Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis
Lang Wang,1,2,* Zhiqi Zhang,1,3,* Xingxiang Chen,1 Fang Yu,4 Hai Huang,4 Xue Shen,4 Yujie Tan,4 Qingqing Wu4 1Department of Microbiology and Immunology, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, People’s Republic of China; 2Hunan University of Medici...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-04-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/relationship-between-the-expression-of-pd-1-and-ctla-4-on-t-lymphocyte-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1797839223910301696 |
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| author | Wang L Zhang Z Chen X Yu F Huang H Shen X Tan Y Wu Q |
| author_facet | Wang L Zhang Z Chen X Yu F Huang H Shen X Tan Y Wu Q |
| author_sort | Wang L |
| collection | DOAJ |
| description | Lang Wang,1,2,* Zhiqi Zhang,1,3,* Xingxiang Chen,1 Fang Yu,4 Hai Huang,4 Xue Shen,4 Yujie Tan,4 Qingqing Wu4 1Department of Microbiology and Immunology, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, People’s Republic of China; 2Hunan University of Medicine, Huaihua, People’s Republic of China; 3Taian City Central Hospital Affiliated to Qingdao University, Taian, People’s Republic of China; 4Center of Clinical Laboratory, the Affiliated Hospital of Guizhou Medical University, Guiyang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qingqing Wu, Center of Clinical Laboratory, the Affiliated Hospital of Guizhou Medical University, Guiyang, People’s Republic of China, Email wuqingqing@gmc.edu.cnPurpose: The study aimed to investigate the relationship between the expression of PD-1 and CTLA-4 on the surface of peripheral blood T lymphocyte subsets in patients with sepsis and the severity and prognosis of the disease.Patients and Methods: The study included patients with sepsis who were admitted to the intensive care unit. The expression of PD-1 and CTLA-4 on T lymphocyte subsets was detected by flow cytometry, and the severity of sepsis was assessed using the SOFA score.Results: The expression of PD-1 on CD4+T cells, PD-1 on Tregs, and CTLA-4 on Tregs increased with the severity of the disease (P< 0.05). Multivariate logistic regression analysis showed that PD-1 expression on CD4+T cells, CTLA-4 expression on Tregs, and the SOFA score were independent risk factors for 28-day mortality in patients with sepsis (P< 0.05). The area under the curve of the SOFA score combined with the expression of PD-1 on CD4+T cells and CTLA-4 on Treg cells was significantly higher than any single indicator (P< 0.05). Patients with high expression of PD-1 on CD4+T cells (> 31.25%) and CTLA-4 on Tregs (> 12.64%) had a lower 28-day survival rate (P< 0.05).Conclusion: The increased expression of PD-1 and CTLA-4 on CD4+T cells and Tregs is significantly associated with the severity and prognosis of sepsis patients. The combination of the SOFA score and the expression of PD-1 on CD4+T cells and CTLA-4 on Tregs can further improve the prognostic predictive value. These findings may be promising biomarkers for prognostic assessment, risk stratification, and identification of immunosuppression in patients with sepsis.Keywords: sepsis, T lymphocyte subsets, programmed cell death receptor-1, cytotoxic T lymphocyte antigen-4, prognosis |
| first_indexed | 2024-04-09T15:54:37Z |
| format | Article |
| id | doaj.art-e35abacb637f42838287b628cfb892d3 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-04-09T15:54:37Z |
| publishDate | 2023-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-e35abacb637f42838287b628cfb892d32023-04-25T18:55:18ZengDove Medical PressInternational Journal of General Medicine1178-70742023-04-01Volume 161513152583286Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of SepsisWang LZhang ZChen XYu FHuang HShen XTan YWu QLang Wang,1,2,* Zhiqi Zhang,1,3,* Xingxiang Chen,1 Fang Yu,4 Hai Huang,4 Xue Shen,4 Yujie Tan,4 Qingqing Wu4 1Department of Microbiology and Immunology, School of Clinical Laboratory Science, Guizhou Medical University, Guiyang, People’s Republic of China; 2Hunan University of Medicine, Huaihua, People’s Republic of China; 3Taian City Central Hospital Affiliated to Qingdao University, Taian, People’s Republic of China; 4Center of Clinical Laboratory, the Affiliated Hospital of Guizhou Medical University, Guiyang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qingqing Wu, Center of Clinical Laboratory, the Affiliated Hospital of Guizhou Medical University, Guiyang, People’s Republic of China, Email wuqingqing@gmc.edu.cnPurpose: The study aimed to investigate the relationship between the expression of PD-1 and CTLA-4 on the surface of peripheral blood T lymphocyte subsets in patients with sepsis and the severity and prognosis of the disease.Patients and Methods: The study included patients with sepsis who were admitted to the intensive care unit. The expression of PD-1 and CTLA-4 on T lymphocyte subsets was detected by flow cytometry, and the severity of sepsis was assessed using the SOFA score.Results: The expression of PD-1 on CD4+T cells, PD-1 on Tregs, and CTLA-4 on Tregs increased with the severity of the disease (P< 0.05). Multivariate logistic regression analysis showed that PD-1 expression on CD4+T cells, CTLA-4 expression on Tregs, and the SOFA score were independent risk factors for 28-day mortality in patients with sepsis (P< 0.05). The area under the curve of the SOFA score combined with the expression of PD-1 on CD4+T cells and CTLA-4 on Treg cells was significantly higher than any single indicator (P< 0.05). Patients with high expression of PD-1 on CD4+T cells (> 31.25%) and CTLA-4 on Tregs (> 12.64%) had a lower 28-day survival rate (P< 0.05).Conclusion: The increased expression of PD-1 and CTLA-4 on CD4+T cells and Tregs is significantly associated with the severity and prognosis of sepsis patients. The combination of the SOFA score and the expression of PD-1 on CD4+T cells and CTLA-4 on Tregs can further improve the prognostic predictive value. These findings may be promising biomarkers for prognostic assessment, risk stratification, and identification of immunosuppression in patients with sepsis.Keywords: sepsis, T lymphocyte subsets, programmed cell death receptor-1, cytotoxic T lymphocyte antigen-4, prognosishttps://www.dovepress.com/relationship-between-the-expression-of-pd-1-and-ctla-4-on-t-lymphocyte-peer-reviewed-fulltext-article-IJGMsepsist lymphocyte subsetsprogrammed cell death receptor-1cytotoxic t lymphocyte antigen-4prognosis |
| spellingShingle | Wang L Zhang Z Chen X Yu F Huang H Shen X Tan Y Wu Q Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis International Journal of General Medicine sepsis t lymphocyte subsets programmed cell death receptor-1 cytotoxic t lymphocyte antigen-4 prognosis |
| title | Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis |
| title_full | Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis |
| title_fullStr | Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis |
| title_full_unstemmed | Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis |
| title_short | Relationship Between the Expression of PD-1 and CTLA-4 on T Lymphocytes and the Severity and Prognosis of Sepsis |
| title_sort | relationship between the expression of pd 1 and ctla 4 on t lymphocytes and the severity and prognosis of sepsis |
| topic | sepsis t lymphocyte subsets programmed cell death receptor-1 cytotoxic t lymphocyte antigen-4 prognosis |
| url | https://www.dovepress.com/relationship-between-the-expression-of-pd-1-and-ctla-4-on-t-lymphocyte-peer-reviewed-fulltext-article-IJGM |
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