Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece
Due to botanical tulips’ economic interest coupled with limited information regarding their seed germination, we investigated the effect of temperature on dormancy release and germination in two endangered local endemic tulip species of Greece (<i>Tulipa hageri</i> Heldr., <i>T. or...
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/9/1859 |
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| author | Elias Pipinis Stefanos Hatzilazarou Stefanos Kostas Rafaela Stagiopoulou Konstantina Gitsa Eleftherios Dariotis Ioulietta Samartza Ioannis Plastiras Eleni Kriemadi Pepy Bareka Christos Lykas Georgios Tsoktouridis Nikos Krigas |
| author_facet | Elias Pipinis Stefanos Hatzilazarou Stefanos Kostas Rafaela Stagiopoulou Konstantina Gitsa Eleftherios Dariotis Ioulietta Samartza Ioannis Plastiras Eleni Kriemadi Pepy Bareka Christos Lykas Georgios Tsoktouridis Nikos Krigas |
| author_sort | Elias Pipinis |
| collection | DOAJ |
| description | Due to botanical tulips’ economic interest coupled with limited information regarding their seed germination, we investigated the effect of temperature on dormancy release and germination in two endangered local endemic tulip species of Greece (<i>Tulipa hageri</i> Heldr., <i>T. orphanidea</i> Heldr.). Their germination responses at five constant temperatures (5, 10, 15, 20, and 25 °C) were evaluated in growth chambers, while the type of seed dormancy and the temperature effect on its release were determined based on open-sourced, R-derived species-specific ecological profiles illustrating abiotic conditions of their wild habitats. The results indicated a range-specific temperature dependence in seed germination for both studied species with seed germination observed only in very low temperatures (5–10 °C). The seeds of both species after dispersal had an underdeveloped embryo. The existence of a complex morphophysiological seed dormancy was confirmed in both species by the significant embryo development only at 5 and 10 °C (almost doubled after 30 days) coupled with observed delay in germination only at low temperatures. Furthermore, to facilitate their cultivation and ex situ conservation, the germinated seeds were planted in pots to develop bulblets in greenhouse conditions resulting in bigger <i>T. orphanidea</i> bulblets compared to <i>T. hageri</i>. |
| first_indexed | 2024-03-11T04:10:34Z |
| format | Article |
| id | doaj.art-e3575c5b794e4b0b8ef4d48a1fd5486a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T04:10:34Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-e3575c5b794e4b0b8ef4d48a1fd5486a2023-11-17T23:32:42ZengMDPI AGPlants2223-77472023-04-01129185910.3390/plants12091859Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from GreeceElias Pipinis0Stefanos Hatzilazarou1Stefanos Kostas2Rafaela Stagiopoulou3Konstantina Gitsa4Eleftherios Dariotis5Ioulietta Samartza6Ioannis Plastiras7Eleni Kriemadi8Pepy Bareka9Christos Lykas10Georgios Tsoktouridis11Nikos Krigas12Laboratory of Silviculture, School of Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceLaboratory of Floriculture, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceThermokipia Athina, 57500 Epanomi, GreeceLaboratory of Systematic Botany, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceLaboratory of Systematic Botany, Department of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, GreeceDepartment of Agriculture, Crop Production and Rural Environment, School of Agricultural Sciences, University of Thessaly, 38446 Volos, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceInstitute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thessaloniki, GreeceDue to botanical tulips’ economic interest coupled with limited information regarding their seed germination, we investigated the effect of temperature on dormancy release and germination in two endangered local endemic tulip species of Greece (<i>Tulipa hageri</i> Heldr., <i>T. orphanidea</i> Heldr.). Their germination responses at five constant temperatures (5, 10, 15, 20, and 25 °C) were evaluated in growth chambers, while the type of seed dormancy and the temperature effect on its release were determined based on open-sourced, R-derived species-specific ecological profiles illustrating abiotic conditions of their wild habitats. The results indicated a range-specific temperature dependence in seed germination for both studied species with seed germination observed only in very low temperatures (5–10 °C). The seeds of both species after dispersal had an underdeveloped embryo. The existence of a complex morphophysiological seed dormancy was confirmed in both species by the significant embryo development only at 5 and 10 °C (almost doubled after 30 days) coupled with observed delay in germination only at low temperatures. Furthermore, to facilitate their cultivation and ex situ conservation, the germinated seeds were planted in pots to develop bulblets in greenhouse conditions resulting in bigger <i>T. orphanidea</i> bulblets compared to <i>T. hageri</i>.https://www.mdpi.com/2223-7747/12/9/1859botanical tulipsbulbous plantsecological requirementsRMediterranean biodiversityR-derived ecological profilingthreatened species |
| spellingShingle | Elias Pipinis Stefanos Hatzilazarou Stefanos Kostas Rafaela Stagiopoulou Konstantina Gitsa Eleftherios Dariotis Ioulietta Samartza Ioannis Plastiras Eleni Kriemadi Pepy Bareka Christos Lykas Georgios Tsoktouridis Nikos Krigas Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece Plants botanical tulips bulbous plants ecological requirements RMediterranean biodiversity R-derived ecological profiling threatened species |
| title | Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece |
| title_full | Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece |
| title_fullStr | Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece |
| title_full_unstemmed | Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece |
| title_short | Effect of Temperature on Breaking of Morphophysiological Dormancy and Seed Germination Leading to Bulblet Production in Two Endemic Tulip Species from Greece |
| title_sort | effect of temperature on breaking of morphophysiological dormancy and seed germination leading to bulblet production in two endemic tulip species from greece |
| topic | botanical tulips bulbous plants ecological requirements RMediterranean biodiversity R-derived ecological profiling threatened species |
| url | https://www.mdpi.com/2223-7747/12/9/1859 |
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