Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells
Abstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2016-01-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100224&lng=en&tlng=en |
| _version_ | 1819180241239670784 |
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| author | Lucas da Fonseca Roberti GARCIA Alailson Domingos dos SANTOS João Carlos Silos MORAES Carlos Alberto de Souza COSTA |
| author_facet | Lucas da Fonseca Roberti GARCIA Alailson Domingos dos SANTOS João Carlos Silos MORAES Carlos Alberto de Souza COSTA |
| author_sort | Lucas da Fonseca Roberti GARCIA |
| collection | DOAJ |
| description | Abstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with the following cements: white MTA, white and gray CER and experimental white MTA. The samples were immersed in serum-free culture medium for 24 hours or 7 days (n = 16). The extracts (culture medium + components released from the cements) were applied for 24 hours to previously cultured cells (40.000 cells/cm2) in the wells of 24-well plates. Cells seeded in complete culture medium were used as a negative control. Cell viability was assessed using the MTT assay. Two samples of each cement were used for cell morphology analysis by Scanning Electron Microscopy (SEM). The extracts obtained at the 7-day period presented higher cytotoxicity compared with the 24-hour period (p < 0.05). The gray CER obtained at 24 hours presented the highest cytotoxic effect, whereas the experimental white MTA presented the lowest, similar to the control (p > 0.05). However, at the 7-day period, the experimental white MTA presented no significant difference in comparison with the other cements (p > 0.05). At the 7-day period, CER cement presented cytotoxic effects on fibroblast-like cells, similar to different MTA formulations. However, the immersion period in the culture medium influenced the cytotoxicity of the cements, which was greater for CER cement at 24 hours. |
| first_indexed | 2024-12-22T22:11:12Z |
| format | Article |
| id | doaj.art-e365e134fcc540cc9ae9c7aa1b723aea |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-22T22:11:12Z |
| publishDate | 2016-01-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-e365e134fcc540cc9ae9c7aa1b723aea2022-12-21T18:10:53ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072016-01-0130110.1590/1807-3107BOR-2016.vol30.0028S1806-83242016000100224Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cellsLucas da Fonseca Roberti GARCIAAlailson Domingos dos SANTOSJoão Carlos Silos MORAESCarlos Alberto de Souza COSTAAbstract The present study aimed at evaluating the cytotoxic effects of a novel cement called CER on periodontal fibroblast-like cells of mice (MDPL-20), in comparison with different formulations of Mineral Trioxide Aggregate (MTA), by means of the cell viability test (MTT) and cell morphology analysis. Thirty-two round-shaped samples were fabricated with the following cements: white MTA, white and gray CER and experimental white MTA. The samples were immersed in serum-free culture medium for 24 hours or 7 days (n = 16). The extracts (culture medium + components released from the cements) were applied for 24 hours to previously cultured cells (40.000 cells/cm2) in the wells of 24-well plates. Cells seeded in complete culture medium were used as a negative control. Cell viability was assessed using the MTT assay. Two samples of each cement were used for cell morphology analysis by Scanning Electron Microscopy (SEM). The extracts obtained at the 7-day period presented higher cytotoxicity compared with the 24-hour period (p < 0.05). The gray CER obtained at 24 hours presented the highest cytotoxic effect, whereas the experimental white MTA presented the lowest, similar to the control (p > 0.05). However, at the 7-day period, the experimental white MTA presented no significant difference in comparison with the other cements (p > 0.05). At the 7-day period, CER cement presented cytotoxic effects on fibroblast-like cells, similar to different MTA formulations. However, the immersion period in the culture medium influenced the cytotoxicity of the cements, which was greater for CER cement at 24 hours.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100224&lng=en&tlng=enBiocompatible MaterialsDental MaterialsEndodonticsSilicate Cement |
| spellingShingle | Lucas da Fonseca Roberti GARCIA Alailson Domingos dos SANTOS João Carlos Silos MORAES Carlos Alberto de Souza COSTA Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells Brazilian Oral Research Biocompatible Materials Dental Materials Endodontics Silicate Cement |
| title | Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells |
| title_full | Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells |
| title_fullStr | Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells |
| title_full_unstemmed | Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells |
| title_short | Cytotoxic effects of new MTA-based cement formulations on fibroblast-like MDPL-20 cells |
| title_sort | cytotoxic effects of new mta based cement formulations on fibroblast like mdpl 20 cells |
| topic | Biocompatible Materials Dental Materials Endodontics Silicate Cement |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100224&lng=en&tlng=en |
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