Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks
The present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups...
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698213/full |
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| author | Yang Liu Yang Liu Qian Lin Xuan Huang Guitao Jiang Chuang Li Xu Zhang Shengli Liu Lingyun He Yali Liu Qiuzhong Dai Xingguo Huang |
| author_facet | Yang Liu Yang Liu Qian Lin Xuan Huang Guitao Jiang Chuang Li Xu Zhang Shengli Liu Lingyun He Yali Liu Qiuzhong Dai Xingguo Huang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | The present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups. Each group contained six replicates of 10 birds. The dietary treatments were corn-soybean-based diet supplemented with FA at the concentrations of 0 (control), 100, 200, 400, and 800 mg/kg diet. The results demonstrated that dietary FA at the levels of 200, 400, and 800 mg/kg increased the average daily gain (P = 0.01), 400 and 800 mg/kg FA increased the final body weight (P = 0.02), 100, 200, and 800 mg/kg FA increased the serum glutathione (P = 0.01), and 100, 400, and 800 mg/kg FA increased the glutathione peroxidase activities in birds (P < 0.01). Additionally, 200, 400, and 800 mg/kg dietary FA lowered the serum levels of interleukin-2 (P = 0.02) and interleukin-6 (P = 0.04). Moreover, the morphometric study of the intestines indicated that 400 mg/kg FA decreased the crypt depth in jejunum (P = 0.01) and caecum (P = 0.04), and increased the ratio of villus height to crypt depth in jejunum (P = 0.02). Significant linear and/or quadratic relationships were found between FA concentration and the measured parameters. 16S rRNA sequencing revealed that dietary FA increased the populations of genera Faecalibacterium, Paludicola, RF39, and Faecalicoccus in the cecum (P < 0.05), whereas decreased the populations of Anaerofilum and UCG-002 (P < 0.05). The Spearman correlation analysis indicated that phylum Proteobacteria were negatively, but order Oscillospirales, and family Ruminococcaceae were positively related to the parameters of the growth performance. Phylum Bacteroidetes, class Negativicutes and family Rikenellaceae were negatively associated with the parameters of the antioxidative capability. And phylum Cyanobacteria, Elusimicrobia, and Bacteroidetes, class Bacilli, family Rikenellaceae, and genus Prevotella were positively associated with the parameters of the immunological capability. Thus, it was concluded that the supplementations of 400 mg/kg FA in diet was able to improve the growth performance, antioxidative and immunological capabilities, intestinal morphology, and modulated the gut microbial construction of Linwu ducks at the growing stage. |
| first_indexed | 2024-12-22T13:38:54Z |
| format | Article |
| id | doaj.art-e35358119fde4715bbdbef4f7948e7c4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T13:38:54Z |
| publishDate | 2021-07-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-e35358119fde4715bbdbef4f7948e7c42022-12-21T18:23:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-07-011210.3389/fmicb.2021.698213698213Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in DucksYang Liu0Yang Liu1Qian Lin2Xuan Huang3Guitao Jiang4Chuang Li5Xu Zhang6Shengli Liu7Lingyun He8Yali Liu9Qiuzhong Dai10Xingguo Huang11College of Animal Science and Technology, Hunan Agriculture University, Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaShandong Lonct Enzymes Co., Ltd., Linyi, ChinaAnimal Husbandry and Fisheries Affairs Center, Huaihua, ChinaHunan Perfly Biotech Co., Ltd., Changsha, ChinaHunan Institute of Animal and Veterinary Science, Changsha, ChinaCollege of Animal Science and Technology, Hunan Agriculture University, Changsha, ChinaThe present study investigated the effects of ferulic acid (FA) on the growth performance, serum cytokine profile, intestinal morphology, and intestinal microbiota in ducks at the growing stage. 300 female Linwu ducks at 28 days of age with similar body weights were randomly divided into five groups. Each group contained six replicates of 10 birds. The dietary treatments were corn-soybean-based diet supplemented with FA at the concentrations of 0 (control), 100, 200, 400, and 800 mg/kg diet. The results demonstrated that dietary FA at the levels of 200, 400, and 800 mg/kg increased the average daily gain (P = 0.01), 400 and 800 mg/kg FA increased the final body weight (P = 0.02), 100, 200, and 800 mg/kg FA increased the serum glutathione (P = 0.01), and 100, 400, and 800 mg/kg FA increased the glutathione peroxidase activities in birds (P < 0.01). Additionally, 200, 400, and 800 mg/kg dietary FA lowered the serum levels of interleukin-2 (P = 0.02) and interleukin-6 (P = 0.04). Moreover, the morphometric study of the intestines indicated that 400 mg/kg FA decreased the crypt depth in jejunum (P = 0.01) and caecum (P = 0.04), and increased the ratio of villus height to crypt depth in jejunum (P = 0.02). Significant linear and/or quadratic relationships were found between FA concentration and the measured parameters. 16S rRNA sequencing revealed that dietary FA increased the populations of genera Faecalibacterium, Paludicola, RF39, and Faecalicoccus in the cecum (P < 0.05), whereas decreased the populations of Anaerofilum and UCG-002 (P < 0.05). The Spearman correlation analysis indicated that phylum Proteobacteria were negatively, but order Oscillospirales, and family Ruminococcaceae were positively related to the parameters of the growth performance. Phylum Bacteroidetes, class Negativicutes and family Rikenellaceae were negatively associated with the parameters of the antioxidative capability. And phylum Cyanobacteria, Elusimicrobia, and Bacteroidetes, class Bacilli, family Rikenellaceae, and genus Prevotella were positively associated with the parameters of the immunological capability. Thus, it was concluded that the supplementations of 400 mg/kg FA in diet was able to improve the growth performance, antioxidative and immunological capabilities, intestinal morphology, and modulated the gut microbial construction of Linwu ducks at the growing stage.https://www.frontiersin.org/articles/10.3389/fmicb.2021.698213/fullgut microbiotaferulic acidgrowth performanceserum cytokineintestinal morphologyduck |
| spellingShingle | Yang Liu Yang Liu Qian Lin Xuan Huang Guitao Jiang Chuang Li Xu Zhang Shengli Liu Lingyun He Yali Liu Qiuzhong Dai Xingguo Huang Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks Frontiers in Microbiology gut microbiota ferulic acid growth performance serum cytokine intestinal morphology duck |
| title | Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks |
| title_full | Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks |
| title_fullStr | Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks |
| title_full_unstemmed | Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks |
| title_short | Effects of Dietary Ferulic Acid on the Intestinal Microbiota and the Associated Changes on the Growth Performance, Serum Cytokine Profile, and Intestinal Morphology in Ducks |
| title_sort | effects of dietary ferulic acid on the intestinal microbiota and the associated changes on the growth performance serum cytokine profile and intestinal morphology in ducks |
| topic | gut microbiota ferulic acid growth performance serum cytokine intestinal morphology duck |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698213/full |
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