Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis
<i>Panax ginseng</i> is widely used in Asian countries and its active constituents—ginsenosides—need to be systematically studied. However, only a small part of ginsenosides have been characterized in the roots and rhizomes of <i>panax ginseng</i> (RRPG) up to date, mainly be...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/941 |
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| author | Hong-Ping Wang Zi-Jian Wang Jing Du Zhao-Zhou Lin Chen Zhao Run Zhang Qiong Yin Chun-Lan Fan Ping Peng Zhi-Bin Wang |
| author_facet | Hong-Ping Wang Zi-Jian Wang Jing Du Zhao-Zhou Lin Chen Zhao Run Zhang Qiong Yin Chun-Lan Fan Ping Peng Zhi-Bin Wang |
| author_sort | Hong-Ping Wang |
| collection | DOAJ |
| description | <i>Panax ginseng</i> is widely used in Asian countries and its active constituents—ginsenosides—need to be systematically studied. However, only a small part of ginsenosides have been characterized in the roots and rhizomes of <i>panax ginseng</i> (RRPG) up to date, mainly because of a lack of the fragmentation ions of many more ginsenosides. In order to comprehensively identify ginsenosides in RRPG, molecular features of ginsenosides orienting precursor ions selection and targeted tandem mass spectrometry (MS/MS) analysis strategy were proposed in our study, in which the precursor ions were selected according to the molecular features of ginsenosides irrespective of their peak abundances, and targeted MS/MS analysis was then performed to obtain their fragmentation ions for substance characterization. Using this strategy, a total of 620 ginsenosides were successfully characterized in RRPG, including 309 protopanaxadiol-type ginsenosides, 258 protopanaxatriol-type ginsenosides and 53 oleanane-type ginsenosides. It is worth noting that, except for the known aglycones mass-to-charge ratio (<i>m/z</i>) 459, 475 and 455, twelve other aglycones, including <i>m/z</i> 509, 507, 493, 491, 489, 487, 477, 473, 461, 457, 443 and 441, were first reported in our experiment and they were probably the derivatizations of the protopanaxatriol and protopanaxadiol. Our study will not only help people to improve the cognition of ginsenosides in RRPG, but will also play a guiding and reference role for the isolation and characterization of potentially new ginsenosides from RRPG. |
| first_indexed | 2024-03-11T09:34:49Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:34:49Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e358218262b5493eadc6790881a7067f2023-11-16T17:25:22ZengMDPI AGMolecules1420-30492023-01-0128394110.3390/molecules28030941Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS AnalysisHong-Ping Wang0Zi-Jian Wang1Jing Du2Zhao-Zhou Lin3Chen Zhao4Run Zhang5Qiong Yin6Chun-Lan Fan7Ping Peng8Zhi-Bin Wang9Scientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaBeijing Tongrentang Technology Development Co., Ltd., Beijing 100079, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaBeijing Zhongyan Tongrentang Pharmaceutical R & D Co., Ltd., Beijing 100000, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaBeijing Tongrentang Technology Development Co., Ltd., Beijing 100079, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, ChinaScientific Research Institute of Beijing Tongrentang Co., Ltd., Beijing 100011, China<i>Panax ginseng</i> is widely used in Asian countries and its active constituents—ginsenosides—need to be systematically studied. However, only a small part of ginsenosides have been characterized in the roots and rhizomes of <i>panax ginseng</i> (RRPG) up to date, mainly because of a lack of the fragmentation ions of many more ginsenosides. In order to comprehensively identify ginsenosides in RRPG, molecular features of ginsenosides orienting precursor ions selection and targeted tandem mass spectrometry (MS/MS) analysis strategy were proposed in our study, in which the precursor ions were selected according to the molecular features of ginsenosides irrespective of their peak abundances, and targeted MS/MS analysis was then performed to obtain their fragmentation ions for substance characterization. Using this strategy, a total of 620 ginsenosides were successfully characterized in RRPG, including 309 protopanaxadiol-type ginsenosides, 258 protopanaxatriol-type ginsenosides and 53 oleanane-type ginsenosides. It is worth noting that, except for the known aglycones mass-to-charge ratio (<i>m/z</i>) 459, 475 and 455, twelve other aglycones, including <i>m/z</i> 509, 507, 493, 491, 489, 487, 477, 473, 461, 457, 443 and 441, were first reported in our experiment and they were probably the derivatizations of the protopanaxatriol and protopanaxadiol. Our study will not only help people to improve the cognition of ginsenosides in RRPG, but will also play a guiding and reference role for the isolation and characterization of potentially new ginsenosides from RRPG.https://www.mdpi.com/1420-3049/28/3/941ginsenosidesLC-MS<i>panax ginseng</i>qualitative analysisnew strategy |
| spellingShingle | Hong-Ping Wang Zi-Jian Wang Jing Du Zhao-Zhou Lin Chen Zhao Run Zhang Qiong Yin Chun-Lan Fan Ping Peng Zhi-Bin Wang Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis Molecules ginsenosides LC-MS <i>panax ginseng</i> qualitative analysis new strategy |
| title | Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis |
| title_full | Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis |
| title_fullStr | Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis |
| title_full_unstemmed | Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis |
| title_short | Comprehensive Identification of Ginsenosides in the Roots and Rhizomes of <i>Panax ginseng</i> Based on Their Molecular Features-Oriented Precursor Ions Selection and Targeted MS/MS Analysis |
| title_sort | comprehensive identification of ginsenosides in the roots and rhizomes of i panax ginseng i based on their molecular features oriented precursor ions selection and targeted ms ms analysis |
| topic | ginsenosides LC-MS <i>panax ginseng</i> qualitative analysis new strategy |
| url | https://www.mdpi.com/1420-3049/28/3/941 |
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