Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress
The high-density-dependent phase change from solitary to gregarious individuals in locusts is a typical example of phenotypic plasticity. However, the underlying molecular mechanism is not clear. In this study, first, <i>Oedaleus asiaticus</i> were treated with high-density population st...
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MDPI AG
2022-11-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/11/1034 |
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| author | Na Guo Hongyue Ma Haibin Han Feng Yan Haiyan Gao Yuanyuan Zhang Shujing Gao |
| author_facet | Na Guo Hongyue Ma Haibin Han Feng Yan Haiyan Gao Yuanyuan Zhang Shujing Gao |
| author_sort | Na Guo |
| collection | DOAJ |
| description | The high-density-dependent phase change from solitary to gregarious individuals in locusts is a typical example of phenotypic plasticity. However, the underlying molecular mechanism is not clear. In this study, first, <i>Oedaleus asiaticus</i> were treated with high-density population stress and then analyzed by Illumina sequencing on days 1, 3, 5, and 7 of the body color change to identify the stage-specific differentially expressed genes (DEGs). The KEGG pathway enrichment analysis of the identified DEGs revealed their role in metabolic pathways. Furthermore, the expression patterns of the nine key DEGs were studied in detail; this showed that the material change in locusts began on the third day of the high-density treatment, with the number of DEGs being the largest, indicating the importance of this period in the phase transition. In addition, the phenotypic change involved several key genes of important regulatory pathways, possibly working in a complex network. Phenotypic plasticity in locusts is multifactorial, involving multilevel material network interactions. This study improves the mechanistic understanding of phenotypic variation in insects at the genetic level. |
| first_indexed | 2024-03-09T18:59:07Z |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T18:59:07Z |
| publishDate | 2022-11-01 |
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| series | Insects |
| spelling | doaj.art-e35494b5fa92425db8d614ddb638c6db2023-11-24T05:13:49ZengMDPI AGInsects2075-44502022-11-011311103410.3390/insects13111034Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population StressNa Guo0Hongyue Ma1Haibin Han2Feng Yan3Haiyan Gao4Yuanyuan Zhang5Shujing Gao6Institute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010010, ChinaResearch Center for Grassland Entomology, Inner Mongolia University for Nationalities, Tongliao 028000, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010010, ChinaForest Pest Management and Quarantine Station of Ordos, Ordos 017010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010010, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot 010010, ChinaThe high-density-dependent phase change from solitary to gregarious individuals in locusts is a typical example of phenotypic plasticity. However, the underlying molecular mechanism is not clear. In this study, first, <i>Oedaleus asiaticus</i> were treated with high-density population stress and then analyzed by Illumina sequencing on days 1, 3, 5, and 7 of the body color change to identify the stage-specific differentially expressed genes (DEGs). The KEGG pathway enrichment analysis of the identified DEGs revealed their role in metabolic pathways. Furthermore, the expression patterns of the nine key DEGs were studied in detail; this showed that the material change in locusts began on the third day of the high-density treatment, with the number of DEGs being the largest, indicating the importance of this period in the phase transition. In addition, the phenotypic change involved several key genes of important regulatory pathways, possibly working in a complex network. Phenotypic plasticity in locusts is multifactorial, involving multilevel material network interactions. This study improves the mechanistic understanding of phenotypic variation in insects at the genetic level.https://www.mdpi.com/2075-4450/13/11/1034<i>Oedaleus asiaticus</i>high-density populationphenotypic plasticitytranscriptomics |
| spellingShingle | Na Guo Hongyue Ma Haibin Han Feng Yan Haiyan Gao Yuanyuan Zhang Shujing Gao Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress Insects <i>Oedaleus asiaticus</i> high-density population phenotypic plasticity transcriptomics |
| title | Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress |
| title_full | Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress |
| title_fullStr | Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress |
| title_full_unstemmed | Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress |
| title_short | Phenotypic and Differential Gene Expression Analyses of Phase Transition in <i>Oedaleus Asiaticus</i> under High-Density Population Stress |
| title_sort | phenotypic and differential gene expression analyses of phase transition in i oedaleus asiaticus i under high density population stress |
| topic | <i>Oedaleus asiaticus</i> high-density population phenotypic plasticity transcriptomics |
| url | https://www.mdpi.com/2075-4450/13/11/1034 |
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