Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells
Numerous documented ethnopharmacological properties have been associated with Swietenia macrophylla (Meliaceae), with its seed extract reported to display anti-hypoglycemic activities in diabetic rats. In the present study, three compounds isolated from the seeds of S. macrophylla were tested on a m...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/20/12/19847 |
| _version_ | 1828974889411805184 |
|---|---|
| author | Wai Kwan Lau Bey Hing Goh Habsah Abdul Kadir Alexander Chong Shu-Chien Tengku Sifzizul Tengku Muhammad |
| author_facet | Wai Kwan Lau Bey Hing Goh Habsah Abdul Kadir Alexander Chong Shu-Chien Tengku Sifzizul Tengku Muhammad |
| author_sort | Wai Kwan Lau |
| collection | DOAJ |
| description | Numerous documented ethnopharmacological properties have been associated with Swietenia macrophylla (Meliaceae), with its seed extract reported to display anti-hypoglycemic activities in diabetic rats. In the present study, three compounds isolated from the seeds of S. macrophylla were tested on a modified ELISA binding assay and showed to possess PPARγ ligand activity. They were corresponded to PPARγ-mediated cellular response, stimulated adipocyte differentiation but produced lower amount of fat droplets compared to a conventional anti-diabetic agent, rosiglitazone. The up-regulation of adipocytes was followed by increased adipocyte-related gene expressions such as adiponectin, adipsin, and PPARγ. The S. macrophylla compounds also promoted cellular glucose uptake via the translocation of GLUT4 glucose transporter. |
| first_indexed | 2024-12-14T14:10:27Z |
| format | Article |
| id | doaj.art-e35d7dc7c3464c9385004eacbcfc9370 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-14T14:10:27Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e35d7dc7c3464c9385004eacbcfc93702022-12-21T22:58:19ZengMDPI AGMolecules1420-30492015-12-012012223012231410.3390/molecules201219847molecules201219847Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle CellsWai Kwan Lau0Bey Hing Goh1Habsah Abdul Kadir2Alexander Chong Shu-Chien3Tengku Sifzizul Tengku Muhammad4Experimental Therapeutics Center, Institute of Pharmaceuticals and Nutraceuticals Malaysia, Blok 5-A, Halaman Bukit Gambir, Penang 11700, MalaysiaBiomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaBiomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, MalaysiaExperimental Therapeutics Center, Institute of Pharmaceuticals and Nutraceuticals Malaysia, Blok 5-A, Halaman Bukit Gambir, Penang 11700, MalaysiaExperimental Therapeutics Center, Institute of Pharmaceuticals and Nutraceuticals Malaysia, Blok 5-A, Halaman Bukit Gambir, Penang 11700, MalaysiaNumerous documented ethnopharmacological properties have been associated with Swietenia macrophylla (Meliaceae), with its seed extract reported to display anti-hypoglycemic activities in diabetic rats. In the present study, three compounds isolated from the seeds of S. macrophylla were tested on a modified ELISA binding assay and showed to possess PPARγ ligand activity. They were corresponded to PPARγ-mediated cellular response, stimulated adipocyte differentiation but produced lower amount of fat droplets compared to a conventional anti-diabetic agent, rosiglitazone. The up-regulation of adipocytes was followed by increased adipocyte-related gene expressions such as adiponectin, adipsin, and PPARγ. The S. macrophylla compounds also promoted cellular glucose uptake via the translocation of GLUT4 glucose transporter.http://www.mdpi.com/1420-3049/20/12/19847Swietenia macrophyllaPPARγ ligandGLUT4 translocationglucose uptake |
| spellingShingle | Wai Kwan Lau Bey Hing Goh Habsah Abdul Kadir Alexander Chong Shu-Chien Tengku Sifzizul Tengku Muhammad Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells Molecules Swietenia macrophylla PPARγ ligand GLUT4 translocation glucose uptake |
| title | Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells |
| title_full | Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells |
| title_fullStr | Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells |
| title_full_unstemmed | Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells |
| title_short | Potent PPARγ Ligands from Swietenia macrophylla Are Capable of Stimulating Glucose Uptake in Muscle Cells |
| title_sort | potent pparγ ligands from swietenia macrophylla are capable of stimulating glucose uptake in muscle cells |
| topic | Swietenia macrophylla PPARγ ligand GLUT4 translocation glucose uptake |
| url | http://www.mdpi.com/1420-3049/20/12/19847 |
| work_keys_str_mv | AT waikwanlau potentppargligandsfromswieteniamacrophyllaarecapableofstimulatingglucoseuptakeinmusclecells AT beyhinggoh potentppargligandsfromswieteniamacrophyllaarecapableofstimulatingglucoseuptakeinmusclecells AT habsahabdulkadir potentppargligandsfromswieteniamacrophyllaarecapableofstimulatingglucoseuptakeinmusclecells AT alexanderchongshuchien potentppargligandsfromswieteniamacrophyllaarecapableofstimulatingglucoseuptakeinmusclecells AT tengkusifzizultengkumuhammad potentppargligandsfromswieteniamacrophyllaarecapableofstimulatingglucoseuptakeinmusclecells |