that concentration did not reduce root growth but increase the number and length of lateral roots. Non-targeted metabolomic analysis by Gas Chromatography couplet to Mass Spectrometry (GC/EIMS) showed that, in tomato seedlings, affinin increased the accumulation of several metabolites leading to an enrichment of several metabolic pathways. Affinin at 100 µM alters the accumulation of metabolites such as organic acids, amino acids, sugars, and fatty acids. Finally, our results showed a response possibly associated with nitrogen, GABA shunt and serine pathways, in addition to a possible alteration in the mitochondrial electron transport chain (ETC), interesting topics to understand the molecular and metabolic mechanisms in response to alkamide in plants.https://www.mdpi.com/2218-1989/11/3/143<i>Solanum lycopersicum</i>alkamidesaffininmetabolite profilingtomatoplant immunity |
| spellingShingle | Tonatiu Campos-García Jorge Molina-Torres <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin Metabolites <i>Solanum lycopersicum</i> alkamides affinin metabolite profiling tomato plant immunity |
| title | <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin |
| title_full | <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin |
| title_fullStr | <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin |
| title_full_unstemmed | <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin |
| title_short | <i>Solanum lycopersicum</i> Seedlings. Metabolic Responses Induced by the Alkamide Affinin |
| title_sort | i solanum lycopersicum i seedlings metabolic responses induced by the alkamide affinin |
| topic | <i>Solanum lycopersicum</i> alkamides affinin metabolite profiling tomato plant immunity |
| url | https://www.mdpi.com/2218-1989/11/3/143 |
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