A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand
Abstract Background The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An....
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3494-8 |
| _version_ | 1818543980622643200 |
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| author | Laura Brosseau Chanya Udom Chutipong Sukkanon Theeraphap Chareonviriyaphap Michael J. Bangs Atiporn Saeung Sylvie Manguin |
| author_facet | Laura Brosseau Chanya Udom Chutipong Sukkanon Theeraphap Chareonviriyaphap Michael J. Bangs Atiporn Saeung Sylvie Manguin |
| author_sort | Laura Brosseau |
| collection | DOAJ |
| description | Abstract Background The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. Results For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. Conclusions This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand. |
| first_indexed | 2024-12-11T22:42:30Z |
| format | Article |
| id | doaj.art-e35f7e8d423449e289d9be7ad8996952 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-11T22:42:30Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-e35f7e8d423449e289d9be7ad89969522022-12-22T00:47:45ZengBMCParasites & Vectors1756-33052019-05-0112111010.1186/s13071-019-3494-8A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in ThailandLaura Brosseau0Chanya Udom1Chutipong Sukkanon2Theeraphap Chareonviriyaphap3Michael J. Bangs4Atiporn Saeung5Sylvie Manguin6HydroSciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD), CNRS, Université MontpellierDepartment of Zoology, Faculty of Science, Kasetsart UniversityDepartment of Entomology, Faculty of Agriculture, Kasetsart UniversityDepartment of Entomology, Faculty of Agriculture, Kasetsart UniversityDepartment of Entomology, Faculty of Agriculture, Kasetsart UniversityDepartment of Parasitology, Faculty of Medicine, Chiang Mai UniversityHydroSciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD), CNRS, Université MontpellierAbstract Background The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents. Results For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens. Conclusions This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.http://link.springer.com/article/10.1186/s13071-019-3494-8AnophelesBarbirostris ComplexITS2multiplex PCRThailand |
| spellingShingle | Laura Brosseau Chanya Udom Chutipong Sukkanon Theeraphap Chareonviriyaphap Michael J. Bangs Atiporn Saeung Sylvie Manguin A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand Parasites & Vectors Anopheles Barbirostris Complex ITS2 multiplex PCR Thailand |
| title | A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand |
| title_full | A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand |
| title_fullStr | A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand |
| title_full_unstemmed | A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand |
| title_short | A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand |
| title_sort | multiplex pcr assay for the identification of five species of the anopheles barbirostris complex in thailand |
| topic | Anopheles Barbirostris Complex ITS2 multiplex PCR Thailand |
| url | http://link.springer.com/article/10.1186/s13071-019-3494-8 |
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