Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019
IntroductionPaphiopedilum barbigerum is currently the rarest and most endangered species of orchids in China and has significant ornamental value. The mature seeds of P. barbigerum are difficult to germinate owing to the absence of an endosperm and are highly dependent on mycorrhizal fungi for germi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358137/full |
| _version_ | 1797258624088670208 |
|---|---|
| author | Fan Tian Fan Tian Juncai Wang Fangjun Ding Fangjun Ding Lianhui Wang Lianhui Wang Yanbing Yang Yanbing Yang Xinxiang Bai Chengjiang Tan Xiaofeng Liao |
| author_facet | Fan Tian Fan Tian Juncai Wang Fangjun Ding Fangjun Ding Lianhui Wang Lianhui Wang Yanbing Yang Yanbing Yang Xinxiang Bai Chengjiang Tan Xiaofeng Liao |
| author_sort | Fan Tian |
| collection | DOAJ |
| description | IntroductionPaphiopedilum barbigerum is currently the rarest and most endangered species of orchids in China and has significant ornamental value. The mature seeds of P. barbigerum are difficult to germinate owing to the absence of an endosperm and are highly dependent on mycorrhizal fungi for germination and subsequent development. However, little is known about the regulation mechanisms of symbiosis and symbiotic germination of P. barbigerum seeds.MethodsHerein, transcriptomics and proteomics were used to explore the changes in the P. barbigerum seeds after inoculation with (FQXY019 treatment group) or without (control group) Epulorhiza sp. FQXY019 at 90 days after germination.ResultsTranscriptome sequencing revealed that a total of 10,961 differentially expressed genes (DEGs; 2,599 upregulated and 8,402 downregulated) were identified in the control and FQXY019 treatment groups. These DEGs were mainly involved in carbohydrate, fatty acid, and amino acid metabolism. Furthermore, the expression levels of candidate DEGs related to nodulin, Ca2+ signaling, and plant lectins were significantly affected in P. barbigerum in the FQXY019 treatment groups. Subsequently, tandem mass tag-based quantitative proteomics was performed to recognize the differentially expressed proteins (DEPs), and a total of 537 DEPs (220 upregulated and 317 downregulated) were identified that were enriched in processes including photosynthesis, photosynthesis-antenna proteins, and fatty acid biosynthesis and metabolism.DiscussionThis study provides novel insight on the mechanisms underlying the in vitro seed germination and protocorm development of P. barbigerum by using a compatible fungal symbiont and will benefit the reintroduction and mycorrhizal symbiotic germination of endangered orchids. |
| first_indexed | 2024-04-24T22:56:29Z |
| format | Article |
| id | doaj.art-e3618c2ff08043f3a62081b0aa6db6fd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T22:56:29Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-e3618c2ff08043f3a62081b0aa6db6fd2024-03-18T04:54:49ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13581371358137Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019Fan Tian0Fan Tian1Juncai Wang2Fangjun Ding3Fangjun Ding4Lianhui Wang5Lianhui Wang6Yanbing Yang7Yanbing Yang8Xinxiang Bai9Chengjiang Tan10Xiaofeng Liao11Guizhou Academy of Forestry, Guiyang, Guizhou, ChinaKey Laboratory for Biodiversity Conservation in the Karst Mountain Area of Southwestern China, National Forestry and Grassland Administration, Guiyang, Guizhou, ChinaGuizhou Academy of Sciences, Guiyang, Guizhou, ChinaGuizhou Academy of Forestry, Guiyang, Guizhou, ChinaKey Laboratory for Biodiversity Conservation in the Karst Mountain Area of Southwestern China, National Forestry and Grassland Administration, Guiyang, Guizhou, ChinaGuizhou Academy of Forestry, Guiyang, Guizhou, ChinaKey Laboratory for Biodiversity Conservation in the Karst Mountain Area of Southwestern China, National Forestry and Grassland Administration, Guiyang, Guizhou, ChinaGuizhou Academy of Forestry, Guiyang, Guizhou, ChinaKey Laboratory for Biodiversity Conservation in the Karst Mountain Area of Southwestern China, National Forestry and Grassland Administration, Guiyang, Guizhou, ChinaCollege of Forestry, Guizhou University, Guiyang, Guizhou, ChinaGuizhou Maolan National Nature Reserve Administration, Libo, Guizhou, ChinaGuizhou Academy of Sciences, Guiyang, Guizhou, ChinaIntroductionPaphiopedilum barbigerum is currently the rarest and most endangered species of orchids in China and has significant ornamental value. The mature seeds of P. barbigerum are difficult to germinate owing to the absence of an endosperm and are highly dependent on mycorrhizal fungi for germination and subsequent development. However, little is known about the regulation mechanisms of symbiosis and symbiotic germination of P. barbigerum seeds.MethodsHerein, transcriptomics and proteomics were used to explore the changes in the P. barbigerum seeds after inoculation with (FQXY019 treatment group) or without (control group) Epulorhiza sp. FQXY019 at 90 days after germination.ResultsTranscriptome sequencing revealed that a total of 10,961 differentially expressed genes (DEGs; 2,599 upregulated and 8,402 downregulated) were identified in the control and FQXY019 treatment groups. These DEGs were mainly involved in carbohydrate, fatty acid, and amino acid metabolism. Furthermore, the expression levels of candidate DEGs related to nodulin, Ca2+ signaling, and plant lectins were significantly affected in P. barbigerum in the FQXY019 treatment groups. Subsequently, tandem mass tag-based quantitative proteomics was performed to recognize the differentially expressed proteins (DEPs), and a total of 537 DEPs (220 upregulated and 317 downregulated) were identified that were enriched in processes including photosynthesis, photosynthesis-antenna proteins, and fatty acid biosynthesis and metabolism.DiscussionThis study provides novel insight on the mechanisms underlying the in vitro seed germination and protocorm development of P. barbigerum by using a compatible fungal symbiont and will benefit the reintroduction and mycorrhizal symbiotic germination of endangered orchids.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358137/fullPaphiopedilum barbigerummycorrhizal fungisymbiotic germinationtranscriptomeproteome |
| spellingShingle | Fan Tian Fan Tian Juncai Wang Fangjun Ding Fangjun Ding Lianhui Wang Lianhui Wang Yanbing Yang Yanbing Yang Xinxiang Bai Chengjiang Tan Xiaofeng Liao Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 Frontiers in Microbiology Paphiopedilum barbigerum mycorrhizal fungi symbiotic germination transcriptome proteome |
| title | Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 |
| title_full | Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 |
| title_fullStr | Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 |
| title_full_unstemmed | Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 |
| title_short | Comparative transcriptomics and proteomics analysis of the symbiotic germination of Paphiopedilum barbigerum with Epulorhiza sp. FQXY019 |
| title_sort | comparative transcriptomics and proteomics analysis of the symbiotic germination of paphiopedilum barbigerum with epulorhiza sp fqxy019 |
| topic | Paphiopedilum barbigerum mycorrhizal fungi symbiotic germination transcriptome proteome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1358137/full |
| work_keys_str_mv | AT fantian comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT fantian comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT juncaiwang comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT fangjunding comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT fangjunding comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT lianhuiwang comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT lianhuiwang comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT yanbingyang comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT yanbingyang comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT xinxiangbai comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT chengjiangtan comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 AT xiaofengliao comparativetranscriptomicsandproteomicsanalysisofthesymbioticgerminationofpaphiopedilumbarbigerumwithepulorhizaspfqxy019 |