Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease
<p>Abstract</p> <p>Introduction</p> <p>Inflammation contributes to cardiovascular disease and is exacerbated with increased adiposity, particularly omental adiposity; however, the role of epicardial fat is poorly understood.</p> <p>Methods</p> <p>...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-01-01
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| Series: | Cardiovascular Diabetology |
| Online Access: | http://www.cardiab.com/content/5/1/1 |
| _version_ | 1818783139924803584 |
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| author | Kumar Sudhesh Bonser Robert S Pagano Domenico Harte Alison L Quinn David W da Silva Nancy F Baker Adam R McTernan Philip G |
| author_facet | Kumar Sudhesh Bonser Robert S Pagano Domenico Harte Alison L Quinn David W da Silva Nancy F Baker Adam R McTernan Philip G |
| author_sort | Kumar Sudhesh |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Introduction</p> <p>Inflammation contributes to cardiovascular disease and is exacerbated with increased adiposity, particularly omental adiposity; however, the role of epicardial fat is poorly understood.</p> <p>Methods</p> <p>For these studies the expression of inflammatory markers was assessed in epicardial fat biopsies from coronary artery bypass grafting (CABG) patients using quantitative RT-PCR. Further, the effects of chronic medications, including statins, as well as peri-operative glucose, insulin and potassium infusion, on gene expression were also assessed. Circulating resistin, CRP, adiponectin and leptin levels were determined to assess inflammation.</p> <p>Results</p> <p>The expression of adiponectin, resistin and other adipocytokine mRNAs were comparable to that in omental fat. Epicardial CD45 expression was significantly higher than control depots (p < 0.01) indicating significant infiltration of macrophages. Statin treated patients showed significantly lower epicardial expression of IL-6 mRNA, in comparison with the control abdominal depots (p < 0.001). The serum profile of CABG patients showed significantly higher levels of both CRP (control: 1.28 ± 1.57 μg/mL vs CABG: 9.11 ± 15.7 μg/mL; p < 0.001) and resistin (control: 10.53 ± 0.81 ng/mL vs CABG: 16.8 ± 1.69 ng/mL; p < 0.01) and significantly lower levels of adiponectin (control: 29.1 ± 14.8 μg/mL vs CABG: 11.9 ± 6.0 μg/mL; p < 0.05) when compared to BMI matched controls.</p> <p>Conclusion</p> <p>Epicardial and omental fat exhibit a broadly comparable pathogenic mRNA profile, this may arise in part from macrophage infiltration into the epicardial fat. This study highlights that chronic inflammation occurs locally as well as systemically potentially contributing further to the pathogenesis of coronary artery disease.</p> |
| first_indexed | 2024-12-18T12:59:27Z |
| format | Article |
| id | doaj.art-e35f36855ac84ec98117eea6264edf0e |
| institution | Directory Open Access Journal |
| issn | 1475-2840 |
| language | English |
| last_indexed | 2024-12-18T12:59:27Z |
| publishDate | 2006-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cardiovascular Diabetology |
| spelling | doaj.art-e35f36855ac84ec98117eea6264edf0e2022-12-21T21:07:12ZengBMCCardiovascular Diabetology1475-28402006-01-0151110.1186/1475-2840-5-1Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular diseaseKumar SudheshBonser Robert SPagano DomenicoHarte Alison LQuinn David Wda Silva Nancy FBaker Adam RMcTernan Philip G<p>Abstract</p> <p>Introduction</p> <p>Inflammation contributes to cardiovascular disease and is exacerbated with increased adiposity, particularly omental adiposity; however, the role of epicardial fat is poorly understood.</p> <p>Methods</p> <p>For these studies the expression of inflammatory markers was assessed in epicardial fat biopsies from coronary artery bypass grafting (CABG) patients using quantitative RT-PCR. Further, the effects of chronic medications, including statins, as well as peri-operative glucose, insulin and potassium infusion, on gene expression were also assessed. Circulating resistin, CRP, adiponectin and leptin levels were determined to assess inflammation.</p> <p>Results</p> <p>The expression of adiponectin, resistin and other adipocytokine mRNAs were comparable to that in omental fat. Epicardial CD45 expression was significantly higher than control depots (p < 0.01) indicating significant infiltration of macrophages. Statin treated patients showed significantly lower epicardial expression of IL-6 mRNA, in comparison with the control abdominal depots (p < 0.001). The serum profile of CABG patients showed significantly higher levels of both CRP (control: 1.28 ± 1.57 μg/mL vs CABG: 9.11 ± 15.7 μg/mL; p < 0.001) and resistin (control: 10.53 ± 0.81 ng/mL vs CABG: 16.8 ± 1.69 ng/mL; p < 0.01) and significantly lower levels of adiponectin (control: 29.1 ± 14.8 μg/mL vs CABG: 11.9 ± 6.0 μg/mL; p < 0.05) when compared to BMI matched controls.</p> <p>Conclusion</p> <p>Epicardial and omental fat exhibit a broadly comparable pathogenic mRNA profile, this may arise in part from macrophage infiltration into the epicardial fat. This study highlights that chronic inflammation occurs locally as well as systemically potentially contributing further to the pathogenesis of coronary artery disease.</p>http://www.cardiab.com/content/5/1/1 |
| spellingShingle | Kumar Sudhesh Bonser Robert S Pagano Domenico Harte Alison L Quinn David W da Silva Nancy F Baker Adam R McTernan Philip G Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease Cardiovascular Diabetology |
| title | Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| title_full | Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| title_fullStr | Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| title_full_unstemmed | Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| title_short | Human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| title_sort | human epicardial adipose tissue expresses a pathogenic profile of adipocytokines in patients with cardiovascular disease |
| url | http://www.cardiab.com/content/5/1/1 |
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