Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters
One of the major environmental problems is the highly toxic agro-industrial waste called olive mill wastewater (OMW), deriving from olive oil production. On the other hand, the continuous development of the biological liquid fuel industry (biodiesel and bioethanol) makes it mandatory the process and...
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MDPI AG
2019-09-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/12/19/3649 |
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| author | Markella Tzirita Maria Kremmyda Dimitris Sarris Apostolis A. Koutinas Seraphim Papanikolaou |
| author_facet | Markella Tzirita Maria Kremmyda Dimitris Sarris Apostolis A. Koutinas Seraphim Papanikolaou |
| author_sort | Markella Tzirita |
| collection | DOAJ |
| description | One of the major environmental problems is the highly toxic agro-industrial waste called olive mill wastewater (OMW), deriving from olive oil production. On the other hand, the continuous development of the biological liquid fuel industry (biodiesel and bioethanol) makes it mandatory the process and exploitation of their main by-products, crude glycerol. This study dealt with the biotechnological conversions of biodiesel-derived crude glycerol with the use of the non-conventional yeast <i>Yarrowia lipolytica</i> in media that had been diluted with OMWs. OMWs, employed as simultaneous liquid medium and substrate, is a new trend recently appearing in Industrial Biotechnology, where value-added metabolites could be produced with simultaneous partial detoxification (i.e. decolorization and phenol removal) of the used residue. In the present study, diluted OMWs (containing 2.0 g/L of total phenolic compounds) blended with 70.0 g/L crude glycerol were employed as substrates. Production of value-added compounds by <i>Y. lipolytica</i> strain ACA-YC 5031 was studied in nitrogen-limited media favoring the production of secondary metabolites (i.e. citric acid, polyols, microbial lipids, polysaccharides). Batch-flask cultures were carried out and the impact of the addition of different NaCl concentrations (1.0%, 3.0%, 5.0% <i>w</i>/<i>w</i>) added upon the biochemical behavior of the strain was studied. Remarkable biomass production was observed in all trials, while in the “blank” experiment (no OMWs and no salt added), the metabolism was shifted toward the synthesis of polyols (Σpolyols = mannitol + arabitol + erythritol > 20 g/L and maximum total citric acid-Cit (sum of citric and isocitric acid) = 10.5 g/L). Addition of OMWs resulted in <i>Cit<sub>max</sub></i> = 32.7 g/L, while Σpolyols concentration dropped to <15 g/L. Addition of salt in the OMW-based media slightly reduced the produced biomass, while <i>Cit</i> production drastically increased, reaching a final value of 54.0 g/L (conversion yield of Cit produced per unit of glycerol consumed = 0.82 g/g) in the trial with addition of 5.0% NaCl. Finally, significant color and phenols removal were observed, evaluating the yeast as a decontamination medium for the OMW and a great candidate for the production of value-added compounds. |
| first_indexed | 2024-04-11T13:02:08Z |
| format | Article |
| id | doaj.art-e353c69aaedc4e9d87dfb4f9764039dc |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
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| publishDate | 2019-09-01 |
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| spelling | doaj.art-e353c69aaedc4e9d87dfb4f9764039dc2022-12-22T04:22:55ZengMDPI AGEnergies1996-10732019-09-011219364910.3390/en12193649en12193649Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill WastewatersMarkella Tzirita0Maria Kremmyda1Dimitris Sarris2Apostolis A. Koutinas3Seraphim Papanikolaou4Department of Food Science & Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceDepartment of Food Science & Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceDepartment of Food Science & Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceDepartment of Food Science & Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceDepartment of Food Science & Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, GreeceOne of the major environmental problems is the highly toxic agro-industrial waste called olive mill wastewater (OMW), deriving from olive oil production. On the other hand, the continuous development of the biological liquid fuel industry (biodiesel and bioethanol) makes it mandatory the process and exploitation of their main by-products, crude glycerol. This study dealt with the biotechnological conversions of biodiesel-derived crude glycerol with the use of the non-conventional yeast <i>Yarrowia lipolytica</i> in media that had been diluted with OMWs. OMWs, employed as simultaneous liquid medium and substrate, is a new trend recently appearing in Industrial Biotechnology, where value-added metabolites could be produced with simultaneous partial detoxification (i.e. decolorization and phenol removal) of the used residue. In the present study, diluted OMWs (containing 2.0 g/L of total phenolic compounds) blended with 70.0 g/L crude glycerol were employed as substrates. Production of value-added compounds by <i>Y. lipolytica</i> strain ACA-YC 5031 was studied in nitrogen-limited media favoring the production of secondary metabolites (i.e. citric acid, polyols, microbial lipids, polysaccharides). Batch-flask cultures were carried out and the impact of the addition of different NaCl concentrations (1.0%, 3.0%, 5.0% <i>w</i>/<i>w</i>) added upon the biochemical behavior of the strain was studied. Remarkable biomass production was observed in all trials, while in the “blank” experiment (no OMWs and no salt added), the metabolism was shifted toward the synthesis of polyols (Σpolyols = mannitol + arabitol + erythritol > 20 g/L and maximum total citric acid-Cit (sum of citric and isocitric acid) = 10.5 g/L). Addition of OMWs resulted in <i>Cit<sub>max</sub></i> = 32.7 g/L, while Σpolyols concentration dropped to <15 g/L. Addition of salt in the OMW-based media slightly reduced the produced biomass, while <i>Cit</i> production drastically increased, reaching a final value of 54.0 g/L (conversion yield of Cit produced per unit of glycerol consumed = 0.82 g/g) in the trial with addition of 5.0% NaCl. Finally, significant color and phenols removal were observed, evaluating the yeast as a decontamination medium for the OMW and a great candidate for the production of value-added compounds.https://www.mdpi.com/1996-1073/12/19/3649Biodiesel-derived glycerolcitric acidmicrobial lipidolive-mill-wastewaterpolyolspolysaccharides<i>Yarrowia lipolytica</i>value-added compounds |
| spellingShingle | Markella Tzirita Maria Kremmyda Dimitris Sarris Apostolis A. Koutinas Seraphim Papanikolaou Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters Energies Biodiesel-derived glycerol citric acid microbial lipid olive-mill-wastewater polyols polysaccharides <i>Yarrowia lipolytica</i> value-added compounds |
| title | Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters |
| title_full | Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters |
| title_fullStr | Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters |
| title_full_unstemmed | Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters |
| title_short | Effect of Salt Addition upon the Production of Metabolic Compounds by <i>Yarrowia lipolytica</i> Cultivated on Biodiesel-Derived Glycerol Diluted with Olive-Mill Wastewaters |
| title_sort | effect of salt addition upon the production of metabolic compounds by i yarrowia lipolytica i cultivated on biodiesel derived glycerol diluted with olive mill wastewaters |
| topic | Biodiesel-derived glycerol citric acid microbial lipid olive-mill-wastewater polyols polysaccharides <i>Yarrowia lipolytica</i> value-added compounds |
| url | https://www.mdpi.com/1996-1073/12/19/3649 |
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