Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus)
Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2021-08-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/37294 |
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| author | Noer Muhammad Dliyaul Haq Diah Pristihadi Vista Budiariati Dwi Budiono Al Mukhlas Fikri Mokhamad Fahrudin Cece Sumantri Arief Boediono |
| author_facet | Noer Muhammad Dliyaul Haq Diah Pristihadi Vista Budiariati Dwi Budiono Al Mukhlas Fikri Mokhamad Fahrudin Cece Sumantri Arief Boediono |
| author_sort | Noer Muhammad Dliyaul Haq |
| collection | DOAJ |
| description | Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop. |
| first_indexed | 2024-12-19T17:32:53Z |
| format | Article |
| id | doaj.art-e356c148cf3b423ebbf7a97df3d40fad |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-19T17:32:53Z |
| publishDate | 2021-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-e356c148cf3b423ebbf7a97df3d40fad2022-12-21T20:12:25ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942021-08-0128310.4308/hjb.28.3.185Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus)Noer Muhammad Dliyaul Haq0Diah Pristihadi1Vista Budiariati2Dwi Budiono3Al Mukhlas Fikri4Mokhamad Fahrudin5Cece Sumantri6Arief Boediono71Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor,Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartement of Community Nutrition, Faculty of Human Ecology, IPB University, BogorDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartment of Animal Production and Technology, Faculty of Animal Science, IPB University, BogorDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorInterspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.https://journal.ipb.ac.id/index.php/hayati/article/view/37294 |
| spellingShingle | Noer Muhammad Dliyaul Haq Diah Pristihadi Vista Budiariati Dwi Budiono Al Mukhlas Fikri Mokhamad Fahrudin Cece Sumantri Arief Boediono Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Hayati Journal of Biosciences |
| title | Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) |
| title_full | Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) |
| title_fullStr | Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) |
| title_full_unstemmed | Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) |
| title_short | Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) |
| title_sort | interspecies reconstructed embryonic cell interaction between campbell hamster phodopus campbelli and mice mus musculus |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/37294 |
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