An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections.
Though many methods can be used to identify cell types contained in complex tissues, most require cell disaggregation and destroy information about where cells reside in relation to their microenvironment. Here, we describe a polytomous key for cell type identification in intact sections of adult mo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5690684?pdf=render |
| _version_ | 1818300544714801152 |
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| author | Kyle A Wegner Mark T Cadena Ryan Trevena Anne E Turco Adam Gottschalk Richard B Halberg Jinjin Guo Jill A McMahon Andrew P McMahon Chad M Vezina |
| author_facet | Kyle A Wegner Mark T Cadena Ryan Trevena Anne E Turco Adam Gottschalk Richard B Halberg Jinjin Guo Jill A McMahon Andrew P McMahon Chad M Vezina |
| author_sort | Kyle A Wegner |
| collection | DOAJ |
| description | Though many methods can be used to identify cell types contained in complex tissues, most require cell disaggregation and destroy information about where cells reside in relation to their microenvironment. Here, we describe a polytomous key for cell type identification in intact sections of adult mouse prostate and prostatic urethra. The key is organized as a decision tree and initiates with one round of immunostaining for nerve, epithelial, fibromuscular/hematolymphoid, or vascular associated cells. Cell identities are recursively eliminated by subsequent staining events until the remaining pool of potential cell types can be distinguished by direct comparison to other cells. We validated our identification key using wild type adult mouse prostate and urethra tissue sections and it currently resolves sixteen distinct cell populations which include three nerve fiber types as well as four epithelial, five fibromuscular/hematolymphoid, one nerve-associated, and three vascular-associated cell types. We demonstrate two uses of this novel identification methodology. We first used the identification key to characterize prostate stromal cell type changes in response to constitutive phosphatidylinositide-3-kinase activation in prostate epithelium. We then used the key to map cell lineages in a new reporter mouse strain driven by Wnt10aem1(cre/ERT2)Amc. The identification key facilitates rigorous and reproducible cell identification in prostate tissue sections and can be expanded to resolve additional cell types as new antibodies and other resources become available. |
| first_indexed | 2024-12-13T05:08:48Z |
| format | Article |
| id | doaj.art-e35fe9218e0443399e0da50c09c4763e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T05:08:48Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e35fe9218e0443399e0da50c09c4763e2022-12-21T23:58:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018841310.1371/journal.pone.0188413An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections.Kyle A WegnerMark T CadenaRyan TrevenaAnne E TurcoAdam GottschalkRichard B HalbergJinjin GuoJill A McMahonAndrew P McMahonChad M VezinaThough many methods can be used to identify cell types contained in complex tissues, most require cell disaggregation and destroy information about where cells reside in relation to their microenvironment. Here, we describe a polytomous key for cell type identification in intact sections of adult mouse prostate and prostatic urethra. The key is organized as a decision tree and initiates with one round of immunostaining for nerve, epithelial, fibromuscular/hematolymphoid, or vascular associated cells. Cell identities are recursively eliminated by subsequent staining events until the remaining pool of potential cell types can be distinguished by direct comparison to other cells. We validated our identification key using wild type adult mouse prostate and urethra tissue sections and it currently resolves sixteen distinct cell populations which include three nerve fiber types as well as four epithelial, five fibromuscular/hematolymphoid, one nerve-associated, and three vascular-associated cell types. We demonstrate two uses of this novel identification methodology. We first used the identification key to characterize prostate stromal cell type changes in response to constitutive phosphatidylinositide-3-kinase activation in prostate epithelium. We then used the key to map cell lineages in a new reporter mouse strain driven by Wnt10aem1(cre/ERT2)Amc. The identification key facilitates rigorous and reproducible cell identification in prostate tissue sections and can be expanded to resolve additional cell types as new antibodies and other resources become available.http://europepmc.org/articles/PMC5690684?pdf=render |
| spellingShingle | Kyle A Wegner Mark T Cadena Ryan Trevena Anne E Turco Adam Gottschalk Richard B Halberg Jinjin Guo Jill A McMahon Andrew P McMahon Chad M Vezina An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. PLoS ONE |
| title | An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. |
| title_full | An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. |
| title_fullStr | An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. |
| title_full_unstemmed | An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. |
| title_short | An immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections. |
| title_sort | immunohistochemical identification key for cell types in adult mouse prostatic and urethral tissue sections |
| url | http://europepmc.org/articles/PMC5690684?pdf=render |
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