Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia
Abstract Background Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the terri...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1542-3 |
| _version_ | 1818205476898209792 |
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| author | Sergey V. Pisarenko Eugene I. Eremenko Alla G. Ryazanova Dmitry A. Kovalev Nina P. Buravtseva Lyudmila Yu. Aksenova Anna Yu. Evchenko Olga V. Semenova Olga V. Bobrisheva Irina V. Kuznetsova Tatyana M. Golovinskaya Dmitriy K. Tchmerenko Alexander N. Kulichenko Vitaliy Yu. Morozov |
| author_facet | Sergey V. Pisarenko Eugene I. Eremenko Alla G. Ryazanova Dmitry A. Kovalev Nina P. Buravtseva Lyudmila Yu. Aksenova Anna Yu. Evchenko Olga V. Semenova Olga V. Bobrisheva Irina V. Kuznetsova Tatyana M. Golovinskaya Dmitriy K. Tchmerenko Alexander N. Kulichenko Vitaliy Yu. Morozov |
| author_sort | Sergey V. Pisarenko |
| collection | DOAJ |
| description | Abstract Background Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region. Results We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world. Conclusions In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia. |
| first_indexed | 2024-12-12T03:57:45Z |
| format | Article |
| id | doaj.art-e3671e4f56fb41538d104406049eda1e |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-12T03:57:45Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-e3671e4f56fb41538d104406049eda1e2022-12-22T00:39:12ZengBMCBMC Microbiology1471-21802019-07-011911910.1186/s12866-019-1542-3Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in RussiaSergey V. Pisarenko0Eugene I. Eremenko1Alla G. Ryazanova2Dmitry A. Kovalev3Nina P. Buravtseva4Lyudmila Yu. Aksenova5Anna Yu. Evchenko6Olga V. Semenova7Olga V. Bobrisheva8Irina V. Kuznetsova9Tatyana M. Golovinskaya10Dmitriy K. Tchmerenko11Alexander N. Kulichenko12Vitaliy Yu. Morozov13Stavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol Research Anti-Plague InstituteStavropol State Agrarian UniversityAbstract Background Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region. Results We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world. Conclusions In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia.http://link.springer.com/article/10.1186/s12866-019-1542-3Bacillus anthracisRussiaWhole genome sequencing (WGS)Whole-genome single-nucleotide-polymorphism analysis (wgSNP)Comparative genomicsSingle nucleotide polymorphism (SNP) |
| spellingShingle | Sergey V. Pisarenko Eugene I. Eremenko Alla G. Ryazanova Dmitry A. Kovalev Nina P. Buravtseva Lyudmila Yu. Aksenova Anna Yu. Evchenko Olga V. Semenova Olga V. Bobrisheva Irina V. Kuznetsova Tatyana M. Golovinskaya Dmitriy K. Tchmerenko Alexander N. Kulichenko Vitaliy Yu. Morozov Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia BMC Microbiology Bacillus anthracis Russia Whole genome sequencing (WGS) Whole-genome single-nucleotide-polymorphism analysis (wgSNP) Comparative genomics Single nucleotide polymorphism (SNP) |
| title | Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia |
| title_full | Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia |
| title_fullStr | Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia |
| title_full_unstemmed | Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia |
| title_short | Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia |
| title_sort | genotyping and phylogenetic location of one clinical isolate of bacillus anthracis isolated from a human in russia |
| topic | Bacillus anthracis Russia Whole genome sequencing (WGS) Whole-genome single-nucleotide-polymorphism analysis (wgSNP) Comparative genomics Single nucleotide polymorphism (SNP) |
| url | http://link.springer.com/article/10.1186/s12866-019-1542-3 |
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