Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor
An acetylcholinesterase (AchE) based amperometric biosensor was developed by immobilisation of the enzyme onto a self assembled modified gold electrode. Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2008-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/8/4600/ |
| _version_ | 1811184739751559168 |
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| author | Mauro Bertotti Sergio A. S. Machado Josiane Caetano Valber A. Pedrosa |
| author_facet | Mauro Bertotti Sergio A. S. Machado Josiane Caetano Valber A. Pedrosa |
| author_sort | Mauro Bertotti |
| collection | DOAJ |
| description | An acetylcholinesterase (AchE) based amperometric biosensor was developed by immobilisation of the enzyme onto a self assembled modified gold electrode. Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirmed the formation of thiocholine and its electrochemical oxidation at Ep = 0.28 V vs Ag/AgCl. An indirect methodology involving the inhibition effect of parathion and carbaryl on the enzymatic reaction was developed and employed to measure both pesticides in spiked natural water and food samples without pre-treatment or pre-concentration steps. Values higher than 91-98.0% in recovery experiments indicated the feasibility of the proposed electroanalytical methodology to quantify both pesticides in water or food samples. HPLC measurements were also performed for comparison and confirmed the values measured amperometrically. |
| first_indexed | 2024-04-11T13:17:32Z |
| format | Article |
| id | doaj.art-e351b806645b4ddf8da22ae56665c47a |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:17:32Z |
| publishDate | 2008-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-e351b806645b4ddf8da22ae56665c47a2022-12-22T04:22:20ZengMDPI AGSensors1424-82202008-08-018846004610Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical BiosensorMauro BertottiSergio A. S. MachadoJosiane CaetanoValber A. PedrosaAn acetylcholinesterase (AchE) based amperometric biosensor was developed by immobilisation of the enzyme onto a self assembled modified gold electrode. Cyclic voltammetric experiments performed with the SAM-AchE biosensor in phosphate buffer solutions (pH = 7.2) containing acetylthiocholine confirmed the formation of thiocholine and its electrochemical oxidation at Ep = 0.28 V vs Ag/AgCl. An indirect methodology involving the inhibition effect of parathion and carbaryl on the enzymatic reaction was developed and employed to measure both pesticides in spiked natural water and food samples without pre-treatment or pre-concentration steps. Values higher than 91-98.0% in recovery experiments indicated the feasibility of the proposed electroanalytical methodology to quantify both pesticides in water or food samples. HPLC measurements were also performed for comparison and confirmed the values measured amperometrically.http://www.mdpi.com/1424-8220/8/8/4600/BiosensorPesticidesAcetylcholinesteraseCreek WaterFood |
| spellingShingle | Mauro Bertotti Sergio A. S. Machado Josiane Caetano Valber A. Pedrosa Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor Sensors Biosensor Pesticides Acetylcholinesterase Creek Water Food |
| title | Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor |
| title_full | Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor |
| title_fullStr | Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor |
| title_full_unstemmed | Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor |
| title_short | Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer /Acetylcholinesterase Electrochemical Biosensor |
| title_sort | determination of parathion and carbaryl pesticides in water and food samples using a self assembled monolayer acetylcholinesterase electrochemical biosensor |
| topic | Biosensor Pesticides Acetylcholinesterase Creek Water Food |
| url | http://www.mdpi.com/1424-8220/8/8/4600/ |
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