6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells
6-(Methylsulfinyl) hexyl isothiocyanate (6-MSITC) is a bioactive substance found in wasabi (Wasabia japonica) and has been reported to have some bioactive effects including anticancer and antioxidant effects. However, there are no reports on its effects on periodontal resident cells, and many points...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-06-01
|
Series: | Current Issues in Molecular Biology |
Subjects: | |
Online Access: | https://www.mdpi.com/1467-3045/44/7/201 |
_version_ | 1797411993411387392 |
---|---|
author | Masahiro Shimoyama Yoshitaka Hosokawa Ikuko Hosokawa Kazumi Ozaki Keiichi Hosaka |
author_facet | Masahiro Shimoyama Yoshitaka Hosokawa Ikuko Hosokawa Kazumi Ozaki Keiichi Hosaka |
author_sort | Masahiro Shimoyama |
collection | DOAJ |
description | 6-(Methylsulfinyl) hexyl isothiocyanate (6-MSITC) is a bioactive substance found in wasabi (Wasabia japonica) and has been reported to have some bioactive effects including anticancer and antioxidant effects. However, there are no reports on its effects on periodontal resident cells, and many points remain unclear. In this study, we aimed to investigate whether 6-MSITC exerts anti-inflammatory effects on human oral epithelial cells, including effects on signal transduction pathway activation. 6-MSITC inhibited interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10) production in TNF-α-stimulated TR146 cells, which are a human oral epithelial cell line. Moreover, we found that 6-MSITC could suppress signal transducer and activator of transcription (STAT)3, nuclear factor (NF)-κB, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways activation in TNF-α-stimulated TR146 cells. Furthermore, STAT3 and NF-κB inhibitors could suppress IL-6 and CXCL10 production in TNF-α-treated TR146 cells. In summary, 6-MSITC could decrease IL-6 and CXCL10 production in human oral epithelial cell by inhibiting STAT3 and NF-κB activation. |
first_indexed | 2024-03-09T04:54:35Z |
format | Article |
id | doaj.art-e3640d084b8840a3ba4e5537e84c513d |
institution | Directory Open Access Journal |
issn | 1467-3037 1467-3045 |
language | English |
last_indexed | 2024-03-09T04:54:35Z |
publishDate | 2022-06-01 |
publisher | MDPI AG |
record_format | Article |
series | Current Issues in Molecular Biology |
spelling | doaj.art-e3640d084b8840a3ba4e5537e84c513d2023-12-03T13:07:56ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-06-014472915292210.3390/cimb440702016-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial CellsMasahiro Shimoyama0Yoshitaka Hosokawa1Ikuko Hosokawa2Kazumi Ozaki3Keiichi Hosaka4Department of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, JapanDepartment of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, JapanDepartment of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, JapanDepartment of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, JapanDepartment of Regenerative Dental Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8504, Japan6-(Methylsulfinyl) hexyl isothiocyanate (6-MSITC) is a bioactive substance found in wasabi (Wasabia japonica) and has been reported to have some bioactive effects including anticancer and antioxidant effects. However, there are no reports on its effects on periodontal resident cells, and many points remain unclear. In this study, we aimed to investigate whether 6-MSITC exerts anti-inflammatory effects on human oral epithelial cells, including effects on signal transduction pathway activation. 6-MSITC inhibited interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10) production in TNF-α-stimulated TR146 cells, which are a human oral epithelial cell line. Moreover, we found that 6-MSITC could suppress signal transducer and activator of transcription (STAT)3, nuclear factor (NF)-κB, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways activation in TNF-α-stimulated TR146 cells. Furthermore, STAT3 and NF-κB inhibitors could suppress IL-6 and CXCL10 production in TNF-α-treated TR146 cells. In summary, 6-MSITC could decrease IL-6 and CXCL10 production in human oral epithelial cell by inhibiting STAT3 and NF-κB activation.https://www.mdpi.com/1467-3045/44/7/2016-MSITCanti-inflammatory effectoral epithelial cells |
spellingShingle | Masahiro Shimoyama Yoshitaka Hosokawa Ikuko Hosokawa Kazumi Ozaki Keiichi Hosaka 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells Current Issues in Molecular Biology 6-MSITC anti-inflammatory effect oral epithelial cells |
title | 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells |
title_full | 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells |
title_fullStr | 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells |
title_full_unstemmed | 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells |
title_short | 6-(Methylsulfinyl) Hexyl Isothiocyanate Inhibits IL-6 and CXCL10 Production in TNF-α-Stimulated Human Oral Epithelial Cells |
title_sort | 6 methylsulfinyl hexyl isothiocyanate inhibits il 6 and cxcl10 production in tnf α stimulated human oral epithelial cells |
topic | 6-MSITC anti-inflammatory effect oral epithelial cells |
url | https://www.mdpi.com/1467-3045/44/7/201 |
work_keys_str_mv | AT masahiroshimoyama 6methylsulfinylhexylisothiocyanateinhibitsil6andcxcl10productionintnfastimulatedhumanoralepithelialcells AT yoshitakahosokawa 6methylsulfinylhexylisothiocyanateinhibitsil6andcxcl10productionintnfastimulatedhumanoralepithelialcells AT ikukohosokawa 6methylsulfinylhexylisothiocyanateinhibitsil6andcxcl10productionintnfastimulatedhumanoralepithelialcells AT kazumiozaki 6methylsulfinylhexylisothiocyanateinhibitsil6andcxcl10productionintnfastimulatedhumanoralepithelialcells AT keiichihosaka 6methylsulfinylhexylisothiocyanateinhibitsil6andcxcl10productionintnfastimulatedhumanoralepithelialcells |